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Identification of porcine intestinal spirochetes isolated from Korea by NADH oxidase gene(nox) PCR-RFLP  

Kim, Tae-Jung (College of Veterinary Medicine, Chonnam National University)
Kim, Myoung-Hee (College of Veterinary Medicine, Chonnam National University)
Na, Young-Ran (College of Veterinary Medicine, Chonnam National University)
Jung, Suk-Chan (National Veterinary Research and Quarantine Service)
Lee, Jae-Il (College of Veterinary Medicine, Chonnam National University)
Publication Information
Korean Journal of Veterinary Research / v.44, no.4, 2004 , pp. 533-537 More about this Journal
Abstract
In this study, we performed a PCR-RFLP analysis of NADH oxidase gene(nox) for the characterization of porcine intestinal spirochetes isolated from Korea by the comparison with Brachyspira hyodysenteriae and B. pilosicoli reference strains. Eleven strains including four reference strains, B. hyodysenteriae B204, B234, B169, B. pilosicoli P43/6/78 and seven Korean isolates were used. PCR products of 939 bp were amplified using nox-specific primers and digested with two restriction enzymes, Bfm I and Dpn II. In study using Bfm I, both strains showed no difference in fragmented size(197 and 741 bp). When use Dpn II, B. hyodysenteriae showed two bands(209 and 684 bp), however B. pilosicoli showed a single band of 896 bp. Our results indicate that nox-specific PCR-RFLP could be used as a typing method of Brachyspira species and as an epidemiological method for identifying spirochetes isolated from swine.
Keywords
Brachyspira hyodysenteriae; Brachyspira pilosicoli;
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