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Coexpression of PCNA and p21 for DNA repair in small intestinal crypt cells of mouse with 60Co γ-rays irradiation  

Hong, Suji (Department of Veterinary Medcine, College of Applied Life Sciences and Applied Radiological Science Research Institute, Cheju National University)
Hwang, Insun (Department of Veterinary Medcine, College of Applied Life Sciences and Applied Radiological Science Research Institute, Cheju National University)
Ahn, Meejung (Department of Veterinary Medcine, College of Applied Life Sciences and Applied Radiological Science Research Institute, Cheju National University)
Shin, Taekyun (Department of Veterinary Medcine, College of Applied Life Sciences and Applied Radiological Science Research Institute, Cheju National University)
Joo, Hong-gu (Department of Veterinary Medcine, College of Applied Life Sciences and Applied Radiological Science Research Institute, Cheju National University)
Park, HyunJeong (Department of Veterinary Medcine, College of Applied Life Sciences and Applied Radiological Science Research Institute, Cheju National University)
Jee, Youngheun (Department of Veterinary Medcine, College of Applied Life Sciences and Applied Radiological Science Research Institute, Cheju National University)
Publication Information
Korean Journal of Veterinary Research / v.45, no.4, 2005 , pp. 457-464 More about this Journal
Abstract
The irradiation of radioactive ${\gamma}-ray$ induces apoptosis of radiosensitive organs for homeostasis. In this study, we investigated the repair mechanisms for homeostasis in the small intestine after cell damage by $^{60}Co\;{\gamma}-ray$ irradiation. The apoptosis was most frequently observed in the crypt cells of the small intestine after four and six hours by radioactive ${\gamma}-ray$ irradiation, and the frequency of apoptosis was proportional to the amount of irradiation. Also, the number of apoptotic cells was coincident with expression pattern of p53. Interestingly, PCNA (proliferating cell nuclear antigen) which is engaged in DNA replication and repair was expressed in apoptotic cells of small intestinal crypts. Also, it was observed that cell-cycle regulator p21 which is known to induce cell-cycle arrest is co-expressed in the same apoptotic cells of irradiated small intestinal crypt cells. These findings suggest that the co-expression of PCNA and p21 proteins, which may lead to resistance to DNA damage through cell-cycle arrest is closely associated with repair of damaged gastrointestinal cells after ${\gamma}-ray$ irradiation.
Keywords
apoptosis; $^{60}Co\{\gamma}-ray$; small intestine; crypt cells; PCNA (proliferating cell nuclear antigen); p21;
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