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Proteome analysis between diverse phenotypes of Salmonella enterica subspecies enterica serovar Typhimurium (S. Typhimurium)  

Shin, Gee-Wook (College of Veterinary Medicine, Chonbuk National University)
Cha, In-Seok (College of Veterinary Medicine, Gyeongsang National University)
Lee, Woo-Won (Busan Metropolitan City Institute of Health and Environmental)
Nho, Seong-Won (College of Veterinary Medicine, Gyeongsang National University)
Park, Seong-Bin (College of Veterinary Medicine, Gyeongsang National University)
Jang, Ho-Bin (College of Veterinary Medicine, Gyeongsang National University)
Kim, Yong-Hwan (College of Veterinary Medicine, Gyeongsang National University)
Jung, Tae-Sung (College of Veterinary Medicine, Gyeongsang National University)
Publication Information
Korean Journal of Veterinary Research / v.50, no.4, 2010 , pp. 285-295 More about this Journal
Abstract
Protein expression patterns in Salmonella enterica subspecies enterica serovar Typhimurium (S. Typhimurium) strains with diverse phenotypes, such as phage type, antibiotic resistance pattern and plasmid profiles were examined. For detailed analysis of proteins expressed by different S. Typhimurium strains, protein fractions were divided into detergent-rich phase (DP) and aqueous phase (AP) using triton X-114 detergent. The two phases were subjected to two-dimensional gel electrophoresis (2-DE), followed by protein identification using peptide mass fingerprinting (PMF). In the results, PMF showed that DP fractions consisted mainly of outer membrane proteins, whereas the AP fractions included cytosolic proteins. Comparison of 2-DE profiles of DP did not show any distinct protein spots which could be correlated with phage type, antibiotic resistance pattern or plasmid profile. However, comparisons of 2-DE profiles of the AP revealed differences in the protein spots, which could be correlated with the plasmid profile and phage types. Among these protein spots, flagellin was specific for strains containing a 90 kb plasmid. Compared to DT193 phage type, three protein spots in the range of pI 5.0-5.5 and MW 8-15 kDa of AP 2-DE profiles were absent in the DT104 phage types. Additionally, a protein spot with PI in the range of 4.5-5.0 and molecular weight (MW) between 51-69 kDa was specific for phage type DT104, while a protein spot with pI in the range of 4.0-4.8 and MW between 18-20 kDa was specific for DT193 phage type. These protein spots may be useful for discriminating phage types of S. Typhimurium.
Keywords
OMP; phage type; plasmid; proteome; Salmonella enterica serovar Typhimurium; Triton X-114;
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