Browse > Article

Iridovirus infection of cultured juvenile flounder (Paralichthys olivaceus) in nursery  

Kim, Tae-Jung (College of Veterinary Medicine, Chonnam National University)
Jang, Eun-Jin (College of Veterinary Medicine, Chonnam National University)
Kim, Jong-Su (Jeonnam Biotechnology Research Center)
Lee, Jae-Il (College of Veterinary Medicine, Chonnam National University)
Publication Information
Korean Journal of Veterinary Research / v.46, no.1, 2006 , pp. 21-25 More about this Journal
Abstract
Iridovirus is an icosahedral cytoplasmic double-stranded DNA virus with a genome size of 170-200kb. Outbreaks of fish iridovirus infection are characterized by their wide geographic distribution and broad host spectrum, especially in water temperatures of $25-27^{\circ}C$ Recently, the causative agent of high mortalities in flounder (Paralichthys olivaceus) was identified as fish iridovirus in Korea. Iridoviral infection repeatedly occurs in the same area for long periods, suggesting the possibility of viral infection in nursery. To examine this, the existence of iridovirus in juvenile flounders was detected by PCR using virus-specific primers. Antibodies induced against this virus were also examined using ELISA. As a result, juvenile fish in nursery were found to be previously infected with iridovirus, suggesting that proper prevention systems are required.
Keywords
ELISA; iridovirus; PCR; recombinant protein;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Hedrick RP, McDowell TS. Properties of iridoviruses from ornamental fish. Vet Res 1995, 26, 423-427
2 Hyatt AD, Gould AR, Zupanovic Z, Cunningham AA, Hengstberger S, Whittington RJ, Kattenbelt J, Coupar BE. Comparative studies of piscine and amphibian iridoviruses. Arch Virol 2000, 145, 301-331   DOI
3 Nakajima K, Sorimachi M. Biological and physicochemical properties of the iridovirus isolated from cultured red sea bream, Pagrus major. Fish Pathol 1994, 29, 29-33   DOI
4 Oshima S, Hata J, Segawa C, Hirasawa N, Yamashita S. A method for direct DNA amplification of uncharacterized DNA viruses and for development of a viral polymerase chain reaction assay: application to the red sea bream iridovirus. Anal Biochem 1996, 242, 15-19   DOI   ScienceOn
5 Sohn SG, Choi DL, Do JW, Hwang JY, Park JW. Mass mortalities of cultured striped beakperch, Oplegnathus fasciatus by iridoviral infection. J Fish Pathol 2000, 13, 121-127
6 Wang CS, Shih HH, Ku CC, Chen SN. Studies on epizootic iridovirus infection among red sea bream, Pagrus major (Temminck & Schlegel), cultured in Taiwan. J Fish Dis 2003, 26, 127-133   DOI   ScienceOn
7 Williams T. The iridoviruses. Adv Virus Res 1996, 46, 345-412   DOI
8 Studier FW, Moffatt BA. Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes. J Mol Biol 1986, 189, 113-130   DOI
9 Kim YJ, Jung SJ, Choi TJ, Kim HR, Rajendran KV, Oh MJ. PCR amplification and sequence analysis of irido-like virus infecting fish in Korea. J Fish Dis 2002, 25, 121-124   DOI   ScienceOn
10 Nakajima K, Maeno Y, Yokoyama K, Kaji C, Manabe S. Antigen analysis of red sea bream iridovirus and comparison with other fish iridoviruses. Fish Pathol 1998, 33, 73-78   DOI
11 Rodger HD, Kobs M, Macartney A, Frerichs GN. Systemic iridovirus infection in freshwater angelfish, Pterophyllum scalare (Lichtenstein). J Fish Dis 1997, 20, 69-72   DOI   ScienceOn
12 Kim TJ, Lee JI. Cloning and expression of genes encoding transferrin-binding protein A and B from Actinobacillus pleuropneumoniae serotype 5. Protein Expr Purif 2006, 45, 235-240   DOI   ScienceOn
13 Sudthongkong C, Miyata M, Miyazaki T. Viral DNA sequences of genes encoding the ATPase and the major capsid protein of tropical iridovirus isolates which are pathogenic to fishes in Japan, South China Sea and Southeast Asian countries. Arch Virol 2002, 147, 2089-2109   DOI
14 Oshima S, Hata J, Hirasawa N, Ohtaka T, Hirono I, Aoki T, Yamashita S. Rapid diagnosis of red sea bream iridovirus infection using the polymerase chain reaction. Dis Aquat Organ 1998, 32, 87-90   DOI
15 Do JW, Cha SJ, Kim JS, An EJ, Park MS, Kim JW, Kim YC, Park MA, Park JW. Sequence variation in the gene encoding the major capsid protein of Korean fish iridoviruses. Arch Virol 2005, 150, 351-359   DOI
16 He JG, Deng M, Weng SP, Li Z, Zhou SY, Long QX, Wang XZ, Chan SM. Complete genome analysis of the mandarin fish infectious spleen and kidney necrosis iridovirus. Virology 2001, 291, 126-139   DOI   ScienceOn
17 Oh MJ, Jung SJ, Kim YJ. Detection of RSIV (Red Sea Bream Iridovirus) in the cultured marine fish by the polymerase chain reaction. J Fish Pathol 1999, 12, 66-69
18 Seferian PG, Martinez ML. Immune stimulating activity of two new chitosan containing adjuvant formulations. Vaccine 2000, 19, 661-668   DOI   ScienceOn
19 김태중, 이봉주, 이재일. 대장균에서 흉막폐렴균 독소 Apx I과 Apx II의 대량발현. 대한수의학회지 2005, 45, 185-189
20 Nakajima K, Inouye K, Sorimachi M. Viral diseases in cultured marine fish in Japan. Fish Pathol 1998, 33, 181-188   DOI
21 Sambrook J, Fritsch EF, Maniatis T. Molecular cloning: a laboratory manual. 2nd ed, Cold Spring Harbor Laboratory Press, New York, 1989
22 Do JW, Moon CH, Kim HJ, Ko MS, Kim SB, Son JH, Kim JS, An EJ, Kim MK, Lee SK, Han MS, Cha SJ, Park MS, Park MA, Kim YC, Kim JW, Park JW. Complete genomic DNA sequence of rock bream iridovirus. Virology 2004, 325, 351-363   DOI   ScienceOn
23 Jung SJ, Oh HJ. Iridovirus-like infection associated with high mortalities of striped beakperch, Oplegnathus fasciatus (Temminck et Schlegel), in southern coastal areas of the Korean peninsula. J Fish Dis 2000, 23, 223-226   DOI   ScienceOn
24 Matsuoka S, Inouye K, Nakajima K. Cultured fish species affected by red sea bream iridoviral disease from 1991 to 1995. Fish Pathol 1996, 31, 233-234   DOI
25 Miyata M, Matsuno K, Jung SJ, Danayadol Y, Miyazaki T. Genetic similarity of iridoviruses from Japan and Thailand. J Fish Dis 1997, 20, 127-134   DOI   ScienceOn