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Evaluation of the extract from lymph nodes for Salmonella monitoring in pig herds  

Jung, Byeong-Yeal (National Veterinary Research and Quarantine Service)
Choo, Ji-Hoon (College of Veterinary Medicine, Kyungpook National University)
Kim, Ji-Hun (College of Veterinary Medicine, Kyungpook National University)
Jung, Jae-Yun (College of Veterinary Medicine, Kyungpook National University)
Publication Information
Korean Journal of Veterinary Research / v.46, no.2, 2006 , pp. 119-125 More about this Journal
Abstract
The objective of this study was to investigate the use of extract from mesenteric lymph nodes as an alternative to serum for ELISA to detect Salmonella antibodies in slaughter pigs. Among 324 slaughter pigs, 65 (20.1 %) were positive in the serum ELISA and 76 (23.5%) were positive in the ELISA with extract from lymph nodes. A total of 24 (7.4%) Salmonella representing 6 serotypes were isolated from mesenteric lymph nodes and 35 (10.8%) Salmonella belonging to 2 serotypes were also recovered from cecal contents of slaughter pig samples, respectively. The most prevalent serogroup was B (55.9% of isolates) and serotype was Typhimurium (52.5% of isolates). In the comparison of the results of between the serum ELISA and Salmonella isolation, kappa value was 0.28 with mesenteric lymph nodes and 0.37 with cecal contents, respectively. However, the extract ELISA had sensitivity of 98.5%, specificity of 95.4% and kappa value of 0.88 as compared with the serum ELISA. Because high degree of concordance between the serum ELISA and the extract ELISA was observed (P=0.24), extract from lymph nodes could be used as an alternative to serum for the detection of Salmonella antibodies in the ELISA.
Keywords
ELISA; extract of lymph nodes; Salmonella; serum; slaughter pigs;
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1 최원필, 이희석, 여상건, 이헌준, 정석찬. 양돈장에 있어서 Salmonella 감염증의 역학적 연구-1. 발생 및 오염상황, 혈청형과 Salmonella typhimurium의 생물형. 대한수의학회지, 1986, 26, 49-59
2 Bager F, Petersen J. Sensitivity and specificity of different methods for the isolation of Salmonella from pigs. Acta Vet Scand 1991, 32, 473-481
3 Berends B, Van Knapen F, Snijders J, Mossel D. Identification and quantification of risk factors regarding Salmonella spp. on pork carcasses. Int J Food Microbiol 1997, 36, 199-206   DOI   ScienceOn
4 Borch E, Nesbakken T, Christensen H. Hazard identification in swine slaughter with respect to food-borne bacteria. Int J Food Microbiol 1996, 30, 9-25   DOI   ScienceOn
5 Christensen J, Baggesen DL, Soerensen V, Svensmark B. Salmonella level of Danish swine herds based on serological examination of meat-juice samples and Salmonella occurrence measured by bacteriological follow-up. Prev Vet Med 1999, 40, 277-292   DOI   ScienceOn
6 Davies RH, Heath PJ, Coxon SM, Sayers AR. Evaluation of the use of pooled serum, pooled muscle tissue fluid (meat juice) and pooled faeces for monitoring pig herds for Salmonella. J Appl Microbiol 2003, 95, 1016-1025   DOI   ScienceOn
7 Fleiss JL. Statistical Methods for Rates and Proportions. 2nd ed, Wiley. New York, 1981
8 Funk JA, Harris IT, Davies PR. Comparison of fecal culture and Danish Mix-ELISA for determination of Salmonella enterica subsp. enterica prevalence in growing swine. Vet Microbiol 2005, 107, 115-126   DOI   ScienceOn
9 Nielsen B, Baggesen D, Bager F, Haugegaard J, Lind P. The serological response to Salmonella serovars typhimurium and infants in experimentally infected pigs. The time course followed with an indirect anti-LPS ELISA and bacteriological examinations. Vet Microbiol 1995, 47, 205-218   DOI   ScienceOn
10 Nielsen B, Ekeroth L, Bage F, Lind P. Use of muscle fluid as a source of antibodies for serologic detection of Salmonella infection in slaughter pig herds. J Vet Diagn Invest 1998, 10, 158-163   DOI   ScienceOn
11 Sorensen LL, Alban L, Nielsen B, Dahl J. The correlation between Salmonella serology and isolation of Salmonella in Danish pigs at slaughter. Vet Microbiol 2004, 101, 131-141   DOI   ScienceOn
12 Harvey RB, Anderson RC, Farrington LA, Droleskey RE, Genovese KJ, Ziprin RL, Nisbet DJ. Comparison of GN Hajna and tetrathionate as initial enrichment for salmonellae recovery from swine lymph nodes and cecal contents collected at slaughter. J Vet Diagn Invest 2001, 13, 258-260   DOI   ScienceOn
13 Mortensen S, Strandbvgaard B, Botner A, Feld N, Willeberg P. Monitoring porcine reproductive and respiratory syndrome virus infection status in swine herds based on analysis of antibodies in meat juice samples. Vet Res 2001, 32, 441-453   DOI   ScienceOn
14 Sidibe M, Messier S, Lariviere S, Gottschalk M, Mittal KR. Detection of Actinobacillus pleuropneumoniae in the porcine upper respiratory tract as a complement to serological tests. Can J Vet Res 1993, 57, 204-208
15 박선일, 한홍율. 수의 임상역학 및 통계. 한길아카데미. 1999
16 김규태, 정병열, 김봉환. 경북지역 가축에서 분리한 Salmonella 속균의 혈청형 분포 및 약제 감수성. 한국의공중보건학회지. 2003, 27, 47-52
17 Mejia W, Casal J, Mateu E, Martin M. Comparison of two commercial ELISAs for the serological diagnosis of salmonellosis in pigs. Vet Rec 2005, 157, 47-48   DOI
18 Baggesen DL, Wegener HC, Bager F, Stege H, Christensen J. Herd prevalence of Salmonella enterica infections in Danish slaughter pigs determined by microbiological testing. Pre Vet Med 1996, 26, 201-213   DOI   ScienceOn
19 Schwartz KJ. Salmonellosis. In: Straw BE, D'Allaire S, Mengeling WL, Taylor DJ (eds.). Diseases of Swine. 8th ed. pp. 535-551, Iowa State University Press, Ames, 1999
20 Alban L, Stege H, Dahl J. The new classification system for slaughter-pig herds in the Danish Salmonella surveillance-and-control program. Prev Vet Med 2002, 53, 133-146   DOI   ScienceOn
21 Proux K, Houdayer C, Humbert F, Cariolet R, Rose V, Eveno E, Madec F. Development of a complete ELISA using Salmonella lipopolysaccharides of various serogroups allowing to detect all infected pigs. Vet Res 2000, 31, 481-490   DOI   ScienceOn
22 Hoorfar J, Wedderkopp A, Lind P. Detection of antibodies to salmonella lipopolysaccharide in muscle fluid from cattle. Am J Vet Res 1997, 58, 334-337
23 Nilsson LA, Uggla A. Schistosoma mansoni antibodies in tissue specimens demonstrated by a diffusion-in-gel ELISA technique. Vet Parasitol 1992, 45, 73-80   DOI   ScienceOn
24 정재윤, 정병열, 김봉환. Classical swine fever virus pg55 항원에 대한 muscle fluid 항체 측정. 대한수의학회지. 2003, 43, 263-270
25 Thrusfield M, Ortega C, de Blas I, Noordhuizen JP, Frankena K WIN EPISCOPE 2.0: improved epidemiological software for veterinary medicine. Vet Rec 2001, 148, 567-572   DOI   ScienceOn