• 대한핵의학회:학술대회논문집
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• 1998.11a
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• pp.32.2-32.2
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• 1998

• 한국생물공학회:학술대회논문집
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• 2000.11a
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• pp.202-205
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• 2000

A gene coding $endo-{\beta}$,-1, 4 glucanase from Actinomyces sp. KNG40 and phytase from Hansenula polymorpha were cloned into Esherichia coli JM101 by using E. coli/Lactobacillus shuttle vector pNZ3004 and pNZ123. The plasmid p3PS(1-4) and p123(1-4) have slpA promoter and slpA signal sequence. So, I constructed expression vectors, p3PS(1-4)Endo, phy and p123(1-4)Endo, phy. These constructed vector was transformed in target host Lactobacillus gasseri and reutri. These transformed host expressed endoglucanase and phytase as extracellular fraction. In the enzyme activity of the same vector, host L, gasseri was higher activity than L. reuteri. This indicates that L. gasseri recongnize promoter and signal sequence very well.

• Journal of the Korea Convergence Society
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• v.9 no.7
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• pp.87-93
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• 2018

In this study, $^{99m}Tc$, $^{123}I$, $^{201}Tl$, $^{18}F$, and $^{131}I$, which are widely used in nuclear medicine, were transmitted through a bismuth shield. We investigated the shielding rates according to the type of radioisotope and the distance of measurement. For the experiment, 6 sheets of lead equivalent 0.25 mm Pb of bismuth shielding material were stacked one by one up to 1.50 mm as the thickness increased. The distance was 30 cm, 50 cm, and 100 cm, and the transmission dose was measured. As a result, the shielding rates was measured as the thickness increased, and the measured value decreased as the distance increased. The shielding rate of $^{123}I$ and $^{201}Tl$ was higher than $^{99m}Tc$, $^{18}F$ and $^{131}I$ showed lower shielding effect when there is a shielding material than when there is no shielding material due to high energy and ${\beta}$ rays. Based on the results of experiments, it would be helpful to reduce the exposure of nuclear medicine workers and to manage the exposure if bismuth shields are used depending on the type of radioisotope.

• 대한방사선방어학회:학술대회논문집
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• 2011.11a
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• pp.122-123
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• 2011

• Proceedings of the Membrane Society of Korea Conference
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• 2008.05a
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• pp.123-125
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• 2008

• Journal of the Korea Institute of Information and Communication Engineering
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• v.18 no.6
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• pp.1462-1468
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• 2014

Xe gas is moved to target from GPM. It is used to feasible nuclear reaction from proton of 30MeV cyclotron being investigated by the Xe-124 gas target system. This system is divided into four parts. The hardware system was constructed by solidworks 3-D CAD and Helium supply is to cool the Havor foil. The Cooling water has the job of cooling down the temperature when Xe gas is being investigated in the target. Temperature and pressure gauges are attached to be checked easily. GPM has the part that prepares to transport Xe gas. There are storage vessel that stores Xe gas, the cold trap that filters humidity and impurity and lastly storage vessel that temporarily stores Xe gas. HCS using the helium is to clean and cool for each part. These parts are configured with SIEMENS PLC and PcVue monitoring program for more comfortable and easy maintenance.

• The Journal of Internal Korean Medicine
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• v.30 no.1
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• pp.36-50
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• 2009

Objectives : This study was aimed to verify the cytoprotective function, antioxidative effect and inflammation genes inhibitory effects of Lycium chinense Milie. Therefore the generation of superoxide anion radical ( $O_2\;^-$), peroxynitrite ($ONOO^-$), nitric oxide (NO) and prostaglandin $E_2$ $(PGE_2)$ was investigated in the renal epithelial cells of mouse. Effects of Lycium chinense Milie on the expression of inflammation-related proteins, $IKK-{\alpha}$. $p-IKK-\alpha\beta$, $p-I{\kappa}B-\alpha$, $NF-{\kappa}B$ (p50, p65), COX-2 and iNOS, were examined by western blotting. Methods : For this study, the fluorescent probes were used, namely dihydrorhodamine 123 (DHR 123), 4.5-diaminofluorescein (DAF-2) and 2',7'-dichlorodihydrofluorescein diacetate (DCFDA). Western blotting was performed using anti-$IKK-\alpha$, anti-phospho $IKK-\alpha\beta$, anti-phospho $I{\kappa}B-\alpha$, anti-$NF-{\kappa}B$ (p50, p65), anti-COX-2 and anti-iNOS, respectively. Results : Lyciutn chinense Milie reduced $H_{2}O_{2}$-induced cell death dose-dependently. It inhibited the generation of $O_2\;^-$, $ONOO^-$, NO and $PGE_2$ in the $H_{2}O_{2}$-treated renal epithelial cells of mouse in vitro. Lycium chinense Milie inhibited the expression of $IKK-\alpha$, $p-IKK-\alpha\beta,\;p-I{\kappa}B-\alpha$, COX-2 and iNOS genes by means of decreasing activation of $NF-{\kappa}B$. Conclusions : According to above results. Lycium chinense Milie recommended to be applied in treatment for the inflammatory process and inflammation-related diseases.

• The Korea Journal of Herbology
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• v.36 no.5
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• pp.117-123
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• 2021

Objectives : The aim of this study is to investigate the effect of wogonin on the production of hydrogen peroxide in polyinosinic:polycytidylic acid (poly I:C)-stimulated TM4 mouse sertoli cells. Methods : TM4 were treated with poly I:C (50 ug/mL) and wogonin at concentrations of 5, 10, 25, and 50 µM for 30 min, 2 hr, 12 hr, 18 hr, and 24 hr. The production of intracellular hydrogen peroxide was measured by dihydrorhodamine 123 assay. Results : For 30 min, 2 hr, 12 hr, 18 hr, and 24 hr treatment, wogonin significantly inhibited intracellular hydrogen peroxide productions of TM4 at the concentration of 5, 10, 25, and 50 µM (p<0.05). In details, production of hydrogen peroxide in poly I:C-stimulated TM4 treated for 30 min with wogonin at concentrations of 5, 10, 25, and 50 µM was 95.67%, 92.69%, 92.05%, and 91.97% of the control group treated with poly I:C only, respectively; the production of hydrogen peroxide for 2 hr was 94.44%, 94.41%, 93%, and 92.98%, respectively; production of hydrogen peroxide for 12 hr was 96.78%, 95.32%, 94.33%, and 93.17%, respectively; production of hydrogen peroxide for 18 hr was 94.7%, 93.4%, 93.38%, and 93.35%, respectively; and production of hydrogen peroxide for 24 hr was 95.75%, 94.77%, 94.58%, and 92.8%, respectively. Conclusions : Wogonin might have anti-viral property related with its inhibition of intracellular hydrogen peroxide production in poly I:C-stimulated TM4 cells.

• The Journal of Internal Korean Medicine
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• v.29 no.2
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• pp.421-431
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• 2008

Objectives : This study was to investigate the inhibitory effects of Ulmus davidiana on the generation of peroxynitrite $(ONOO^{-})$, nitric oxide (NO) and superoxide anion radicals $(O_{2}^{-})$ in the endothelial cells of rat vessels. The effects of Ulmus davidiana on the expression of inflammation-related proteins, $NF-{\kappa}B$ (p50, p65), COX-2, and iNOS, were examined by western blotting. Methods : For this study, fluorescent probes, namely dihydrorhodamine 123 (DHR 123), 4,5-diaminofluorescein (DAF-2) and 2',7'-dichlorodihydrofluorescein diacetate (DCFDA) were used. Western blotting was performed via using anti-$NF-{\kappa}B$ (p50, p65), anti-COX-2, and anti-iNOS, respectively. Results : Ulmus davidiana inhibited the generation of $ONOO^{-}$, NO and $(O_{2}^{-})$ in the lipopolysaccharide (LPS)-treated endothelial cells of rat vessels in vitro. Ulmus davidiana inhibited the expression of COX-2 and iNOS genes by means of decreasing the $NF-{\kappa}B$ activation. Conclusions : These results suggest Ulmus davidiana is effective on inhibiting the generation of $ONOO^{-}$, NO and $O_{2}^{-}$, and that therefore it might have a potential role as a treatment for the inflammatory process and inflammation-related diseases.

• Bulletin of the Korean Chemical Society
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• v.10 no.2
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• pp.123-128
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• 1989

Calorimetric study in conjunction with Fourier-transform infrared (FTIR) spectroscopic study was carried out on the blends of poly(ethylene oxide) (PEO) with isotactic, atactic and syndiotactic poly(methyl methacrylate) (i-, a-, and s-PMMA). From the differential scanning calorimetric (DSC) measurements, the three types of blends show a depression of the melting temperatures. This indicates that PEO is compatible with i-, a-, and s-PMMA. But the largest melting point depressions of PEO are always found in the blends with s-PMMA. For PEO/a-PMMA and PEO/s-PMMA, the degree of crystallinity as a function of composition deviates substantially from that of the ideal blend in which no interaction between the components exists. The FTIR spectra of all three types of blends are recorded. In order to observe the microstructural changes of PEO in blends, we analyzed the spectra using digital weighted subtraction and addition techniques. It was concluded that the microstructures of PEO are strongly perturbed by the PMMA's. Among these blends PEO microstructure in PEO/s-PMMA blends is most greatly influenced. It indicates that the blending is most preferred with s-PMMA than a- and i-PMMA. It can be explained on the basis of the molecular structure of PMMA's.