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Development of the immunochromatographic strip for the rapid detection of Listeria spp.  

Jung, Byeong-yeal (Veterinary Pharmacology Division, National Veterinary Research and Quarantine Service, Ministry of Agriculture and Forestry)
Jung, Suk-chan (Livestock Products Standard Division, National Veterinary Research and Quarantine Service, Ministry of Agriculture and Forestry)
Kim, Jong-man (Bacteriology and Parasitology Division, National Veterinary Research and Quarantine Service, Ministry of Agriculture and Forestry)
Publication Information
Korean Journal of Veterinary Research / v.45, no.2, 2005 , pp. 169-177 More about this Journal
Abstract
We developed the one-step strip based on an immunochromatographic (IC) assay for the rapid detection of Listeria spp. Genus-specific monoclonal antibody to flagella of L. monocytogenes was conjugated with 40 nm colloidal gold particles which were prepared in our laboratory. The specificity of the IC strip was tested with pure cultured bacteria. All strains of the genus of Listeria spp. yielded positive reactions and 12 strains of non-Listeria were negative, resulting in a specificity of 100%. L. monocytogenes was artificially inoculated in raw pork macerated with listeria enrichment broth. And then it was 10-fold diluted from $8.7{\times}10^6$ to 8.7 CFU/ml. L. monocytogenes could be detected at a minimum of $8.7{\times}10^5CFU/ml$ before enrichment, $8.7{\times}10^2CFU/ml$ after primary enrichment and 8.7 CFU/ml after secondary enrichment, respectively. These results indicated that the IC strip exhibited high specificity and sensitivity in the detection of Listeria spp.
Keywords
Listeria; immunochromatographic strip; colloidal gold;
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