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Expression of caveolin-3 as positive intracellular signaling regulator on the development of hypertrophy in cardiac tissues  

Kim, Joo-Heon (Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University)
Han, Jin (Cardiovascular & Metabolic Disease Center, College of Medicine, Inje University)
Kim, Yong-Kwon (College of Biomedical Science and Engineering, Inje University)
Yang, Young-Ae (College of Biomedical Science and Engineering, Inje University)
Hong, Yonggeun (Cardiovascular & Metabolic Disease Center, College of Medicine, Inje University)
Publication Information
Korean Journal of Veterinary Research / v.45, no.4, 2005 , pp. 537-544 More about this Journal
Abstract
We have examined distribution and expression of caveolin-3 (cav-3), one of three caveolin isoforms from 16-wks-old spontaneously hypertensive rats (SHR) compared with age-matched control wistar-kyoto (WKY) rats. The expression of cav-3 was increased, whereas expression of PKB/Akt and calcineurin (Cn) was not changed in cardiac tissues of SHR compared to WKY rats. Interestingly, expression of cav-3, PKB/Akt and Cn were decreased in plasma membrane fraction in SHR compared to WKY rats. In H9c2 cardiomyoblast cells treated with phenylephrine ($50{\mu}M$, 48hr) or isoproterenol ($10{\mu}M$, 48hr), the expression of cav-3 was markedly enhanced compared to nontreated cells. Upon immunofluorescence analysis, cav-3 was localized in plasma membrane of control H9c2 cells. However phenylephrine or isoproterenol treatment caused translocation of cav-3 to perinuclear region. These results suggest that cav-3 plays as positive regulators in the development of hypertrophy in cardiac tissues of SHR rats.
Keywords
calcineurin; caveolin; hypertrophy; SHR; spontaneously hypertensive rat; PKB/Akt;
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