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http://dx.doi.org/10.14405/kjvr.2014.54.2.75

Evaluation of the immunogenicity of Bordetella bronchiseptica, a vaccine antigen  

Woo, Su-Han (Laboratory of Veterinary Pharmacology, College of Veterinary Medicine, Jeju National University)
Moon, Sun-Young (Laboratory of Veterinary Pharmacology, College of Veterinary Medicine, Jeju National University)
Byon, Yun-Young (Laboratory of Veterinary Pharmacology, College of Veterinary Medicine, Jeju National University)
Joo, Hong-Gu (Laboratory of Veterinary Pharmacology, College of Veterinary Medicine, Jeju National University)
Publication Information
Korean Journal of Veterinary Research / v.54, no.2, 2014 , pp. 75-79 More about this Journal
Abstract
Bordetella (B.) bronchiseptica is a causative agent of swine atrophic rhinitis that promotes colonization of the mucous membrane of the swine nasal cavity by Pasteurella (P.) multocida. Mixed infection with B. bronchiseptica and P. multocida leads to growth inhibition of pigs, resulting in significant economic loss. There are many commercial vaccines for atrophic rhinitis, including B. bronchiseptica as a killed vaccine antigen (Ag). However, the immunogenicity of killed B. bronchiseptica Ag has not yet been elucidated; therefore, this study was conducted to investigate the immunogenicity of killed B. bronchiseptica Ag and the type of immune response it induces. In vitro assays using mouse spleen cells and flow cytometry revealed that B. bronchiseptica Ag induced high proliferation capability of lymphocytes, especially B lymphocytes, and the proliferating cells showed a significant response to interleukin (IL)-2. B. bronchiseptica Ag also enhanced the production of IL-12, a representative cytokine for cell-mediated immunity. In vivo experiments using mice showed that the injection of B. bronchiseptica Ag markedly induced Ag-specific antibody. Taken together, these results indicate that B. bronchiseptica Ag has high immunogenicity by itself.
Keywords
Bordetella bronchiseptica; immunogenicity; spleen cells;
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Times Cited By KSCI : 2  (Citation Analysis)
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