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http://dx.doi.org/10.14405/kjvr.2013.53.2.095

Comparative evaluation of the murine immune responses to Salmonella enterica serovars Enteritidis, Gallinarum and Typhimurium infection  

Kim, Kiju (College of Veterinary Medicine & Institute of Veterinary Science, Kangwon National University)
Kim, Dooree (College of Veterinary Medicine & Institute of Veterinary Science, Kangwon National University)
Sun, Jisun (College of Veterinary Medicine & Institute of Veterinary Science, Kangwon National University)
Park, Soyeon (College of Veterinary Medicine & Institute of Veterinary Science, Kangwon National University)
Cho, Youngjae (College of Veterinary Medicine & Institute of Veterinary Science, Kangwon National University)
Ko, Hyun-Jeong (College of Pharmacy, Kangwon National University)
Joo, Hong-Gu (College of Veterinary Medicine, Jeju National University)
Hahn, Tae-Wook (College of Veterinary Medicine & Institute of Veterinary Science, Kangwon National University)
Publication Information
Korean Journal of Veterinary Research / v.53, no.2, 2013 , pp. 95-101 More about this Journal
Abstract
The study was carried out to evaluate and compare the immune responses in mice experimentally infected with either wild-type or isogenic mutants of Salmonella enterica serovars Enteritidis (SE), Salmonella Typhimurium (ST) and Gallinarum (SG). The mutant strains were constructed by allelic replacement of some virulence-associated genes in the wild-type strains. Seven-week-old female BALB/c mice were orally or intraperitoneally inoculated by injecting bacterial suspension. To evaluate the immune responses, enzyme-linked immunosorbent assay (ELISA) and enzyme-linked immunospot (ELISPOT) assay were conducted with serum and fecal samples. As a result, the mice group infected orally with the SE mutant strain showed the highest level of specific IgA-secreting splenocytes, compared to the other groups. The peritoneally injected groups showed the greater levels of IgG1 than the orally injected groups, which was in a good agreement with the previous studies. In addition, the mutant infected groups had the similar secretion levels of antibodies with the wild-type infected groups. These results demonstrated that the SE mutant strain elicited humoral immune response as much as wild-type, implying that it can be useful as a delivery vehicle as well as a candidate of a live attenuated vaccine.
Keywords
humoral immunity; mucosal immunity; Salmonella;
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