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Regulatory roles of NKT cells in Anaplasma phagocytophilum infection  

Choi, Kyoung-Seong (College of Ecology and Environmental Sciences, Kyungpook National University)
Chae, Joon-Seok (College of Veterinary Medicine and BK21 Program for Veterinary Science, Seoul National University)
Publication Information
Korean Journal of Veterinary Research / v.49, no.2, 2009 , pp. 167-172 More about this Journal
Abstract
Human granulocytic anaplasmosis (HGA) is caused by the obligate intracellular bacterium Anaplasma (A.) phagocytophilum. Natural killer T (NKT) cells are key players in host defense against various microbial infections. We investigated the role of NKT cells in immune response to A. phagocytophilum infection using NKT-knockout ($J\alpha$18-/-) mice. $J\alpha$18-/- and wild-type (WT) mice were infected with low-passage A. phagocytophilum and assayed for hepatic histopathology and cytokine production during 7 days post-infection. Compared to WT controls, the infected $J\alpha$18 -/- mice had much less histopathologic lesions and less apoptosis through day 7, and lower concentrations of ${IFN\gamma}$ and IL- 12, but not of IL-10. This result suggests that NKT cells are major components in the pathogenesis of HGA.
Keywords
Anaplasma phagocytophilum; human granulocytic anaplasmosis; NKT cells; NKT-knockout mice;
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