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Detection of viral pathogens and isolation of porcine circovirus 2 from postweaning multisystemic wasting syndrome-affected piglets  

Park, Choi-Kyu (National Veterinary Research and Quarantine Services)
Kim, Hyun-Soo (College of Veterinary Medicine, Chungnam National University)
Publication Information
Korean Journal of Veterinary Research / v.44, no.4, 2004 , pp. 561-569 More about this Journal
Abstract
To detect viral agents and isolate porcine circovirus 2 (PCV2), 60 samples of lung and lymph node were collected from 5 to 12 week-old pigs that had showed clinical signs of postweaning multisystemic wasting syndrome (PMWS). Polymerase chain reactions (PCRs) were conducted to identify the viral pathogens including PCV1, PCV2, porcine parvovirus (PPV) and porcine reproductive and respiratory syndrome virus (PRRSV) that have been considered to be the causal agents of PMWS. Among 60 samples, PCV 2 was detected from 57 samples but no PCV 1 was detected. PRRSV and/or PPV were also detected from 27 (47.4%) samples and 1 (1.8%) sample of these 57 PCV 2-positive samples, respectively. Tissue homogenates were inoculated onto PCV-free PK-15 cell monolayers. Seven isolates were confirmed as PCV 2 by multiplex PCR, indirect immunofluorescence assay, and transmissible electron microscopy. These date suggest that PRRSV is a major cofactors causing PMWS in pigs that were infected with PCV2 in Korea.
Keywords
postweaning multisystemic wasting syndrome(PMWS); porcine circovirus(PCV); porcine parvovirus( PPV); porcine reproductive and respiratory syndrome virus(PRRSV); polymerase chain reaction(PCR);
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1 Allan, G. M. and Ellis, J. A. Porcine circoviruses: a review. J. Vet. Diagn. Invest. 2000, 12, 3-14
2 Ellis, J., Hassard, L., Clark, W., Harding, J., Allan, G., Willson, P., Strokappe, J., Martin, K., McNeilly, F., Meehan, B., Todd, D. and Haines, D. Isolation of circovirus from lesions of pigs with postweaning multisystemic wasting syndrome. Can. Vet. J. 1998, 39, 41-51
3 Krakowka, S., Ellis, J. A., Meehan, B., Kennedy, S., McNeilly, F. and Allan, G. Viral wasting syndrome of swine: experimental reproduction of postweaning multisystemic wasting syndrome in gnotobiotic swine by coinfection with porcine circovirus 2 and porcine parvovirus. Vet. Pathol. 2000, 37, 254-263
4 LeCann, P., Albina, E., Madec, F., Cariolet, R. and Jestin, A. Piglet wasting disease. Vet. Rec. 1997, 141, 660
5 Lukert, P., de Boer, G. F., Dale, J. L.. The Circoviridae. In: Murphy, F. A., Fauquet, C. M. and Bishop, D. H. L. Virus Toxonomy. 6th Rep. Int. Committee Taxon. viruses. pp. 166-168, Vienna: Springer-Verlag, 1995
6 Mankertz, A., Domingo, M., Folch, J. M., LeCann, P., Jestin, A., Segales, J., Chmielewicz, B., Plana-Duran, J. and Soike, D. Characterisation of PCV-2 isolates from Spain, Germany and France. Virus Res. 2000, 66, 65-77
7 Sambrook, J. F., Russell, D. W. and Irwin, N. Molecular Cloning: A laboratory Manual. 3rd ed, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, 2000
8 Sorden, S. D., Harms, P. A., Nawagitgul, P., Cavanaugh, D. and Paul, P. S. Development of a polyclonal-antibody-based immunohistochemical method for the detection of type 2 porcine circovirus in formalin-fixed, paraffin-embeded tissue. J. Vet. Diagn. Invest. 1999, 11. 528-530
9 Tischer, I., Bode, L., Peters, D., Pociuli, S. and Germann B. Distribution of antibodies to porcine circovirus in swine populations of different breeding farms. Arch. Virol. 1995, 140, 737-743
10 Tischer, I., Rasch, R. and Tochtermann, G. Characterization of papovavirus and picornavirus-like particles in permanent piglet kidney cell lines. Zentralbl. Bakteriol. Orig. A. 1974, 226, 153-167
11 Allan, G. M., Kennedy, S., McNeilly, F., Foster, J. C., Ellis, J. A., Krakowka, S. J., Meehan, B. M. and Adair, B. M. Experimental reproduction of severe wasting disease by co-infection of pigs with porcine circovirus and porcine parvovirus. J. Comp. Pathol. 1999, 121, 1-11
12 McNeilly, F., McNair, I., Mackie, D. P., Meehan, B. M., Kennedy, S., Moffett, D., Ellis, J., Krakowka, S. and Allan, G. M. Production, characterisation and applications of monoclonal antibodies to porcine circovirus 2. Arch. Virol. 2001, 146, 909-922
13 Nayar, G. P., Hamel, A. and Lin, L. Detection and characterization of porcine circovirus associated with postweaning multisystemic wasting syndrome in pigs. Can. Vet. J. 1997, 38, 385-386
14 Balasch, M., Segales, J., Rosell, C., Domingo, M., Mankertz, A., Urniza, A. and Plana-Duran, J. Experimental inoculation of conventional pigs with tissue homogenates from pigs with post-weaning multisystemic wasting syndrome. J. Comp. Pathol. 1999, 121, 139-148
15 Choi, C. and Chae, C. In-situ hybridization for the detection of porcine circovirus in pigs with postweaning multisystemic wasting syndrome. J. Comp. Pathol. 1999, 21, 65-270
16 Hamel, A. L., Lin, L. L. and Nayar, G. P. Nucleotide sequence of porcine circovirus associated with postweaning multisystemic wasting syndrome in pigs. J. Virol. 1998, 72, 5262-5267
17 Tischer, I., Bode, L., Apodaca, J., Timm, H., Peters, D., Rasch, R., Pociuli, S. and Gerike, E. Presence of antibodies reacting with porcine circovirus in sera of humans, mice, and cattle. Arch. Virol. 1995, 140, 1427-1439
18 Harding, J. Post-weaning multisystemic wasting syndrome (PMWS): preliminary epidemiology and clinical presentation. Proc. Am. Assoc. Swine Pract. 1997, 28, 503
19 Hines, R. K. and Lukert, D. Porcine circovirus: A serological survey of swine in the United States. Swine Health Prod. 1995, 3, 71-73
20 Lukert, P. D. Disease of swine. pp. 119-131, 8th ed. Iowa State University Press, Ames, Iowa, 1999
21 Onuki, A., Abe, K., Togashi, K., Kawashima, K., Taneichi, A. and Tsunemitsu, H. Detection of porcine circovirus from lesions of a pig with wasting disease in Japan. J. Vet. Med. Sci. 1999, 61, 1119-1123
22 Morozov, I., Sirinarumitr, T., Sorden, S. D., Halbur, P. G., Morgan, M. K., Yoon, K. J. and Paul, P. S. Detection of a novel strain of porcine circovirus in pigs with postweaning multisystemic wasting syndrome. J. Clin. Microbiol. 1998, 36, 2535-2541
23 Ellis, J. A., Bratanich, A., Clark, E. G., Allan, G., Meehan, B., Haines, D. M., Harding, J., West, K. H., Krakowka, S., Konoby, C., Hassard, L., Martin, K. and McNeilly, F. Coinfection by porcine circoviruses and porcine parvovirus in pigs with naturally acquired postweaning multisystemic wasting syndrome. J. Vet. Diagn. Invest. 2000, 12, 21-27
24 Choi, C., Chae, C. and Clark, E. G. Porcine postweaning multi-systemic wasting syndrome in Korean pig: detection of porcine circovirus 2 infection by immunohistochemistry and polymerase chain reaction. J. Vet. Diagn. Invest. 2000, 12, 151-153
25 Dulac, G. C. and Afshar, A. Porcine circovirus antigens in PK-15 cell line (ATCC CCL-33) and evidence of antibodies to circovirus in Canadian pigs. Can. J. Vet. Res. 1989, 53, 431-433
26 Park, C. K., Joo, H. D., Shin, J. H., Kim, J. H., Sohn, H. J., Kim, K. S. and Kang, Y. B. Annual research report of National Veterinary Research and Qurantine Service (NVRQS) in 1999. NVRQS, Korea. 2000
27 Lyoo, Y. S., Park, C. K. and Lee, C. H. RT-PCR and nested PCR amplification of the PRRSV genes from boar semen for the rapid and sensitive differential diagnosis. Korean J. Vet. Res. 1998, 38, 77-83
28 Stevenson, G. W., Kiupel, M., Mittal, S. K. and Kanitz, C. L. Ultrastructure of porcine circovirus in persistently infected PK-15 cells. Vet. Pathol. 1999, 36, 368-378
29 Allan, G. M., McNeilly, F., Kennedy, S., Daft, B., Clark, E. G., Ellis, J. A., Haines, D. M., Meehan, B. M. and Adair B. M. Isolation of porcine circoviruslike viruses from pigs with a wasting disease in the USA and Europe. J. Vet. Diagn. Invest. 1998, 10, 3-10
30 Walker, I. W., Konoby, C. A., Jewhurst, V. A., McNair, I., McNeilly, F., Meehan, B. M., Cottrell, T. S., Ellis, J. A. and Allan, G. M. Development and application of a competitive enzyme-linked immunosorbent assay for the detection of serum antibodies to porcine circovirus type 2. J. Vet. Diagn. Invest. 2000, 12, 400-405
31 Ouardani, M., Wilson, L., Jette, R., Montpetit, C. and Dea, S. Multiplex PCR for detection and typing of porcine circoviruses. J. Clin. Microbiol. 1999, 37, 3917-3924
32 Madec, F., Eveno, E., Morvan, P., Hamon, L., Blanchard, P., Cariolet, R., Amenna, N., Morvan, H., Truong, C., Mahe, D., Albina, E. and Jestin, A. Post-weaning multisystemic wasting syndrome (PMWS) in pigs in France: clinical observations from follow-up studies on affected farms. Livest. Prod. Sci. 2000, 63, 223-233
33 Tischer, I., Mields, W., Wolff, D., Vagt, M. and Griem, W. Studies on epidemiology and pathogenicity of porcine circovirus. Arch. Virol. 1986, 91, 271-276
34 Tischer, I., Peters, D., Rasch, R. and Pociuli, S. Replication of porcine circovirus: induction by glucosamine and cell cycle dependence. Arch. Virol. 1987, 96, 39-57
35 Meehan, B. M., Creelan, J. L., McNulty, M. S. and Todd, D. Sequence of porcine circovirus DNA: affinities with plant circoviruses. J. Gen. Virol. 1997, 78, 221-227
36 Clark, E. G. Post-weaning multisystemic wasting syndrome. Proc. Am. Assoc. Swine Pract. 1997, 28, 499-501
37 Allan, G. M., McNeilly, F., Cassidy, J. P., Reilly, G. A., Adair, B., Ellis, W. A. and McNulty, M. S. Pathogenesis of porcine circovirus; experimental infections of colostrum deprived piglets and examination of pig foetal material. Vet. Microbiol. 1995, 44, 49-64
38 Larochelle, R., Antaya, M., Morin, M. and Magar, R. Typing of porcine circovirus in clinical specimens by multiplex PCR. J. Virol. Methods. 1999, 80, 69-75
39 Lyoo, K. S., Park, Y. H. and Park, B. K. Prevalence of porcine reproductive and respiratory syndrome virus, porcine circovirus type 2 and porcine parvovirus from aborted fetuses and pigs with respiratory problems in Korea. J. Vet. Sci. 2001, 2, 201-207
40 Hines, R. K. and Lukert, D. Porcine circovirus as a cause of congenital tremors in newborn pigs. Proc. Am. Assoc. Swine Pract. 1994, 25, 344-345
41 Lyoo, Y. S., Kim, J. H. and Park, C. K. Identification of porcine circoviruses with genetic variation from lymph nodes collected in pigs with PMWS. Korean J. Vet. Res. 1999, 39, 353-358
42 Ellis, J., Krakowka, S., Lairmore, M., Haines, D., Bratanich, A., Clark, E., Allan, G., Konoby, C., Hassard, L., Meehan, B., Martin, K., Harding, J., Kennedy, S. and McNeilly, F. Reproduction of lesions of postweaning multisystemic wasting syndrome in gnotobiotic piglets. J. Vet. Diagn. Invest. 1999, 11, 3-14