Browse > Article
http://dx.doi.org/10.14405/kjvr.2021.61.e37

In vivo and in vitro efficacy of florfenicol, terbinafine, and mometasone furoate topical otic solution for the treatment of canine otitis externa  

Bae, Seulgi (Department of Veterinary Internal Medicine, College of Veterinary Medicine, Kyungpook National University)
Jin, Yunseok (Department of Veterinary Internal Medicine, College of Veterinary Medicine, Kyungpook National University)
Oh, Taeho (Department of Veterinary Internal Medicine, College of Veterinary Medicine, Kyungpook National University)
Publication Information
Korean Journal of Veterinary Research / v.61, no.4, 2021 , pp. 37.1-37.6 More about this Journal
Abstract
This study evaluated the efficacy of a new, single-dose otic solution combining florfenicol, terbinafine, and mometasone furoate for the treatment of canine otitis externa (OE) in vitro and in vivo. Forty-one client-owned dogs with OE were included in the study and divided into a treatment group that received the test solution and a negative control group that received a normal saline solution. On day 0, the dogs were treated either with the test or the control solution and evaluated over 30 days. Clinical efficacy was evaluated by clinical signs and cytological organism counts. In vitro antimicrobial susceptibility was evaluated by the minimum inhibitory concentration (MIC). After treatment with the test solution, clinical signs continuously decreased and cytological scores were significantly reduced. The results of MIC testing showed that the test solution was potent against the common pathogenic causes of canine OE. In this study, the most common causative pathogens were Staphylococcus pseudintermedius, Pseudomonas spp. and Malassezia pachydermatis. No issues related to safety were identified. Based on these results, this new ototopical drug can be used as first line treatment for canine OE.
Keywords
dogs; otitis externa; florfenicol; terbinafine; mometasone furoate;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Cannon M, Harford S, Davies J. A comparative study on the inhibitory actions of chloramphenicol, thiamphenicol and some fluorinated derivatives. J Antimicrob Chemother 1990;26:307-317.   DOI
2 Shin SJ, Kang SG, Nabin R, Kang ML, Yoo HS. Evaluation of the antimicrobial activity of florfenicol against bacteria isolated from bovine and porcine respiratory disease. Vet Microbiol 2005;106:73-77.   DOI
3 Guillot J, Bensignor E, Jankowski F, Seewald W, Chermette R, Steffan J. Comparative efficacies of oral ketoconazole and terbinafine for reducing Malassezia population sizes on the skin of Basset Hounds. Vet Dermatol 2003;14:153-157.   DOI
4 O Neill DG, Church DB, McGreevy PD, Thomson PC, Brodbelt DC. Prevalence of disorders recorded in dogs attending primary-care veterinary practices in England. PLoS One 2014;9:e90501.   DOI
5 Hill PB, Lo A, Eden CA, Huntley S, Morey V, Ramsey S, Richardson C, Smith DJ, Sutton C, Taylor MD, Thorpe E, Tidmarsh R, Williams V. Survey of the prevalence, diagnosis and treatment of dermatological conditions in small animals in general practice. Vet Rec 2006;158:533-539.   DOI
6 Hariharan H, Coles M, Poole D, Lund L, Page R. Update on antimicrobial susceptibilities of bacterial isolates from canine and feline otitis externa. Can Vet J 2006;47:253-255.
7 Nuttall T. Successful management of otitis externa. In Pract 2016;38:17-21.   DOI
8 Cole LK, Kwochka KW, Kowalski JJ, Hillier A. Microbial flora and antimicrobial susceptibility patterns of isolated pathogens from the horizontal ear canal and middle ear in dogs with otitis media. J Am Vet Med Assoc 1998;212:534-538.
9 De Martino L, Nocera FP, Mallardo K, Nizza S, Masturzo E, Fiorito F, Iovane G, Catalanotti P. An update on microbiological causes of canine otitis externa in Campania Region, Italy. Asian Pac J Trop Biomed 2016;6:384-389.   DOI
10 Hofbauer B, Leitner I, Ryder NS. In vitro susceptibility of Microsporum canis and other dermatophyte isolates from veterinary infections during therapy with terbinafine or griseofulvin. Med Mycol 2002;40:179-183.   DOI
11 Paterson S. The use of antibiotics and antimycotics in otitis. Companion Anim 2018;23:608-613.   DOI
12 Miller WH, Griffin CE, Campbell KL. Disease of Eyelids, Claws, anal Sacs, and Ears. In: Muller and Kirk's Small Animal Dermatology. 7th ed. pp. 724-773, Elsevier, St. Louis, 2013.
13 Blake J, Keil D, Kwochka K, Palma K, Schofield J. Evaluation of a single-administration ototopical treatment for canine otitis externa: a randomised trial. Vet Rec Open 2017;4:e000219.   DOI
14 Alexander BD. Reference Method for Broth Dilution Antifungal Susceptibility Testing of Yeasts. 4th ed. Clinical and Laboratory Standards Institute, Wayne, 2017.
15 Ganiere JP, Medaille C, Mangion C. Antimicrobial drug susceptibility of Staphylococcus intermedius clinical isolates from canine pyoderma. J Vet Med B Infect Dis Vet Public Health 2005;52:25-31.   DOI
16 Reeder CJ, Griffin CE, Polissar NL, Neradilek B, Armstrong RD. Comparative adrenocortical suppression in dogs with otitis externa following topical otic administration of four different glucocorticoid-containing medications. Vet Ther 2008;9:111-121.
17 Rosales MS, Marsella R, Kunkle G, Harris BL, Nicklin CF, Lopez J. Comparison of the clinical efficacy of oral terbinafine and ketoconazole combined with cephalexin in the treatment of Malassezia dermatitis in dogs: a pilot study. Vet Dermatol 2005;16:171-176.   DOI
18 Nuttall T, Bensignor E. A pilot study to develop an objective clinical score for canine otitis externa. Vet Dermatol 2014;25:530-e92.   DOI
19 Weinstein MP. Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically. 11th ed. Clinical and Laboratory Standards Institute, Wayne, 2018.