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The prevalence of swine influenza viral antigens and serum antibodiesin Piglets in Jeju  

Jun, Yong-chul (Department of Veterinary Medicine, Cheju National University)
Yang, Hyoung-seok (Department of Veterinary Medicine, Cheju National University)
Yang, Na-yeoun (Department of Veterinary Medicine, Cheju National University)
Kim, Dae-yong (College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University)
Kim, Jae-hoon (Department of Veterinary Medicine, Cheju National University)
Bae, Jong-hee (Department of Veterinary Medicine, Cheju National University)
Publication Information
Korean Journal of Veterinary Research / v.44, no.3, 2004 , pp. 449-454 More about this Journal
Abstract
Ninety pigs under the age of 120-day-old requested at the diagnostic laboratory of animal diseases in Cheju National University were evaluated for the prevalence of tissue antigen and serum antibody to swine influenza virus (SIV). For histopathologic examination there was sampled at the consolidated area in cranioventral or dorsocaudal lobes of lungs. Lung tissues from all pigs were tested for the antigen of SIV type A by immunohistochemistry (IHC). Sera from 56 pigs were used for the antibody detection to SIV type A (subtype H1N1 and H3N2) by haemagglutinin inhibition test. Pneumonic lesions were observed in 72 cases (80%) of 90 pigs. Broncho-interstitial or interstitial pneumonia were more prevalent than suppurative or fibrinous bronchopneumonia. According to HI test, 46.4% of the tested sera showed seropositive. Positive sera were consisted with 5.3% for SIV H1N1, 28.6% for SIV H3N2, and 12.5% for both subtype to be tested, respectively. SIV antigens were detected in 51 cases(56.6%) of 90 pigs. Most SIV antigens were presented in the epithelium of the bronchi and bronchiole. Necrotizing bronchitis or bronchiolitis were observed in 28(31.1%) cases of all inspected pigs. These results suggested that SIV might be an important role to induce swine pneumonia in Jeju. Also IHC was very useful for the detection of SIV in the lung.
Keywords
swine influenza virus; pneumonia; immunohistochemistry; haemagglutinin inhibition test;
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1 Brown, I. H., Done, S. H., Spencer, Y. I., Cooley, W. A., Harris, P. A. and Alexander D. J. Pathogenicity of a swine influenza H1N1 virus antigenicallydistinguishable from classical and European strains. Vet. Record. 1993a, 132, 598-602
2 Gian, C. W. and Norman, F. C. Ultrastructural morphometric investigation of early lesions in the pulmonary alveolar region of pigs during experimental swine influenza infection. Am. J. Pathol. 1986, 122, 541-552
3 Lanza, I., Brown, I. H. and Paton, D. J. Pathogenicity of concurrent infection of pigs with porcine respiratory coronavirus and swine influenza virus. Res. Vet. Sci.1992, 53, 309-314
4 Renee, L., Rene, S. and Ronald, M. Immunohistochemical detection of swine influenza virus in porcine proliferative and necrotizing pneumonia cases from Quebec. Can. Vet. J. 1994, 35, 513-515
5 Schorr, E., Wentworth, D. and Hinshaw, V. S. Use of polymerase chain reaction to detect swine influenza virus in nasal swab specimens. Am. J. Vet. Res. 1994,55, 952-956
6 Stech, J., Xiong, X., Scholtissek, C. and Webster, R. G. Independence of evolutionary and mutational rates after transmission of avian influenza viruses to swine. J. Virology. 1999, 73, 1878-1884
7 Dea, S., Bilodeau, R., Sauvgeau, R., Montpetit, C. and Martineau, G. P. Antigenic variant of swine influenza virus causing proliferative and necrotizingpneumonia in pigs. J. Vet. Diagn. Invest. 1992, 4, 380-392
8 Barigazzi, G., Foni, E., Candotti, P. and Cataldi, M. Antigenic analysis of swine influenza viruses isolated in Italy from 1976 to 1995. p. 100, in Proceedings, The14th IPVS Congress, Bologna, Italy. 1996
9 Brown, I. H., Harris, P. A. and Alexander, D. J. Serological studies of influenza viruses in pigs in Great Britain 1991-2. Epidemiol. Infect. 1995, 114, 511-520
10 Lee, B. W., Bey, R. F., Baarsch, M. J., Morrison, R. B. and Freese, W. Determination of hemagglutination inhibition titers to influenza A virus in porcine sera by use of an enzyme-linked immunosorbent assay. Am. J. Vet. Res. 1993, 54, 1270-1276
11 Brown, I. H., Manvell, R. J., Alexander, D. J., Chakraverty, P., Hinshaw, V. S. and Webster, R. G. Swine influenza outbreaks in England due to a new H1N1 virus. Vet. Record. 1993b, 132, 461-462
12 Morin, M., Girad, C., Eiazhary, Y., Fajardo, R., Drolet, R. and Lagace, A. Severe proliferative and necrotizing pneumonia in pigs: A newly recognized disease. Can. Vet. J. 1990, 31, 837-839
13 Jubb, K. V. F., Kennedy, P. C. and Palmer, N. (ed), Pathology of domestic animals. 4th. ed., Vol. 2, Academic press, Inc. San Diego, 1997, 539-699
14 Christensen, G., Sorensen, V. and Mousing, J. (ed), Disease of swine. pp. 913-940, 8th ed. Iowa state university press. Ames, 1999
15 Murphy, B. R and Webster, R. G. (ed), Influenza viruses. In Fields Virology. 2nd ed. Raven Press, New York, 1990, 1091-1154
16 Yewdell, J. W., Frank, E. and Gerhard, W. Expression of influenza A virus internal antigens on the surface of infected P815 cells. J. Immunology. 1981,126, 1814-1819
17 Brown, T. T., Mengeling, W. L., Paul, P. S. and Pirtle, E. C. Porcine fetuses with pulmonary hypoplasia resulting from experimental swine influenza virusinfection. Vet. Pathology. 1980, 17, 455-468
18 Lee, B. W., Bey, R. F., Baarsch, M. J. and Larson, M. E. Class specific response to influenza A H1N1 infection in swine. Vet. Microbiology. 1995, 43, 241-250
19 류영수, 박최규, 김로미, 이창희, 최상호, 김성일, 배종희. 제주지역에 대한 돼지 주요 전염병의 혈청학적 역학조시. 대한수의학회지. 1997, 37, 756-772
20 Easterday, B. C and Van Reeth, K. (ed), Disease of swine, 8th ed., Iowa state university press. Ames, 1999, pp. 277-290
21 류영수, 김로미. 돼지 인플루엔자 바이러스의 혈청학적 역학조사 및 유전학적 분석. 대한수의학회지. 1998, 38, 53-63
22 Kay, R. M., Done, S. H. and Paton, D. J. Effect of sequential porcine reproductive and respiratory syndrome and swine influenza on the growth and performance of finishing pigs. Vet. Record. 1994, 135, 199-204
23 Pol, J. M. A., Van Leengoed, L. A. M. G., Stockhope, N., Kok, G. and Wensvoort, G. Dual infections of PRRSV/ influenza or PRRSV/Actinobacillus pleuropneumoniae in the respiratory tract. Vet. Microbiology. 1997, 55, 259-264
24 Ito, T., Kawaoka, Y., Vines, A., Ishikawa, H., Asai, T. and Kida, H. Continued circulation of reassortant H1N2 influenza viruses in pigs in Japan. Arch. Virol.1998, 143, 1773-1782