• 식물병연구
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• 제9권4호
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• pp.189-195
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• 2003

참외의 발효과를 유발하는 세균은 16S rDNA 염기분석에 의해 3 group으로 분류되었다. CM2105균주의 16S rDNA 염기서열은 Microbacterium phyllosphaerae의 염기서열과 99.6%의 높은 상동성을 나타냈고, M. holiorum과도 99.5%의 높은 상동성을 나타냈다. CM2101과 CM2121균주의 16S rDNA 염기서열은 "P. pavonaceae"와 각각 98.9%, 98.8, CM2126균주는 P. costantinii와 99.5%, P. grimontii와 99.0%의 높은 상동성을 나타냈다. CM21131균주의 16S rDNA 염기서열은 Enterobacter cloacae와 99.7%의 높은 상동성 나타냈다. 검정균주의 생리적, 생화학적 특성과 16S rDNA의 염기분석에 따라 CM2105 균주는 M. phyllosphaerae, CM2101, CM2121, CM2126 균주는 Pseudomonas spp., 그리고 CM2113 균주는 E. cloacae로 동정하였다.

• Journal of Microbiology
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• 제42권3호
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• pp.188-193
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• 2004

Xanthobacter flavus strain UE15 was isolated in wastewater obtained from the Ulsan industrial complex, Korea. This strain functions as a 1,2-dichloroethane (1,2-DCA) degrader, via a mechanism of hydrolytic dechlorination, under aerobic conditions. The UE15 strain was also capable of dechlorinating other chloroaliphatics such as 2-chloroacetic acid and 2-chloropropionic acid. The dhlA gene encoding 1,2-DCA dechlorinase was cloned from the genomic DNA of the UE15 strain, and its nucleotide sequence was determined to consist of 933 base pairs. The deduced amino acid sequence of the DhlA dechlorinase exhibited 100％ homology with the corresponding enzyme from X. autotrophicus GJ10, but only 27 to 29％ homology with the corresponding enzymes from Rhodococcus rhodochrous, Pseudomonas pavonaceae, and Mycobacterium sp. strain GP1, which all dechlorinate haloalkane compounds. The UE15 strain has an ORF1 (1,356 bp) downstream from the dhlA gene. The OFR1 shows 99％ amino acid sequence homology with the transposase reported from X. autotrophicus GJ10. The transposase gene was not found in the vicinity of the dhlA in the GJ10 strain, but rather beside the dhlB gene coding for haloacid dechlorinase. The dhlA and dhlB genes were confirmed to be located at separate chromosomal loci in the Xanthobacter flavus UE15 strain as well as in X. autotrophicus GJ10. The dhlA and transposase the UE15 strain were found to be parenthesized by a pair of insertion sequences, 181247, which were also found on both sides of the transposase gene in the GJ10 strain. This unique structure of the dhlA gene organization in X. flavus strain UE15 suggested that the dechlorinase gene, dhlA, is transferred with the help of the transposase gene.