• 상하수도학회지
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• 제12권1호
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• pp.52-61
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• 1998

Nitrification and denitrification are important processes in the landfill site as they are deeply related with degradation and stabilization of refuse. Also nitrous oxide ($N_2O$) which is released from both nitrification and denitrification is known as greenhouse gas (GHG). The purpose of this study was to clarify the process by which $N_2O$ produced using $^{15}N$ isotope. Nitrate which was labeled to 10.08% with $^{15}KNO_3$ was used and $N_2O$ was analyzed with GC mass. Results was that even also when $O_2$ of bulk was 15%, $N_2O$ was released from denitrification. And as concentrations of $O_2$ increase, sum of $N_2O$ was released from denitrification. And as concentrations of $O_2$ increase, sum of $N_2O$ and $N_2$ was decreased and ratios of $N_2O$ in the reduced gases were increased. FISH technics also adaped to confirm whether which of nitrifiers existed in the substrates. When NEU was used of which the target was ammonia oxidizing bacteria, nitrifier was not detected at all. So it was confirmed that during the reaction denitrification was dominant process. Total bacteria distributions which were detected by EUB probe explained that as $O_2$ increase the number of bacteria also increase, but between the 10-15% of $O_2$ there was no any differences.

• JSTS:Journal of Semiconductor Technology and Science
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• 제15권1호
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• pp.41-47
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• 2015

In this paper, the effective electron Schottky barrier height (${\Phi}_{Bn}$) of the Ni silicide/n-silicon (100) interface was studied in accordance with different thicknesses of the antimony (Sb) interlayer for high performance n-channel MOSFETs. The Sb interlayers, varying its thickness from 2 nm to 10 nm, were deposited by radio frequency (RF) sputtering on lightly doped n-type Si (100), followed by the in situ deposition of Ni/TiN (15/10 nm). It is found that the sample with a thicker Sb interlayer shows stronger ohmic characteristics than the control sample without the Sb interlayer. These results show that the effective ${\Phi}_{Bn}$ is considerably lowered by the influence of the Sb interlayer. However, the current level difference between Schottky diodes fabricated with Sb/Ni/TiN (8/15/10 nm) and Sb/Ni/TiN (10/15/10 nm) structures is almost same. Therefore, considering the process time and cost, it can be said that the optimal thickness of the Sb interlayer is 8 nm. The effective ${\Phi}_{Bn}$ of 0.076 eV was achieved for the Schottky diode with Sb/Ni/TiN (8/15/10 nm) structure. Therefore, this technology is suitable for high performance n-channel MOSFETs.

• 한국작물학회지
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• 제46권4호
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• pp.303-308
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• 2001

To investigate the partitioning of newly absorbed N derived from NO$_3$- and NH$_4$$^{+}$, 6 mM $K^{15}$ NO$_3$ or 3 mM ($^{15}$ NH$_4$)$_2$ was fed continuously in Italian ryegrass (Lolium multiflrum L.) for 7 days. Nitrogen metabolites (nitrate, amino acid, soluble- and insoluble protein) were analyzed at the end of $^{15}$ N feeding. Dry weight in shoot, stubble and root was not significantly different between NO$_3$$^{[-10]}$ and NH$_4$$^{+}$ feeding. Total nitrogen content in all three organs was significantly higher in NH$_4$$^{+}$ than NO$_3$$^{[-10]}$ feeding. Sum on N content in reduced N fractions (amino acids + proteins) in shoot, stubble and roots in NH$_4$$^{+}$ feeding increased by 13.3, 12.5 and 35.4 %, respectively, compared to NO$_3$$^{[-10]}$ feeding. The Relative Specific Activity (RSA, percentage of newly absorbed $^{15}$ N relative to total N in a sample) values of amino acids and insoluble proteins were significantly higher in NH$_4$$^{+}$ feeding. Total amount of newly absorbed $^{15}$ N in NO$_3$$^{[-10]}$ and NO$_3$$^{[-10]}$ feeding was 52.3 and 69.5 mg/plant on dry matter basis, respectively. In both NH$_4$$^{+}$ and NO$_3$$^{[-10]}$ grown plants, most of the N was allocated to the shoot, 67.5% in NH$_4$$^{+}$ feeding and 58.8% NO$_3$$^{[-10]}$ feeding, respectively. The $^{15}$ N amount incorporated in the reduced N compounds (amino acids and proteins) in NH$_4$$^{+}$ grown plants significantly increased by 74.8% compared to NO$_3$$^{[-10]}$ grown plants. The increase of the $^{15}$ N amount assimilated to amino acids in NH$_4$$^{+}$ grown plants was remarkably higher in roots as more than 7.25 times compared to NO$_3$$^{[-10]}$ feeding. These results indicated that Italian ryegrass was much efficiently utilized NH$_4$$^{+}$-N for the synthesis of reduced N compounds.reduced N compounds.

• Bulletin of the Korean Chemical Society
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• 제22권11호
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• pp.1197-1201
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• 2001

In the spectrum of uniformly 15N labeled cytochrome c3, the relative linewidths of the doublet peaks of the 15N-coupled imido proton of the coordinated imidazole group were reversed on oxidation. This inversion was explained by the interference relaxation process between the electron-proton dipolar and 15N-1H dipolear interactions. The inversion can be used to assign the imido protons of the coordinated imidazole groups in heme proteins.

• 한국작물학회지
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• 제50권1호
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• pp.16-21
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• 2005

This study was conducted to establish the elaborate nitrogen fertilization method to enhance N use efficiency in direct-seeded rice on flooded paddy. The nitrogen uptake by rice plants was insignificant until 25 days after seeding, and increased gradually thereafter. During this early growth stage, rice plants absorbed only the $4\%$ of basal applied N, while the $45\%$ of N fertilizer remained in the paddy soil. The absorption of basal N by rice plants was almost completed at 46 days after application. Nitrogen top-dressed at 5-leaf stage was well matched to crop nutrient demand, so it could be absorbed so actively in 8days after application. As a result, we could cut down the amount of N fertilizer to $36\%$ of the basal N level without significant difference in yield. Plant recoveries of fertilizer $^{15}N$ applied with different application timings were $7.8\%$ for basal, $9.4\%$ for 5-leaf stage, $17.1\%$ for tillering stage, and $23.4\%$ for panicle initiation stage, respectively. When urea was applied with nitrogen fertilization practice based on basal incorporation (BN), plant recovery of $^{15}N$ at harvest was $31.0\%$, which was originated from $13.7\%$ for grain, and $21.3\%$ of the fertilizer $^{15}N$ remained in the soil, and the rest could be uncounted. Plant recovery of fertilizer $^{15}N$ applied with nitrogen fertilization practice based on topdressing at 5-leaf stage (TN), where N rate was reduced by $18\%$ compared with BN, was $35.1\%$ (grain $15.6\%$), and $19.9\%$ of the fertilizer $^{15}N$ remained in the soil, and the rest could be uncounted. TN showed a higher $^{15}N$ recovery than BN because it was to apply N fertilizer at a time to well meet the demand of rice plant direct-seeded on flooded paddy. We concluded that TN would be the nitrogen fertilization method to enhance N use efficiency in direct-seeded rice on flooded paddy.

• 약학회지
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• 제28권4호
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• pp.197-206
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• 1984

The derivatives of N-(alkylcarbamoyl) amino acid methyl ester, N-(2-chloroethylcarbamoyl)-glycine methyl ester (7a), -valine methyl ester (8a), -phenylalanine methyl ester (9a), N-(N'-methylcarbamoyl)-glycine methyl ester (7b), -valine methyl ester (8b), and-phenylalanine methyl ester (9b), were prepared by reacting the corresponding free amino acid methyl ester (glycine-, valine-, phenylalanine-methyl ester) with isocyanate (R-N=C=O${\cdot};R=Cl-CH_2-CH_2-or\; CH_3-)$. The prepared N-(alkylcarbamoyl) amino acid methyl esters (7,8,9) were treated with $NaNO_2$/98% HCOOH in order to obtain their nitrosoated products, N-(alkyl-N'-nitrosocarbmoyl)amino acid methyl ester. The compound (7,8,9) gave N-(2-chloroethyl-N'-nitrosocarbamoyl)-valine methyl ester (14a),-phenylalanine methyl ester (15a), N-(N'-alkyl-N'-nitrosocarbamoyl)-glycine methyl ester (13b),-valine methyl ester. (14b), and-phenylalanine methyl ester (15b) respectively under the nitrosoation. On the other hand, N-(2-chloroethylcarbamoyl) glycine methyl ester produced N-(2-chloroethylcarbamoyl)-N-nitrosoglycine methyl ester (13a). The inhibitory activity of the prepared N-(alkylcarbamoyl) amino acid methyl ester (7,8,9) and N-(alkyl-N'-nitrosocarbamoyl) amino acid methyl ester (13,14,15) towards the growth of L1210 murine leukemia cells were examined. Among them the compound (14a) and (15a) exhibit excellent activity having $ED_{50}\; to\;be\;1.5{\mu}g/ml\;and\;3.0{\mu}g/ml respectively.

• 한국작물학회지
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• 제30권2호
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• pp.140-145
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• 1985

퇴비시용량 (0, 1,500kg/10a) 및 질소시비량 (0, 5, 10, 15, 20kg/10a)과 질소반양추비(1982년, 15, 20kg/10a의 경우)에 따른 단옥수수 Sun glow(1982년 공시) 및 Honey Bantam-9 (1983년 공시)의 생육 및 수량성은 다음과 같다. 1. 퇴비시용은 이삭의 크기를 증대시켰고 10a당이삭무게를 약 10％ 증가 시켰다. 2. 10a당 이삭수는 10a당 질소 10kg까지 증비할 때는 크게 증가하였고 이삭무게는 무퇴비구에서는 10a당 질소 20kg까지도 증수되었으나 퇴비시용구에서는 10a당 질소 10kg 까지만 큰 증수를 보였다. 3. 10a당 지상부 건물수량은 무퇴비구에서는 10a당 질소 15kg, 퇴비시용구에서는 질소 10kg까지 크게 증가 되었다. 4 지상부 질소흡수량은 무질소에서 8.1kg였고 10a당 질소 20kg 시용에서 18.8kg로 질소시비량 증가에 따라 직선적인 증가 경향이었다. 5 10a당 이삭수, 이삭무게, 건물중 등을 고려한 적정 질소시비량은 10a당 10-15kg로 보여진다. 6, 질소추비의 효과는 이삭크기, 10a당 이삭수 및 이삭무게 등에서 인정되지 않았다.

• 한국토양비료학회지
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• 제45권6호
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• pp.1094-1099
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• 2012

담수조건으로 토양질소동태에 대한 결실기 자운영의 투입효과를 포트시험을 통해 평가하였다. 자운영의 투입에 따라 벼의 자운영유래 질소 이용율은 약 51-64%으로 자운영의 투입량이 높을수록 감소하였다. 수확기 토양의 자운영질소는 약 3-5%가 저장되었고, 자운영 유래 질소의 손실은 각각 32.4와 44.1%를 차지하였다. 벼 수량 대비 자운영 $30Mg\;ha^{-1}$ 시용은 질소추천시비량을 전량 대체할 수 있었다.

• 한국초지조사료학회지
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• 제4권2호
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• pp.158-163
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• 1983

種實用 수수와 수단그라스의 交雜種인 Pioneer 988의, 窒素施肥水準에 따른 時期別 生育 및 乾物生産量을 조사하기 위하여 本 硏究를 실시하였다. 窒素肥科는 成分量으로 10a當 15, 25, 35kg을 施用하였으며 幼植物로부터 開花後期까지의 生育期間게 몇가지 特性을 調麥한바 그 結果는 다음과 같이 要約된다. 1. 出現葉數는 生育이 進行됨에 따라 증가했는데 穗孕期까지 급격한 증가를 보였고, 出穗期에는 最終葉인 15葉이 出現했다. 2. 葉面積指數는 生育의 進行에 따라 증가했는데, 특히 穗孕期까지 그 增加가 급격했으며, 室素施肥水準別로는 幼穗分化期까지 15kg 施肥區에서 컷으나 穗孕期 以後에는 35kg 施肥區에서 컷다. 3. 乾物收量은 穗孕期 以前에는 15kg 施肥區에서 많았으나, 穗孕期 以後에는 35kg 施肥區에서 많았다. 4 乾物生産速度는 純同化率 및 平均 葉面積指數와 밀접한 관계가 있었으며 生育後期에는 純同化率의 增加와 더욱 밀접한 관계를 나타냈다.

• 생태와환경
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• 제50권4호
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• pp.452-458
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• 2017

본 연구는 암모니아성 질소 및 질산성 질소의 안정동위원소 분석방법을 적용하여, 호소 내 식물플랑크톤 성장에 영향을 미치는 외부 오염원의 기원을 추정함으로써, 효율적인 수질 관리 및 수생태계 기능해석 지원기능을 제공하기 위하여 연구하였다. 남한강, 북한강, 팔당호 지역에 비해서 경안천 지역의 유기물 기원이 뚜렷하게 차이를 보이며, 외부기원 유기물이 높은 영향을 미치는 것으로 여겨진다. 또한 식물플랑크톤(규조류, 남조류)이 자생기원 보다는 외부기원 질소원을 활용하고 있음을 확인하였다. 한강 유역에서 암모니아성 질소 및 질산성 질소의 안정동위원소비를 이용한 유기물 기원 연구는 적용가능 할 것으로 여겨지며, 식물플랑크톤의 탄소 및 질소 동위원소비를 활용하여 그 거동을 추정할 수 있었다. 추후 유역 오염원의 대표 값(end member)의 지속적인 조사를 통하여 자료구축이 이루어져야 할 것이다.