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Characterization of Yersinia ruckeri isolated from the farm-cultured eel Anguilla japonica in Korea  

Joh, Seong Joon (Laboratory of Aquatic Animal Diseases, Department of Animal Health, National Veterinary Research and Quarantine Service)
Kweon, Chang Hee (Laboratory of Aquatic Animal Diseases, Department of Animal Health, National Veterinary Research and Quarantine Service)
Kim, Min Jeong (Laboratory of Aquatic Animal Diseases, Department of Animal Health, National Veterinary Research and Quarantine Service)
Kang, Min Su (Laboratory of Aquatic Animal Diseases, Department of Animal Health, National Veterinary Research and Quarantine Service)
Jang, Hwan (Laboratory of Aquatic Animal Diseases, Department of Animal Health, National Veterinary Research and Quarantine Service)
Kwon, Jun Hun (Laboratory of Aquatic Animal Diseases, Department of Animal Health, National Veterinary Research and Quarantine Service)
Publication Information
Korean Journal of Veterinary Research / v.50, no.1, 2010 , pp. 29-35 More about this Journal
Abstract
Yersinia (Y.) ruckeri has been recognized as a serious bacterial pathogen to several kinds of fish, including rainbow trout. However, there are no reports about the characteristics and pathogenicity of Y. ruckeri isolated from farm-cultured eels. In this study, we isolated and characterized Y. ruckeri from the farm-cultured eel Anguilla japonica in Korea. We investigated the phenotypic and genotypic characteristics of Y. ruckeri and tested the virulence of Y. ruckeri isolates on experimentally infected eels. Examination of the flagellar morphology of Y. ruckeri by electron microscopy showed peritrichous flagella in its cell body. Biochemical reaction studies showed overall identical profiles between the isolates and the reference strain of Y. ruckeri in API 20E and API ZYM tests. We sequenced the 16S rRNA of the Y. ruckeri (1,505 bp) for the genotypic characterization (National Center for Biotechnology Information accession number EU401667). Comparison of the 16S rRNA sequences with previously reported Y. ruckeri strains revealed similar phylogenetic relationships. In the virulence assay of the Y. ruckeri on eels, the eels exhibited listlessness, but Y. ruckeri was reisolated from those of the gills and kidneys.
Keywords
characteristics; eel; virulence; Yersinia ruckeri;
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1 Berc A, Petrinec Z, Matasin Z, Kozari Z. Yersinia ruckeri septicaemia in experimentally infected carp (Cyprinus carpio L.) fingerlings. Acta Vet Hung 1999, 47, 161-172   DOI   PUBMED
2 Furones MD, Rodgers CJ, Munn CB. Yersinia ruckeri, the causal agent of enteric redmouth disease (ERM) in fish. Annu Rev Fish Dis 1993, 3, 105-125   DOI   ScienceOn
3 Kim W, Song MO, Song W, Kim KJ, Chung SI, Choi CS, Park YH. Comparison of 16S rDNA analysis and rep-PCR genomic fingerprinting for molecular identification of Yersinia pseudotuberculosis. Antonie Van Leeuwenhoek 2003, 83, 125-133   DOI   ScienceOn
4 Tobback E, Decostere A, Hermans K, Haesebrouck F, Chiers K. Yersinia ruckeri infections in salmonid fish. J Fish Dis 2007, 30, 257-268   DOI   ScienceOn
5 Frerichs GN. Isolation and identification of fish bacterial pathogens. In: Inglis V, Roberts RJ, Bromage NR (eds.). Bacterial Diseases of Fish. pp. 270-272, Blackwell Scientific, Oxford, 1993
6 Buller NB. Bacteria from Fish and Other Aquatic Animals: A Practical Identification Manual. p. 74, CABI Publishing, Cambridge, 2004
7 García-López I, Otero A, García-López ML, Santos JA. Molecular and phenotypic characterization of nonmotile Gram-negative bacteria associated with spoilage of freshwater fish. J Appl Microbiol 2004, 96, 878-886   DOI   ScienceOn
8 Romalde JL, Toranzo AE. Evaluation of the API 20E system for the routine diagnosis of the enteric redmouth disease. Bull Eur Assoc Fish Pathol 1991, 11, 147-149
9 Stevenson RMW, Daly JG. Biochemical and serological characteristics of Ontario isolates of Yersinia ruckeri. Can J Fish Aquat Sci 1982, 39, 870-876   DOI
10 Bullock GL, Stuckey HM, Shotts EB Jr. Enteric redmouth bacterium: comparison of isolates from different geographic areas. J Fish Dis 1978, 1, 351-356   DOI
11 Ross AJ, Rucker RR, Ewing WH. Description of a bacterium associated with redmouth disease of rainbow trout (Salmo gairdneri). Can J Microbiol 1966, 12, 763-770   DOI   ScienceOn
12 Austin DA, Robertson PAW, Austin B. Recovery of a new biogroup of Yersinia ruckeri from diseased rainbow trout (Oncorhynchus mykiss, Walbaum). Syst Appl Microbiol 2003, 26, 127-131   DOI   ScienceOn
13 Coquet L, Cosette P, Quillet L, Petit F, Junter GA, Jouenne T. Occurrence and phenotypic characterization of Yersinia ruckeri strains with biofilmforming capacity in a rainbow trout farm. Appl Environ Microbiol 2002, 68, 470-475   DOI   ScienceOn
14 Sambrook J, Russell DW. Molecular Cloning: A Laboratory Manual. 3rd ed. pp. 1.35-1.37, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, 2001
15 Fouz B, Zarza C, Amaro C. First description of nonmotile Yersinia ruckeri serovar I strains causing disease in rainbow trout, Oncorhynchus mykiss (Walbaum), cultured in Spain. J Fish Dis 2006, 29, 339-346   DOI   ScienceOn
16 Danley ML, Goodwin AE, Killian HS. Epizootics in farm-raised channel catfish, Ictalurus punctatus (Rafinesque), caused by the enteric redmouth bacterium Yersinia ruckeri. J Fish Dis 1999, 22, 451-456   DOI   ScienceOn
17 Llewellyn LC. A bacterium with similarities to the redmouth bacterium and Serratia liquefaciens (Grimes and Hennerty) causing mortalities in hatchery reared salmonids in Australia. J Fish Dis 1980, 3, 29-39   DOI
18 Rucker RR. Redmouth disease of rainbow trout (Salmo gairdneri). Bull Off Int Epizoot 1966, 65, 825-830   PUBMED
19 De Grandis SD, Krell PJ, Flett DE, Stevenson RMW. Deoxyribonucleic acid relatedness of serovars of Yersinia ruckeri, the enteric redmouth bacterium. Int J Syst Bacteriol 1988, 38, 49-55   DOI
20 Davies RL, Frerichs GN. Morphological and biochemical differences among isolates of Yersinia ruckeri obtained from wide geographical areas. J Fish Dis 1989, 12, 357-365   DOI
21 Clinical and Laboratory Standards Institute. Performance Standards for Antimicrobial Disk and Dilution Susceptibility Tests for Bacteria Isolated from Animals; Approved Standard - 6th ed. M2-AP. Clinical and Laboratory Standards Institute, Wayne, 2006
22 Santos Y, Romalde JL, Bandin I, Magarinos B, Nunez S, Barja JL, Toranzo AE. Usefulness of the API-20E system for the identification of bacterial fish pathogens. Aquaculture 1993, 116, 111-120   DOI   ScienceOn
23 Ewing WH, Ross AJ, Brenner DJ, Fanning GR. Yersinia ruckeri sp. nov., the redmouth (RM) bacterium. Int J Syst Bacteriol 1978, 28, 37-44   DOI
24 Kotetishvili M, Kreger A, Wauters G, Morris JG Jr, Sulakvelidze A, Stine OC. Multilocus sequence typing for studying genetic relationships among Yersinia species. J Clin Microbiol 2005, 43, 2674-2684   DOI   ScienceOn
25 Cipriano RC, Schill WB, Pyle SW, Horner R. An epizootic in Chinook salmon (Oncorhynchus tshawytscha) caused by a sorbitol-positive serovar 2 strain of Yersinia ruckeri. J Wildl Dis 1986, 22, 488-492   DOI   PUBMED