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Random amplification of polymorphic DNA typing of Listeria monocytogenes isolates from animal products  

Lee, Chul-Hyun (National Veterinary Research and Quarantine Service)
Son, Won-Geun (College of Veterinary Medicine and Veterinary Medical Research Institute)
Publication Information
Korean Journal of Veterinary Research / v.49, no.4, 2009 , pp. 319-328 More about this Journal
Abstract
This study investigated the epidemiology of Listeria (L.) monocytogenes, a food-borne pathogen. The epidemiology of food-borne pathogens is of great importance for clarifying bacterial origin and preventing bacterial contamination and infection. This work examined 68 L. monocytogenes strains, including 11 reference strains and 57 isolates from imported US beef, domestic meats (beef, pork, chicken meat), raw milk, and milk plants. The random amplified polymorphic DNA (RAPD) techniques were optimized to develop a standard molecular epidemiological analysis of L. monocytogenes. There was great genetic variability among the isolates, which produced 24 and 34 RAPD patterns with primer HLWL85 and HLWL74, respectively. The discriminatory power of the RAPD methods with HLWL85 and HLWL74 primer were very high (DI = 0.957; S ${\geq}$ 80%, S ${\geq}$ 95%). Some RAPD types were specific to origin. A few RAPD types were specific for L. monocytogenes strains belonging to a particular serotype. Using the HLWL85 primer, the strains isolated from milk plants could be distinguished from the other strains. And using the HLWL74 primer, the strains isolated from imported beef (US) could be distinguished completely from the other strains.
Keywords
Listeria monocytogenes; molecular epidemiology; RAPD typing;
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1 Bille J, Rocourt J. WHO International Multicenter Listeria monocytogenes subtyping study-rationale and set-up of the study. Int J Food Microbiol 1996, 32, 251-262   DOI   ScienceOn
2 Fonnesbech Vogel B, Huss HH, Ojeniyi B, Ahrens P, Gram L. Elucidation of Listeria monocytogenes contamination routes in cold-smoked salmon processing plants detected by DNA-based typing methods. Appl Environ Microbiol 2001, 67, 2586-2595   DOI   PUBMED   ScienceOn
3 Vogel BF, Jørgensen LV, Ojeniyi B, Huss HH, Gram L. Diversity of Listeria monocytogenes isolates from cold-smoked salmon produced in different smokehouses as assessed by Random Amplified Polymorphic DNA analyses. Int J Food Microbiol 2001, 65, 83-92   DOI   PUBMED   ScienceOn
4 Zhang W, Jayarao BM, Knabel SJ. Multi-virulencelocus sequence typing of Listeria monocytogenes. Appl Environ Microbiol 2004, 70, 913-920   DOI   ScienceOn
5 Micheli MR, Bova R, Pascale E, D'Ambrosio E. Reproducible DNA fingerprinting with the random amplified polymorphic DNA (RAPD) method. Nucleic Acids Res 1994, 22, 1921-1922   DOI   ScienceOn
6 Quintaes BR, Leal NC, Reis EM, Hofer E. Optimization of randomly amplified polymorphic DNA-polymerase chain reaction for molecular typing of Salmonella enterica serovar Typhi. Rev Soc Bras Med Trop 2004, 37, 143-147   DOI   ScienceOn
7 Byun SK, Jung SC, Yoo HS. Random amplification of polymorphic DNA typing of Listeria monocytogenes isolated from meat. Int J Food Microbiol 2001, 69, 227-235   DOI   ScienceOn
8 Inoue S, Katagiri K, Terao M, Maruyama T. RAPD- and actA gene-typing of Listeria monocytogenes isolates of human listeriosis, the intestinal contents of cows and beef. Microbiol Immunol 2001, 45, 127-133   DOI   PUBMED
9 Betancor L, Schelotto F, Martinez A, Pereira M, Algorta G, Rodríguez MA, Vignoli R, Chabalgoity JA. Random amplified polymorphic DNA and phenotyping analysis of Salmonella enterica serovar Enteritidis isolates collected from humans and poultry in Uruguay from 1995 to 2002. J Clin Microbiol 2004, 42, 1155-1162   DOI   ScienceOn
10 Aguado V, Vitas AI, Garc$\a-Jalón I. Characterization of Listeria monocytogenes and Listeria innocua from a vegetable processing plant by RAPD and REA. Int J Food Microbiol 2004, 90, 341-347   DOI   ScienceOn
11 Hunter PR, Gaston MA. Numerical index of the discriminatory ability of typing systems: an application of Simpson's index of diversity. J Clin Microbiol 1988, 26, 2465-2466   PUBMED
12 Seeliger HPR, Hohne K. Serotyping of Listeria monocytogenes and related species. In: Bergan T, Norris JR (eds.). Methods in Microbiology. pp. 31–49, Academic Press, London, 1979
13 Bannerman E, Boerlin P, Bille J. Typing of Listeria monocytogenes by monocin and phage receptors. Int J Food Microbiol 1996, 31, 245-262   DOI   PUBMED   ScienceOn
14 Penner GA, Bush A, Wise R, Kim W, Domier L,M Kasha K, Laroche A, Scoles G, Molnar SJ, Fedak G. Reproducibility of random amplified polymorphic DNA (RAPD) analysis among laboratories. PCR Methods Appl 1993, 2, 341-345   DOI   PUBMED
15 Wernars K, Boerlin P, Audurier A, Russell EG, Curtis GD, Herman L, van der Mee-Marquet N. The WHO multicenter study on Listeria monocytogenes subtyping: random amplification of polymorphic DNA (RAPD). Int J Food Microbiol 1996, 32, 325-341   DOI   ScienceOn
16 Tran HL, Kathariou S. Restriction fragment length polymorphisms detected with novel DNA probes differentiate among diverse lineages of serogroup 4 Listeria monocytogenes and identify four distinct lineages in serotype 4b. Appl Environ Microbiol 2002, 68, 59-64   DOI   ScienceOn
17 Lawrence LM, Gilmour A. Characterization of Listeria monocytogenes isolated from poultry products and from the poultry-processing environment by random amplification of polymorphic DNA and multilocus enzyme electrophoresis. Appl Environ Microbiol 1995, 61, 2139-2144   PUBMED
18 Leal NC, Sobreira M, Leal-Balbino TC, de Almeida AM, de Silva MJ, Mello DM, Seki LM, Hofer E. Evaluation of a RAPD-based typing scheme in a molecular epidemiology study of Vibrio cholerae O1, Brazil. J Appl Microbiol 2004, 96, 447-454   DOI   ScienceOn
19 Martinez I, R$\phi$rvik LM, Brox V, Lassen J, Seppola M, Gram L, Fonnesbech-Vogel B. Genetic variability among isolates of Listeria monocytogenes from food products, clinical samples and processing environments, estimated by RAPD typing. Int J Food Microbiol 2003, 84, 285-297   DOI   ScienceOn
20 Boerlin P, Bannerman E, Ischer F, Rocourt J, Bille J. Typing Listeria monocytogenes: a comparison of random amplification of polymorphic DNA with 5 other methods. Res Microbiol 1995, 146, 35-49   DOI   ScienceOn
21 Giovannacci I, Ragimbeau C, Queguiner S, Salvat G, Vendeuvre JL, Carlier V, Ermel G. Listeria monocytogenes in pork slaughtering and cutting plants. Use of RAPD, PFGE and PCR-REA for tracing and molecular epidemiology. Int J Food Microbiol 1999, 53, 127-140   DOI   ScienceOn
22 Mazurier SI, Wernars K. Typing of Listeria strains by random amplification of polymorphic DNA. Res Microbiol 1992, 143, 499-505   DOI   ScienceOn
23 Mereghetti L, Lanotte P, Savoye-Marczuk V, Marquet- Van Der Mee N, Audurier A, Quentin R. Combined ribotyping and random multiprimer DNA analysis to probe the population structure of Listeria monocytogenes. Appl Environ Microbiol 2002, 68, 2849-2857   DOI   ScienceOn
24 Delgado da Silva MC, Destro MT, Hofer E, Tibana A. Characterization and evaluation of some virulence markers of Listeria monocytogenes strains isolated from Brazilian cheeses using molecular, biochemical and serotyping techniques. Int J Food Microbiol 2001, 63, 275-280   DOI   ScienceOn
25 Martinez I, Friis TJ, Seppola M. Requirements for the application of protein sodium dodecyl sulfatepolyacrylamide gel electrophoresis and randomly amplified polymorphic DNA analyses to product speciation. Electrophoresis 2001, 22, 1526-1533   DOI   ScienceOn
26 Aguado V, Vitas AI, Garc$\a-Jalon I. Random amplified polymorphic DNA typing applied to the study of crosscontamination by Listeria monocytogenes in processed food products. J Food Prot 2001, 64, 716-720   DOI   PUBMED
27 Wagner M, Maderner A, Brandl E. Development of a multiple primer RAPD assay as a tool for phylogenetic analysis in Listeria spp. strains isolated from milkproduct associated epidemics, sporadic cases of listeriosis and dairy environments. Int J Food Microbiol 1999, 52, 29-37   DOI   ScienceOn
28 Lopes VC, Velayudhan BT, Halvorson DA, Lauer DC, Gast RK, Nagaraja KV. Comparison of methods for differentiation of Salmonella enterica serovar Enteritidis phage type 4 isolates. Am J Vet Res 2004, 65, 538-543   DOI   ScienceOn