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Antioxidative effects of traditional medicinal plants on lipid peroxidation  

Hah, Dae-sik (Gyeongnam Provincial Government Institute of Health and Environment)
Kim, Chung-hui (Department of Animal Science and Biotechnology, Jinju National University)
Kim, Gon-sup (Collage of Veterinary Medicine and Institute of Animal Science, Gyeongsang National University)
Kim, Eui-gyung (Collage of Veterinary Medicine and Institute of Animal Science, Gyeongsang National University)
Kim, Jong-shu (Collage of Veterinary Medicine and Institute of Animal Science, Gyeongsang National University)
Publication Information
Korean Journal of Veterinary Research / v.45, no.3, 2005 , pp. 341-350 More about this Journal
Abstract
To assess the antioxidative activity of 12 medicinal plants on lipid peroxidation, twelves traditional medicinal plants extracted with 95% methanol were investigated the antioxidative activity using DPPH, thiocyanate acid method, and thiobarbituric acid (TBA) methods. Out of 12 medicinal plants extracted with methanol, the extraction yields of Sedum kamtschaticum was the highest values (49.46%) among them and Geranicum sibiricum, Saururus chinensis root (R), Agrimonia pilosa leaf (L), Agrimonia pilosa root was the lowest value (9.97%). Radical scavenging effect of the selected traditional medicinal plants extracted from different extract solution were examined by 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical method. Antioxidative activity of methanolic extracts was higher than those of ethanol and n-hexane extracts. Scavenging effects in Sedum kamtaschaticum (R) determined by DPPH radical showed the highest among the 12 plants. The antioxidative effects of the first four medicinal plants were similar to those of butylated hydroxyanisole (BHA), and butylated hydroxytoluene (BHT), but higher than that of tocopherol, which was used as a handled control. Antioxidative effects of each indicated concentration of the methanolic extracts on linoleic acid by thiocyanate method was the highest in Sedum kamtschaticum and followed by Geum japonicum and Agrimonia pilosa and their antioxidative effect were similar to those of BHA, and BHT, but higher than that of tocopherol. Antioxidative effects of the selected medicinal methanolic extract on linoleic acid by thiocyanate acid method were examined for 15 days. Peroxidation of control and tocopherol group occurred on days 5 and 9, respectively, but BHA, BHT, selected medicinal methanolic extract group did not occur until on day 15. Antioxidative effects of the selected medicinal methanolic extract on linoleic acid by TBA method were examined for 15 days. Antioxidative activity was similar to those obtained by thiocyanate acid method.
Keywords
Antioxidative; traditional medicinal plants; TBA; thiocyanate;
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1 강우석, 김정한, 박은주, 윤광로. 울금 에탄놀 추출물의 항산화 활성 비교. 한국식품과학회지. 1998, 30, 266-271
2 육홍선, 김성애, 조성기, 변맹우. 감마선 조사된 홍삼 분말의 항산화 효 과 및 유전독성학적 안전성. 한국식품위생학회지. 1996, 11, 41-50
3 허문영. 인삼지용성 성분의 지질과산화 및 산화적 DNA 손상에 대한 억제효과. 한국식품위생학회지. 1997, 12, 315-320
4 Floyd RA. Role of oxygen free radicals in carcinogenesis and brain ischemia. FASEB J 1990, 4, 2587-2597
5 Fujimoto K, Kaneda T. Screening test for antioxygenic compounds from marine algae and fractionation from Eisenia bicyclis and Undaria Pinnatifida. Bull Jpn Soc Sci Fish 1980, 46, 1125-1135   DOI
6 Pratt DE, Birac PM. Source of antioxidant activity of soybeans and soy products. J Food Sci 1979, 44. 1720-1725   DOI
7 Nobuyki S, Shunji U, Yoshinori F, Masayasu S. Protective effect of vitamin E on chromium (VI)-induced cytotoxicity and lipid peroxidation in primary cultures of rat hepatocytes. Arch Toxicol 1996, 71, 20-24   DOI   ScienceOn
8 Mitsuda H, Yasumoto K, Iwami K. Antioxidative action of indole compounds during the autoxidation of linoleic acid. Eiyo Shok 1966, 19, 210-215   DOI
9 최용화, 김명조, 이행순, 胡昌序, 곽상수. 해당화 (Rosarugosa) 잎의 항산화물질. 생약학회지. 1997, 28, 179-184
10 Glass GA, Stark AA. Promotion of glutathion-$\Upsilon$-gluatmyl transpeptidase-dependent lipid peroxidation by copper and ceruloplasmin : The requirement for iron and the effects of antioxidants and antioxidant enzymes. Environ Mol Mutagen. 1997, 29, 73-80   DOI   ScienceOn
11 Wu G, Morris SM. Arginine metabolism : Nitric oxide and beyond. Biochem J 1998, 336, 1-6   DOI
12 Ignarro LJ, Cirino G, Casini A. Nitric oxide as a signaling molecule in the vascular system. J Cardiovasc Pharmacol 1999, 34, 879-889   DOI   ScienceOn
13 Lander HM. An essential role for free radicals and derived species in signal transduction. FASEB J 1997, 11, 118-126
14 Yun ZF, Sheng Y, Guoyao W. Free radicals, antioxidants, and nutrition. Nutrition 2002, 18, 872-879   DOI   ScienceOn
15 차배천, 이성규 이혜원, 이은, 최무영, 임태진, 박희준. 국내 유용식물의 항산화 효과. 생약학회지. 1998, 28, 15-20
16 Masaki H, Sakaki S, Atsumi T, Sakurai H. Activeoxygen scavenging activity of plant extracts. Biol Pharm Bull 1997, 18, 162-166
17 Branen AL. Toxicology and biochemistry of butylated hydroxy Anisole and butylated hydroxytoluene. J Am Oil Chem Soc. 1975, 52. 59-63   DOI   PUBMED
18 Kumpawat K, Deb S, Ray S, Chatterjee A. Genotoxic effect of raw betel-nut extract in relation to endogenous glutathione levels and its mechanism of action in mammalian cells. Mut at Res 2003, 538, 1-12   DOI   ScienceOn
19 Selvendiran KJ, Singh PV, Kirshnan KB, Sakthisekaran D. Cytoprotective effect of piperine against beno[a]pyrene induced lung cancer with reference to lipid peroxidation and antioxidant system in Swiss albino mice. Fitoter 2003, 74, 109-115   DOI   ScienceOn
20 Wu G, Flynn AW, Jolly CA. Dietary protein or arginine deficiency impairs constitutive and inducible nitric oxide synthesis by young rats. J Nutr 1999, 1239, 1347-1357
21 Neuzil J, Gebicki JM, Stocker R. Radical-induced chain oxidation of proteins and its inhibition by chain breaking antioxidants. J Biochem 1993, 293, 601-606   DOI
22 맹영선, 박혜경. 더덕 에탄올추출물의 항산화효과. 한국식품과학회지 1991, 23, 311-316
23 Pratt DE, Watts BW. The antioxidative activity of vegetable extracts. J Food Sci 1975, 29, 17-24
24 Freidovich I. Fundamental aspects of reactive oxygen species, or what's the matter with oxygen? Ann NAY Acad Sci 1999, 893, 13-15   DOI   PUBMED
25 Kimie I, Tachio A, Masaki S, Yoshisa K, Ryokei K, Toshiro M. Antioxidative effect of protoporphyrin on lipid rat liver subcellular fractions. Chem Pharm Bull 1988, 36, 1104-1109   DOI   ScienceOn
26 Fang YZ. Effect of ionizing radiation on superoxide dismutase in vitro and in vivio. Adv Free Radic Biol Med 1991, 1, 1-6   DOI   ScienceOn
27 Inatani R, Nakatani N, Fuwa H. Antioxidative effect the constituent of rosemary and their derivatives. Agric Biol Chem 1996, 47, 521-528
28 Ohta N, Kuwata G, Akahori H, Watanabe T. Isolation of new isoflavane acetyl glucoside 6'-O-acetyl genistin from soybeans. Agri Biol Chem 1980, 44, 469-470   DOI
29 Steinmetz KA, Potter JD. Vegetables, fruit and cancer. II. mechanisms. Cancer Causes Control 1991, 2, 427-442   DOI   ScienceOn
30 Wen HC, Beth AV, Xin GL. High level dietary vitamin E do not replace cellular glutathione peroxidase in protecting mice from acute oxidative stress. Biochem Mole Action Nut 1999, 98, 1951-1957
31 Fukuzawa K, Takaishi Y. Antioxidants. J Act Oxyg Free Rad. 1990, 1, 55-70
32 Daniel G, Isabella DD, Roberto C, Aldo M, Raniel R. An improved HPLC measurement for GSH and GSSG in human blood. Free Radic Biol Med 2003, 35, 1365-1372   DOI   ScienceOn
33 Hatano T. Constituents of natural medicines with Scavenging effects on active oxygen species-tannins and related polyphenols. Nutr Med. 1995, 49. 357-363
34 윤석권, 김정한, 김재욱. 탈지들깨박 Ethanol 추출물의 항산화 효과. 한국식품과학회지. 1993, 25, 160-164
35 MeCord JM. The evolution of free radicals and oxidative stress. Am J Med 2000, 108, 652-662   DOI   PUBMED   ScienceOn
36 박재한, 강규찬, 백상봉, 이윤형, 이규순. 식용해조류에서 항산화 물질의 분리. 한국식품과학회지. 1991, 23, 256-261
37 Zheng M, Storz, G. Redox sensing by prokaryotic transcription factors. Biochem Pharmacol 2000, 59, 1-8   DOI   PUBMED   ScienceOn
38 차배천, 박희준, 이은, 최무영, 임태진. 대두와 돌콩의 항산화 활성 및 성분 비교. 생약회지. 1996, 27, 190-195