• 한국식품위생안전성학회지
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• 제13권3호
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• pp.221-231
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• 1998

Chlorella ellipsoidea의 엽록체막 및 틸라코이드 막의 당지질 생합성과 그의 지방산 조성에 식품 보전제가 미치는 영향을 분석하고자 potassium sorbate(0.3 mM, P.S) , sodium bensoate(0.4mM, S.b), calcium propionate(2 mM, C.P)를 처리한 배지에서 세포를 배양한 후 배양초와 배양 중간기에 세포를 수확하여 막을 분리하고, 이들 막을 구성하고 있는 당지질 중 MGDG, DGDG의 함량 및 그의 지방산 조성을 대조구와 비교 분석하였다. Total lipid와 MGDG, DGDG 함량은 대조구와 비교해 볼 때 처리구에서 현저하게 감소하였다. 염록체막의 BGDG를 구성하는 지방산은 대조구에서는 palmitoleic acid 15.55% , oleic acid 15.09%가 이용되었으며, P.S 처리구는 linolenic acid 14.36%, oleic acid 14.71%, S.B 처리구는 palmitoleic acid 18.26%, oleic acid 17.26%, C.P 처리구는 palmitoleic acid 16.31%, oleic acid 16.88%를 당지할 합성에 주로 이용하였다. DGDG 형성에 이용된 지방산은 대조구의 경우 linolenic acid 17.74%, oleic acid 15.75%, P.S처리구는 palmitoleic acid 15.97%, oleic acid 14.90%, S.B 처리구는 oleic acid 15.74%, palmitoleic acid 13.29% C.P 처리구에서는 oleic acid 14.52%, palmitoleic acid 14.03%가 당지질 합성에 도입되었다. 틸라코이ㅡ막에서의 당지질 중 MGDG 생합성에 이용되는 지방산은 대조구에서는 linolenic acid 가 14.76%, oleic acid가 12.90% 사용되었으며 P.S 처리구에서는 palmitic acid와 palmitoleic acid가 각각 13.00%씩 이용되었다. S.B 처리구는 palmitoleic acid 12.94%, oleic acid 12.44% , C.P 처리구는 oleic acid 13.27 %, palmitoleic acid 9.66%를 당지질 생합성에 이용하였다. DGDG 합성에 이용되는 지방산은 대조구의 경우 linolenic acid 18.01% , oleic acid 15.53%로 나타났으며, P.S 처리구는 linolenic acid 19.20%, linoleic acid 14.14%, S.B 처리구는 oleic acid 14.85%, palmitoleic acid 9.03%, C.P 처리구는 linolenic acid 12.66%, olei acid 13.90%를 주로 이용하였다.

• 분석과학
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• 제21권3호
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• pp.212-221
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• 2008

사건현장에 잔류하는 잠재지문의 효과적인 탐색 및 감식을 위한 자료를 얻고자 비다공성 증거물에 잔류된 잠재지문의 지방산 조성분석을 실시하고 7개월간의 지문유류에 따른 조성 변화에 대하여 알아보고자 하였다. 지문시료는 한국인 29-50 세 남성 8인과 여성(36 세) 1명으로부터 제공받았다. 모든 지문에서는 lauric acid (C12:0), myristic acid (C14:0), palmitic acid (C16:0), stearic acid (C18:0), elaidic acid (C18:1n9t), oleic acid (C18:1n9c), linoleic acid (C18:2n6c), arachidic acid (C20:0), linolenic acid (C18:3n3), erucic acid (C22:1n9)와 docosadienoic acid (C22:2)의 11종의 지방산이 검출됨을 확인하였고, palmitic acid (35.45-48.37%), oleic acid (14.84-28.49%), stearic acid (9.71-24.96%)와 linoleic acid (7.68-18.85%)가 확인된 지방산조성의 75%이상을 차지하여 주요 구성성분임을 알 수 있었다. 또한 7개월간 온도 $20-25^{\circ}C$, 습도 40-50%의암실환경에서의장기간유류에따라총지방산의함량이 12-25% 감소하였으며, 이는 elaidic acid (C18:1n9t), arachidic acid (C20:0), linolenic acid (C18:3n3), erucic acid (C22:1n9), docosadienoic acid (C22:2)의 장쇄지방산들의 소실과 단쇄지방산인 myristic acid (C14:0), myristoleic acid (C14:1), pentadecanoic acid (C15:0)의 생성에 따른 결과임을 확인하였다.

• Asian-Australasian Journal of Animal Sciences
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• 제11권1호
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• pp.60-64
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• 1998

The different developmental stage embryos and oviductal and uterine fluids of rabbit were analyzed by gas chromatography. Myristic (C 14:0), palmitic (C 16:0), palmitoleic (C 16:1), stearic (C 18:0), oleic (C 18:1), linoleic (C 18:2), linolenic (C 18:3), arachidic (C 20:0), arachidonic (C 20:4), docosahexaenoic (C 22:6) and lignoceric (C 24:0) acids were the common fatty acid constituents with little exception. In most of the samples palmitic, oleic, linoleic and arachidonic acids were observed in high concentration. Moreover, linoleic, linolenic and arachidonic acids were the three poly-unsaturated fatty acids in both type sample except day-1 oviductal fluids. Similarly, in both day-1 and day-2 oviductal and uterine fluids myristic, palmitoleic, stearic, linolenic, arachidic and docosahexaenoic acids were in less composition or undetected.

• Asian-Australasian Journal of Animal Sciences
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• 제10권5호
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• pp.523-527
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• 1997

The fatty acid compositions of oocytes, follicular, oviductal and uterine fluids of pig and cow were analyzed using gas chromatography. Myristic (C 14: 0), palmitic (C 16: 0), palmitoleic (C 16: 1), stearic (C 18 : 0), oleic (C 18: 1), linoleic (C 18: 2), linolenic (C 18: 3) and arachidonic (C 20: 4) acids were identified as the common fatty acid constituents with little exception. Oleic acid composition was the highest (21.90 to 36.24%) in both pig and cow followed by palmitic (18.61 to 31.90%) and stearic (10.34 to 20.39%) acid. The three polyunsaturated fatty acids like linoleic, linolenic and arachidonic acids were detected in both pig and cow reproductive fluid samples. Myristic acid was not detected in pig oviductal fluid. Similarly, in cow oocytes myristic, palmitoleic and linolenic acids were not detected. Moreover, palmitic, stearic, oleic and linoleic acid comprised about 80% (73.74 to 88.00%) of the total fatty acids in the different samples analyzed in both animals.

• 한국수정란이식학회지
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• 제15권2호
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• pp.147-156
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• 2000

To investigate fatty acid constituents and relative compositions in the fluid of the follicles, oviducts, uterine body and uterine body in Korean native cow, the fluids of the reproductive tract were analyzed using gas chromatography. The samples were taken from various reproductive tract of 23 Korean native cows. q. Caprylic acid (C8:0), myristic acid(C14:0), palmitic acid(C16:0), palmitoleic acid(C16:1), stearic acid(C18:0), oleic acid(C18:1), linoleic acid(C18:2), arachidonic acid(C20:4) were found in the reproductive tracts of the cows, which made 8 kinds of fatty acid in total. 2. Palmitic acid, oleic acid, stearic acid were predominant with 35.67%, 24.98% and 17.52%, respectively. while low levels of fatty acids(<5%) were myristic acid, palmitoleic acid and caprylic acid with 1.75%, 1.28% and 2.69%, respectively. 3. Two kinds of polyunsaturated fatty acids, linoleic acid arachidonic acid were found in the reproductive tracts of cows. 4. Palmitic acid among saturated fatty acids and oleic acid among unsaturated fatty acids were the highest level in all of the reproductive tracts. 5. The Highest level of arachidonic acid was found in the uterine horn. 6. The sum of the palmitic acid and oleic acid were 61.72%, 63.72%, 57.66% and 57.65% for the fluid of follicle, oviduct, uterine horn and uterine body of the cows, respectively. 7. The relative compositions of palmitic acid, palmitoleic acid and caprylic acid were higher during the luteal phase than during the follicular phase. 8. The relative compositions of arachidonic acid was higher during the follicular phase in the fluid of uterine horn and uterine body of the cows. 9. The long chain fatty acid, the palmitic acid, stearic acid, oleic acid and linoleic acidshowed higher relative compositions during the follicular phase(86.49%∼95.51%) than during the luteal phase(85.64%∼88.93%).

• 한국식품영양과학회지
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• 제15권2호
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• pp.115-118
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• 1986

한국산 식용(食用)지렁이(Lumbricus terrestris)를 동결건조시킨 후 분말로하여 chloroform-methanol(2:1, v/v)로 추출한 총지질의 정량 및 silicic acid column chromatography로 중성지질, 당지질, 인지질로 분획하여 gas chromatography에 의하여 이들 각 획분별 지방산 조성을 분석한 결과를 요약하면 다음과 같다. 동결건조 지렁이(수분함량 4.68%)를 추출한 총지질은 10.80%였고, Folch법에 의해 정제된 양은 68.5%였다. Silicic acid column chromatography로 분획한 결과 중성지질은 35.14%, 당지질이 41.74%였으며, 인지질은 23.12%였다. 각지질 획분별, 지방산 조성을 분석한 결과는 중성지질의 포화지방산은 60.01%, monoene산이 25.38%, polyene산이 11.30%이었으며, 지방산중 lauric acid가 15.63%로 가장 많았고, oleic acid가 13.54%로 되어 있었으며, 당지질은 포화지방산이 58.41%, monoene산이 8.67%, polyene산이 5.39%였고, C수가 10미만인 지방산이 23.22%였다. caproic acid가 25.80%로 가장 많았고 다음이 lauric acid로 11.22%였었다. 또한 인지질은 포화지방산이 44.75%, monoene산이 17.88%, polyene산이 25.24%로 중성당지질보다 polyene산이 많았었다. oleic acid가 16.20%로 가장 많은 양을 나타내었고, 다음이 caproic acid로 12.07%였었다.

• 한국수정란이식학회지
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• 제15권2호
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• pp.137-145
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• 2000

To investigate fatty acid constituents and relative compositions in the fluid of the follicles, oviducts, uterine ho군 and uterine body in sows, the fluids of the reproductive tract were analyzed using Gas chromatography. The samples were taken from various reproductive tract of 21 sows slaughtered. 1. Caprylic acid(C8: 0), capric acid(C10:0), lauric acid(C12:0), myristic acid(C14:0), palmitic acid(C16:0), plamitolele acid(C16:1), stearic acid(C18:0), oleic acid(C18:1), linoleic acid(C18:2) and arachidonic acid(C20:4) were found in the reproductive tracts of the sows, which made 10 kinds of fatty acid intotal. 2. Two kinds of polyunsaturated fatty acids, linoleic acid and archidonic acid were found inthe reproductive tracts. 3. Palmitic acid among saturated fatty acids and oleic acid among unsaturated fatty acids were the hihgest level in all of the reproductive tracts. 4. Palmitic acid, oleic acid and stearic acid showed higher rate with 44.89%, 23.69% and 14.36%, respectively, and lauric acid, capric acid, palmitoleic acid, arachidonic acid ad myristic acid showed lower rate with 0.62%, 1.13%, 1.65%, 1.97% and 2.24%, respectively in the reproductive fluid. 5. The highest level of arachidonic acid was found in the uterine horn. 6. The sum of the palmitic acid and oleic acid were 66.91%, 70.41%, 66.14% and 73.36% in the fluid of follicle, oviduct, uterine horn and uterine body, respectively. 7. The relative composition of arachidonic acid was higher during the follicular stage than during the luteal phase in the fluid of oviduct and uterine. 8. The long chain fatty acids such as the palmitic acid, stearic acid, oleic acid and linoleic acid showed higher relative compositions during the follicular phase(93.18%∼96.83%) than during the luteal phase(82.56%∼88.37%). 9. Caprylic acid, luric acid and palmitoleic acid were undetected in the fluid of all of the reproductive tracts during the follicular phase. Low relative compositions of capric acid, myristic acid andarachidonic acid were found during the follicular phase, while the low relative compositions (<5%)of capric acid, lauric acid, myristic acid, plamitoleic acid and arachidonic acid were found during the luteal phase.

• The Korean Journal of Ecology
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• 제19권1호
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• pp.21-29
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• 1996

Variation in triacylglycerols(TGs) and fatty acids in Fucus serratus was analyzed for a period of one year. TGs were more concentrated during the summer(2.8mg/g dw)and autumn(2.6mg/g dw) than during the spring (0,7mg/g dw)and winter (0.5mg/g dw). The dominant fatty acides in total liqid were palmitic acid ($C_{16:0}$, 24.1%), oleic acid (($C_{18:1}$, 22.4%) and arachidonic acid (($C_{20:4}$, 14.4%) but the dominant ones in TG were $C_{16:0}$(22.8%), $C_{18:1}$(36.4%) and $C_{18:2}$(linoleic acid, 16.4%). The levels of $C_16$ fatty acids were high in winter while $C_18$ in summer and autumn. The polyunsaturated fatty acids (UFAs) were more abundant in the $C_20$ series, while the UFAs of the $C_16$ were low. Especially, the amount of arachidonic acid ($C_{20:4}$, 14.4% of total fatty acids (TFA) was more abundant than that of eicosapentaenoic acid ($C_{20:5}$, 10.4% of TFA). The amount of $C_{20:4}$ and $C_{20:5}$,in TG was 9.2% and 4.8%, respectively. These UFAs in total lipid were thus higher than TG. Therefore, the synthesis of TG and fatty acid was stimulated by the alternation of emersion and submersion of thalli from sea water and eco-physiological conditions during summer: high temperature and light, and low concentration of nitrogen.

• Journal of Nutrition and Health
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• 제15권1호
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• pp.15-21
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• 1982

In this work, the quantitative estimation of fatty acids in sesame and perilla oil by high performance liquid chromatography (HPLC) was investigated. The analysis of fatty acids were separated by HPLC using a differential refractometer as a detector. With micro Bondapak $C_{18}FFAA$ column and acetonitril, chloroform and tetrahydrofuran mixture as a solvent. In the fatty acid compositions, sesame oil was composed mainly of linoleic and oleic acids 49.6 % and 34.7%. In perilla oil, the amounts of oleic, linoleic and linolenic acids were 13.6%, 14.5% and 63.8%, respectively.

• 한국식품과학회지
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• 제21권5호
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• pp.697-705
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• 1989

생 율무가루와 가공(침지, 증자, 건조)된 율무가루를 $5^{\circ}C$ 및 $35^{\circ}C$에서 6개월 동안 저장하면서 지방질에 있는 지방산 조성의 변화를 조사하였다. 각 획분별 지방질의 주요지방산은 oleic, linoleic, palmitic acid였으며, stearic 및 linolenic acid도 소량으로 함유되어 있었다. $35^{\circ}C$에서 저장하는 동안에 linoleic acid의 조성비는 감소하였으며 상대적으로 oleic 및 palmitic acid의 조성비는 증가하였으나, $5^{\circ}C$에서는 거의 변화가 없었으며, 생 율무가루가 가공된 율무가루보다도 더욱 현저하게 변화하였다. TG에 함유된 지방산 조성의 변화는 중성지방질과 거의 유사하였으며, 유리지방산은 $35^{\circ}C$에서 저장한 것이 $5^{\circ}C$에서 저장한 것보다 oleic acid를 많이 함유한 반면, linoleic acid를 적게 함유하였으며 이러한 현장은 생 시료에서 더 현저하였다.