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Phospholipase C-mediated vasorelaxing action of melatonin in rat isolated aorta  

Kim, Shang-Jin (Bio-Safety Research Institute, Chonbuk National University)
Baek, Sung-Soo (Bio-Safety Research Institute, Chonbuk National University)
Kang, Hyung-Sub (Bio-Safety Research Institute, Chonbuk National University)
Kim, Jin-Shang (Bio-Safety Research Institute, Chonbuk National University)
Publication Information
Korean Journal of Veterinary Research / v.45, no.4, 2005 , pp. 507-515 More about this Journal
Abstract
Melatonin, the principal hormone of the vertebral pineal gland, participates in the regulation of cardiovascular system in vitro and in vivo. However, the effects of melatonin on vascular tissues are still vague. The aim of this study was to assess the relationship between phospholipase C (PLC) and nitric oxide synthase (NOS)/cyclic guanosine 3',5'-monophosphate (cGMP) signaling cascade in the relaxatory action of melatonin in isolated rat aorta. Melatonin induced a concentration-dependent relaxation in phenylephrine (PE)- and KCl-precontracted endothelium intact (+E) aortic rings. In KCl-precontracted +E aortic rings, the melatonin-induced vasorelaxation was not inhibited by endothelium removal or by pretreatment with NOS inhibitors, L-$N^G$-nitor-arginine (L-NNA) and L-$N^G$-nitor-arginine methyl ester (L-NAME), guanylate cyclase (GC) inhibitors, methylene blue (MB) and 1H-[1,2,4] oxadiazolo-[4,3-a] quinoxalin-1-one (ODQ). In PE-precontracted +E aortic rings, the melatonin-induced vasorelaxation was inhibited by endothelium removal or by pretreatment with L-NNA, L-NAME, MB, ODQ and 2-nitro-4-carboxyphenyl-n,n-diphenylcarbamate (NCDC). Moreover, in without endothelium (-E) aortic rings and in the presence of L-NNA, L-NAME, MB and ODQ in +E aortic rings, the melatonin-induced residual relaxations and residual contractile responses to PE were not affected by NCDC, a PLC inhibitor. It is concluded that melatonin can evoke vasorelaxation due to inhibition of PLC pathway through the protein kinase G activation of endothelial NOS/cGMP signaling cascade.
Keywords
aorta; cGMP; melatonin; NOS; 2-nitro-4-carboxyphenyl-n,n-diphenylcarbamate; vasorelaxation;
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1 Bosman H, Dormehl IC, Hugo N, Redelinghuys IF, Theron JJ. The effect of intravenous administration of melatonin on cardiovascular parameters of the baboon (Papio ursinus). J Pineal Res 1991, 11, 179-181   DOI   PUBMED
2 Godson C, Reppert SM. The Mella melatonin receptor is coupled to parallel signal transduction pathways. Endocrinology 1997, 138, 397-404   DOI   ScienceOn
3 Martinuzzo M, Del Zar MM, Cardinali DP, Carreras LO, Vacas MI. Melatonin effect on arachidonic acid metabolism to cyclooxygenase derivatives in human platelets. J Pineal Res 1991, 11, 111-115   DOI   PUBMED
4 McLellan TM, Gannon GA, Zamecnik J, Gil V, Brown GM. Low doses of melatonin and diurnal effects on thermoregulation and tolerance to uncompensable heat stress. J Appl Physiol 1999, 87, 308-316
5 Nakajima T, Hazama H, Hamada E, Wu SN, Igarashi K, Yamashita T, Seyama Y, Omata M, Kurachi Y. Endothelin-1 and vasopressin activate $Ca^{2+}$-permeable non-selective cation channels in aortic smooth muscle cells: mechanism of receptor-mediated $Ca^{2+}$ influx. J Mol Cell Cardiol 1996, 28, 707-722   DOI   ScienceOn
6 Pozo D, Reiter RJ, Calvo JR, Guerrero JM. Inhibition of cerebellar nitric oxide synthase and cyclic GMP production by melatonin via complex formation with calmodulin. J Cell Biochem 1997, 65, 430-442   DOI   ScienceOn
7 Reiter RJ. Pineal melatonin: cell biology of its synthesis and of its physiological interactions. Endocr Rev 1991, 12, 151-180   DOI   PUBMED   ScienceOn
8 Reiter RJ, Calvo JR, Karbownik M, Oi W, Tan DX. Melatonin and its relation to the immune system and inflammation. Ann N Y Acad Sci 2000, 917, 376-386   DOI
9 Satake N, Shibata S, Takagi T. The inhibitory action of melatonin on the contractile response to 5-hydroxytryptamine in various isolated vascular smooth muscles. Gen Pharmacol 1986, 17, 553-558   DOI   ScienceOn
10 Somlyo AP, Himpens B. Cell calcium and its regulation in smooth muscle. FASEB J 1989, 3, 2266-2276
11 Vanecek J. Cellular mechanisms of melatonin action. Physiol Rev 1998, 78, 687-720
12 Underwood H, Goldman BD. Vetebrate circadian and photoperiodic system: role of the pineal gland and melatonin. J Biol Rhythms 1987, 2, 279-315   DOI   ScienceOn
13 Girouard H, Chulak C, Lejossec M, Lamontagne D Lamontagne D, De Champlain J. Vasorelaxant effects of the chronic treatment with melatonin on mesenteric artery and aorta of spontaneously hypertensive rats. J Hypertens 2001, 19, 1369-1377   DOI   ScienceOn
14 Faillace MP, Keller Sarmiento MI, Rosenstein RE. Melatonin effect on the cyclic GMP system in the golden hamster retina. Brain Res 1996, 711, 112-117   DOI   PUBMED   ScienceOn
15 Holmes SW, Sugden D. The effect of melatonin on pinealectomy-induced hypertension in the rat. Br J Pharmacol 1976, 56, 360-361
16 Cauvin C, Loutzenhiser R, Van Breemen C. Mechanism of calcium-antagonist induced vasodilation. Annu Rev Pharmacol 1983, 23, 373-396   DOI   ScienceOn
17 Weekley B. Pharmacologic studies on the mechanism of melatonin-induced vasorelaxation in rat aorta. J Pineal Res 1995, 19, 133-138   DOI   PUBMED
18 Bitman EL, Karsch EJ. Nightly duration of pineal melatonin secretion determines the reproductive response to inhibitory day length in the ewe. Biol Repord 1984, 30, 585-593   DOI   ScienceOn
19 Doolen S, Krause DN, Dubocovich ML, Duckles SP. Melatonin mediates two distinct responses in vascular smooth muscle. Eur J Pharmacol 1998, 345, 67-69   DOI   ScienceOn
20 Okatani Y, Wakatsuki A, Watanabe K, Taniguchi K, Fkaya T. Weak vasoconstrictor activity of melatonin in human umbilical artery: relation to nitric oxidescavenging action. Eur J Pharmacol 2001, 417, 125-129   DOI   PUBMED   ScienceOn
21 Kawashima K, Miwa Y, Fujimoto K, Oohata H, Nishino H, Koike H. Antihypertensive action of melatonin in the spontaneously hypertensive rat. Clin Exp Hypertens 1987, 9, 1121-1131   DOI
22 Carvajal JA, Germain AM, Huidobro-Toro JP, Weiner CP. Molecular mechanism of cGMP-mediated smooth muscle relaxation. J Cell Physiol 2000, 184, 409-420   DOI   ScienceOn
23 Chuang JI, Chen SS, Lin MT. Melatonin decreases brain serotonin release, arterial pressure and heart rate in rats. Pharmacology 1993, 47, 91-97   DOI   ScienceOn
24 Cunnane SC, Manku MS, Oka M, Horrobin DP. Enhanced vascular reactivity to various vasoconstrictor agents following pinealectomy in the rat: role of melatonin. Can J Physiol Pharmacol 1980, 58, 287-293   DOI   ScienceOn
25 Satake N, Oe H, Shibata S. Vasorelaxing action of melatonin in rat isolated aorta; possible endothelium dependent relaxation. Gen Pharmacol 1991, 22, 1127-1133   DOI   ScienceOn
26 Geary GG, Krause DN, Duckles SP. Melatonin directly constricts rat cerebral arteries through modulation of potassium channels. Am J Physiol 1997, 273, 1530-1536
27 Dart AM, Kingwell BA. Pulse pressure-a review of mechanisms and clinical relevance. J Am Coll Caridiol 2001, 37, 975-984   DOI   ScienceOn
28 Reiter RJ, Tan DX. Melatonin: a novel protective agent against oxidative injury of the ischemic/reperfused heart. Cardiovasc Res 2003, 58, 10-19   DOI   ScienceOn
29 Pozo D, Reiter RJ, Calvo JR, Guerrero JM. Physiological concentrations of melatonin inhibit nitric oxide synthase in rat cerebellum. Life Sci 1994, 55, 455-460   DOI   ScienceOn
30 Weekley B. Effects of melatonin on isolated pulmonary artery and vein; role of vascular endothelium. Pulm Pharmacol 1993, 6, 149-154   DOI   PUBMED   ScienceOn
31 Satake N, Sawada HOT, Shibata S. The mode of vasorelaxing action of melatonin in rabbit aorta. Gen Pharmacol 1991, 22, 219-221   DOI   ScienceOn
32 Karaki H, Ozaki H, Hori M, Mitsui-Saito M, Amano K, Harada K, Miyamoto S, Nakazawa H, Won KJ, Sato K. Calcium movements, distribution, and functions in smooth muscle. Pharmacol Rev 1997, 49, 157-230
33 Benitez-King G, Rios A, Martinez A, Anton-Tay F. In vitro inhibition of $Ca^{2+}$/calmodulin-dependent kinase II activity by melatonin. Biochim Biophys Acta 1996, 1290, 191-196   DOI   ScienceOn
34 Lusardi P, Piazza E, Fogari R. Cardiovascular effects of melatonin in hypertensive patients well controlled by nifedipine: a 24-hour study. Br J Clin Pharmacol 2000, 49, 423-427   DOI   ScienceOn
35 Kilic E, Ozdemir YG, Bolay H, Kelestimur H, Dalkara T. Pinealectomy aggravates and melatonin administration attenuates brain damage in focal ischemia. J Cereb Blood Flow Metab 1999, 19, 511-516   DOI