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Occurrence of aflatoxin $M_1$ in milk determined by HPLC with derivatization method in Korea (1999-2000)  

Kang, Hwan Goo (National Veterinary Research and Quarantine Service)
Cho, Joon Hyoung (National Veterinary Research and Quarantine Service)
Publication Information
Korean Journal of Veterinary Research / v.47, no.4, 2007 , pp. 389-393 More about this Journal
Abstract
In this study, the levels of aflatoxin $M_1$ ($AFM_1$) in milk were determined by HPLC with derivatization method. Milk samples were purified using $C_{18}$ disposable cartridge followed by derivatization with trifluoroacetic acid and analysed using HPLC with fluorescence detection. The recoveries of AFM1 from milk samples added $AFM_1$ at a level of 0.025~0.1 ng/ml were 94.7~98.0% with detection limit of 0.009 ng/ml. The amounts of $AFM_1$ were determined below 0.05 ng/ml for all tested samples of commercial milk collected in 1999 and 2000.
Keywords
Aflatoxin $M_1$; HPLC; milk;
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1 Bakirci I. A study on the occurrence of aflatoxin $M_{1}$ in milk and milk products produced in Van province of Turkey. Food Control 2001, 12, 47-51   DOI   ScienceOn
2 Dominguez L, Blanco JL, Gomez-Lucia E, Rodriguez EF, Suarez G Determination of aflatoxin $M_{1}$ in milk and milk products contaminated low levels. J Assoc Off Anal Chem 1987, 70, 470-472
3 Lee HS, Cho BH, Son SW, Kang HG, Park JM. Rapid extraction and detection of aflatoxin $M_{1}$ in raw milk by high performance liquid chromatography. RDA J Veterinary Sci 1998, 40, 55-59
4 MAFF UK. Survey of aflatoxin $M_{1}$ in retail milk and milk products. Food surveillance information sheet. 64, 1995
5 van Egmond HP. Aflatoxin $M_{1}$ : occurrence, toxicity, regulation. In: Mycotoxin in Dairy Products. pp. 57-95, Elsevier, New York, 1989
6 WHO, CODEX. Report of the 32nd session of the codex committee on food additives and contaminants. 20-24, March, 2000. Codex, FAO, Rome, 2000
7 EL-Nezami HS, Nicoletti G, Neal GE, Donohue DC, Ahokas JT. Aflatoxin $M_{1}$ in Human breast milk samples from Victoria, Australia and Thailand. Food Chem Toxicol 1995, 33, 173-179   DOI   ScienceOn
8 van Egmond HP. Monograph on residues and contaminants in milk and milk products, chapter 8 mycotoxins. In: International Dairy Federation Special Issue 9101. pp. 156-166, International Dairy Federation, Brussels, 1997
9 Faletto MB, Koser PL, Battula N, Townsend GK, Maccubbin AE, Gelbion HV, Gurtoo HL. Cytochrome P3-450 cDNA encodes aflatoxin B1-4-hydroxylase. J Biol Chem 1988, 263, 12187-12189
10 Stubblefield RD. Optimum conditions for formation of aflatoxin $M_{1}$-trifluoroacetic acid derivative. J Assoc Off Anal Chem 1987, 70, 1047-1049
11 D'Melllo JPF, Macdonald AMC. Mycotoxins. Ani Feed Sci Technol 1997, 69, 155-166   DOI   ScienceOn
12 Kim EK, Shon DH, Ryu D, Park JW, Hwang HJ, Kim YB. Occurrence of aflatoxin $M_{1}$ in Korean dairy products determined by ELISA and HPLC. Food Addit Contam 2000, 17, 59-64   DOI
13 van Egmond HP, WagstafTe PJ. The certification of aflatoxin $M_{1}$ in 3 milk powder samples. Report EUR 10412. Commission of the European Communities, Brussel, 1988