Browse > Article
http://dx.doi.org/10.14405/kjvr.2021.61.e26

Prevalence, species, and antimicrobial resistance of Acinetobacter in surgical practice and laboratory dog husbandry room environments  

Nakbubpa, Kulchai (Faculty of Veterinary Medicine, Rajamangala University of Technology Tawan-ok)
Janchawna, Ratchadaporn (Faculty of Veterinary Medicine, Rajamangala University of Technology Tawan-ok)
Thumchop, Wanatchaporn (Faculty of Veterinary Medicine, Rajamangala University of Technology Tawan-ok)
Panboonthong, Ailisa (Faculty of Veterinary Medicine, Rajamangala University of Technology Tawan-ok)
Pornsukarom, Suchawan (Faculty of Veterinary Medicine, Rajamangala University of Technology Tawan-ok)
Publication Information
Korean Journal of Veterinary Research / v.61, no.3, 2021 , pp. 26.1-26.8 More about this Journal
Abstract
Acinetobacter is a bacteria found in the environment and clinical specimens, causing nosocomial infection and antimicrobial resistance (AMR) threats. This study examined the prevalence, species, and AMR characteristics of Acinetobacter isolated from surgical practice and the laboratory dog husbandry room environments (n = 235) at Rajamangala University of Technology Tawan-ok veterinary hospital during 2018-2019. The prevalence of Acinetobacter in the laboratory dog husbandry room and veterinary belongings were 2.55% and 0.43%, respectively. Species determination was Acinetobacter hemolyticus (2.13%) and Acinetobacter baumannii (0.43%) from environments in the laboratory dog husbandry room, and Acinetobacter junii (0.43%) from the shoes used in the surgical practice room. AMR was observed in both study environments and the specimens sent to the Veterinary Diagnostic Center. These isolates had a high resistant percentage to amoxicillin-clavulanic acid (84.62%), sulfamethoxazole-trimethoprim (61.54%), and cephalexin (53.85%) but were susceptible to imipenem. Compared to the isolates recovered from the clinical specimens, most isolates derived from environments exhibited multidrug resistance and shared correlated resistance patterns. These results highlight the need for sanitization in the dog husbandry room. Furthermore, the AMR results can be used as a preliminary baseline for studying AMR Acinetobacter contamination in animals and their environments.
Keywords
Acinetobacter; environment; antimicrobial resistance; dog;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Black DM, Rankin SC, King LG. Antimicrobial therapy and aerobic bacteriologic culture patterns in canine intensive care unit patients: 74 dogs (January-June 2006). J Vet Emerg Crit Care (San Antonio) 2009;19:489-495.   DOI
2 Rosenbaum P, Aureden K, Cloughessy M, Goss L, Kassai M, Streed SA. Guide to the Elimination of Multidrug-Resistant Acinetobacter baumannii Transmission in Healthcare Settings. pp. 6-13, APIC, Washington, DC, 2010.
3 Muller S, Janssen T, Wieler LH. Multidrug resistant Acinetobacter baumannii in veterinary medicine: emergence of an underestimated pathogen? Berl Munch Tierarztl Wochenschr 2014;127:435-446.
4 Da Silva GJ, Domingues S. Insights on the horizontal gene transfer of carbapenemase determinants in the opportunistic pathogen Acinetobacter baumannii. Microorganisms 2016;4:29.   DOI
5 Karageorgopoulos DE, Falagas ME. Current control and treatment of multidrug- resistant Acinetobacter baumannii infections. Lancet Infect Dis 2008;8:751-762.   DOI
6 Otter JA, Yezli S, Salkeld JA, French GL. Evidence that contaminated surfaces contribute to the transmission of hospital pathogens and an overview of strategies to address contaminated surfaces in hospital settings. Am J Infect Control 2013;41(5 Suppl):S6-S11.
7 Belmonte O, Pailhories H, Kempf M, Gaultier MP, Lemarie C, Ramont C, Joly-Guillou ML, Eveillard M. High prevalence of closely-related Acinetobacter baumannii in pets according to a multicentre study in veterinary clinics, Reunion Island. Vet Microbiol 2014;170:446-450.   DOI
8 Chanawong A. Nonfermentative Gram Negative Bacilli. In: Sribenjalak P, Lulitanon A, eds. Clinical Bacteriology. 3rd ed. pp. 6-38, Department of Clinical Microbiology, Faculty of Medical Technology, Khon Kaen University, Khon Kaen, 2004.
9 Al Atrouni A, Joly-Guillou ML, Hamze M, Kempf M. Reservoirs of non-baumannii Acinetobacter species. Front Microbiol 2016;7:49.   DOI
10 Rajamohan G, Srinivasan VB, Gebreyes WA. Biocide-tolerant multidrug-resistant Acinetobacter baumannii clinical strains are associated with higher biofilm formation. J Hosp Infect 2009;73:287-289.   DOI
11 Vattanavanit V, Chayakul P. Acinetobacter infections in the intensive care unit. Songkla Med J 2013;2:91-100.
12 NNIS System. National Nosocomial Infections Surveillance (NNIS) system report, data summary from January 1990-May 1999, issued June 1999. A report from the NNIS System. Am J Infect Control 1999;27:520-532.   DOI
13 Saelao S, Utiswannakul A. Therapy for patient with multidrug-resistant Acinetobacter baumannii. J Health Res 2008;22:131-136.
14 Uwingabiye J, Frikh M, Lemnouer A, Bssaibis F, Belefquih B, Maleb A, Dahraoui S, Belyamani L, Bait A, Haimeur C, Louzi L, Ibrahimi A, Elouennass M. Acinetobacter infections prevalence and frequency of the antibiotics resistance: comparative study of intensive care units versus other hospital units. Pan Afr Med J 2016;23:191.
15 Chastre J. Infections due to Acinetobacter baumannii in the ICU. Semin Respir Crit Care Med 2003;24:69-78.   DOI
16 Bergogne-Berezin E. The increasing significance of outbreaks of Acinetobacter spp: the need for control and new agents. J Hosp Infect 1995;30 Suppl:441-452.   DOI
17 Villegas MV, Hartstein AI. Acinetobacter outbreaks, 1977-2000. Infect Control Hosp Epidemiol 2003;24:284-295.   DOI
18 Zordan S, Prenger-Berninghoff E, Weiss R, van der Reijden T, van den Broek P, Baljer G, Dijkshoorn L. Multidrug-resistant Acinetobacter baumannii in veterinary clinics, Germany. Emerg Infect Dis 2011;17:1751-1754.   DOI
19 Maboni G, Seguel M, Lorton A, Sanchez S. Antimicrobial resistance patterns of Acinetobacter spp. of animal origin reveal high rate of multidrug resistance. Vet Microbiol 2020;245:108702.   DOI
20 van der Kolk JH, Endimiani A, Graubner C, Gerber V, Perreten V. Acinetobacter in veterinary medicine, with an emphasis on Acinetobacter baumannii. J Glob Antimicrob Resist 2019;16:59-71.   DOI
21 Nocera FP, Attili AR, De Martino L. Acinetobacter baumannii: its clinical significance in human and veterinary medicine. Pathogens 2021;10:127.   DOI
22 Rafei R, Hamze M, Pailhories H, Eveillard M, Marsollier L, Joly-Guillou ML, Dabboussi F, Kempf M. Extrahuman epidemiology of Acinetobacter baumannii in Lebanon. Appl Environ Microbiol 2015;81:2359-2367.   DOI
23 Abo-Zed A, Yassin M, Phan T. Acinetobacter junii as a rare pathogen of urinary tract infection. Urol Case Rep 2020;32:101209.   DOI
24 Bergogne-Berezin E, Towner KJ. Acinetobacter spp. as nosocomial pathogens: microbiological, clinical, and epidemiological features. Clin Microbiol Rev 1996;9:148-165.   DOI
25 Bouvet PJ, Grimont PA. Identification and biotyping of clinical isolates of Acinetobacter. Ann Inst Pasteur Microbiol 1987;138:569-578.   DOI
26 Constantiniu S, Romaniuc A, Iancu LS, Filimon R, Tarasi I. Cultural and biochemical characteristics of Acinetobacter spp. strains isolated from hospital units. J Prev Med 2004;12:35-42.
27 Lone R, Shah A, Kadri SM, Lone S, Faisal S. Nosocomial multi-drug-resistant Acinetobacter infections-clinical findings, risk factors and demographic characteristics. Bangladesh J Med Microbiol 2009;3:34-38.   DOI
28 Doughari HJ, Ndakidemi PA, Human IS, Benade S. The ecology, biology and pathogenesis of Acinetobacter spp.: an overview. Microbes Environ 2011;26:101-112.   DOI
29 Wayne PA. Clinical and Laboratory Standards Institute. Performance Standards for Antimicrobial Susceptibility Testing; Twenty-Second Informational Supplement: CLSI document M100-S22. pp. 66-74, Clinical and Laboratory Standards Institute, Pennsylvania, 2011.
30 Almasaudi SB. Acinetobacter spp. as nosocomial pathogens: epidemiology and resistance features. Saudi J Biol Sci 2018;25:586-596.   DOI
31 Leclercq R, Canton R, Brown DF, Giske CG, Heisig P, MacGowan AP, Mouton JW, Nordmann P, Rodloff AC, Rossolini GM, Soussy CJ, Steinbakk M, Winstanley TG, Kahlmeter G. EUCAST expert rules in antimicrobial susceptibility testing. Clin Microbiol Infect 2013;19:141-160.   DOI
32 Bansal G, Allen-McFarlane R, Eribo B. Antibiotic susceptibility, clonality, and molecular characterization of carbapenem-resistant clinical isolates of Acinetobacter baumannii from Washington DC. Int J Microbiol 2020;2020:2120159.
33 Jung J, Park W. Acinetobacter species as model microorganisms in environmental microbiology: current state and perspectives. Appl Microbiol Biotechnol 2015;99:2533-2548.   DOI
34 National Antimicrobial Resistance Surveillance of Thailand. Antimicrobial Resistance Microbes Situation in 2000-2020. pp. 9, 11-13, National Institute of Health of Thailand, Department of Medical Sciences, Nonthaburi, 2020.
35 Rebic V, Masic N, Teskeredzic S, Aljicevic M, Abduzaimovic A, Rebic D. The importance of Acinetobacter species in the hospital environment. Med Arch 2018;72:325-329.   DOI
36 Castro-Jaimes S, Bello-Lopez E, Velazquez-Acosta C, Vol-kow-Fernandez P, Lozano-Zarain P, Castillo-Ramirez S, Cevallos MA. Chromosome architecture and gene content of the emergent pathogen Acinetobacter haemolyticus. Front Microbiol 2020;11:926.   DOI
37 Gupta N, Gandham N, Jadhav S, Mishra RN. Isolation and identification of Acinetobacter species with special reference to antibiotic resistance. J Nat Sci Biol Med 2015;6:159-162.   DOI