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Quantitative assay of glycosphingolipid and determination of binding affinity for Shigatoxin 2e in pig  

Dong, Bun-youn (Korea Green Cross Co.)
Kim, Sang-Hyun (Department of Pathobiology, Ontario Veterinary College, University of Guelph)
Cho, Hyun-Ho (National Veterinary Research and Quarantine Service)
Kim, Yeong-Il (National Veterinary Research and Quarantine Service)
Lee, Woo-won (Pusan Institute of Health and Environment)
Kim, Kon-Sup (College of Veterinary Medicine, Research Institute of Life Science, Gyeongsang National University)
Kang, Ho-Jo (College of Veterinary Medicine, Research Institute of Life Science, Gyeongsang National University)
Kim, Yong-Hwan (College of Veterinary Medicine, Research Institute of Life Science, Gyeongsang National University)
Publication Information
Korean Journal of Veterinary Research / v.44, no.2, 2004 , pp. 233-239 More about this Journal
Abstract
This study was designed to quantitative assay of cellular receptor globotriosyl ceramide($Gb_3$) and globotetraosyl ceramide($Gb_4$) and to determine binding affinity of the receptors for Shigatoxin 2e in difference with day olds of pig. The amount of $Gb_3$ and $Gb_4$ extracted from each swine tissue were detected in order of spleen, ileocecum, kidney, and jejunum. The amount of $Gb_3$ and $Gb_4$ extracted from each swine ileocecum and jejunum in different day old swine were detected in order of 24, 6, 98, 12 and 35, but the difference of amount was not significant. The binding affinity of $Gb_4$ extracted from ileocecum and jejunum with different day old swine for Stx2e were shown that the extracts of 24, 12, and 35 days old swine were higher significantly than that of 6 and 98 days old swine.
Keywords
Globotriosyl ceramide; globotetraosyl ceramide; Shigatoxin 2e; receptor binding affinity;
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1 Basta, M., Karmali, M. A. and Lingwood, C. A. Sensitive receptor specified enzyme-linked immunosorbent assay for Escherichia coli verotoxin. J. Clin. Microbiol. 1989, 27, 1617-1622
2 Frank, M. A., Jorsal, E. A., Ahrens, P., Jensen, N. E. and Meyling, A. Molecular characterization of Escherichia coli strains isolated from pigs with edemadisease, J. clin. Microbiol. 1997, 35, 20-24
3 Macleod D. L. and Gyles, C. L. Purification and characterization of an Escherchia coli shiga-like toxin II varient. Infect. Immun. 1990, 1232-1239
4 Boyd, B., Tyrrell, G., Maloney, M., Gyles, C., Brunton, J. and Lingwood, C. A. Alteration of the glycolipid binding specificity of the pig edema toxin from globotetraosyl to globotriaosyl ceramide alters In Vivo tissue targetting and results in a verotoxin I-like disease in pigs. J. Exp. Med. 1993, 177, 1745-1753
5 Makita, A. and Yamakawa, T. Biochemistry of organ glycolipid: I. Ceramide oligosaccharides of human, equine, and bovine spleens. J. Biochem. 1962, 51, 124-133
6 Hughes, A. K., Ergonul, A., Stricklett, P. K. and Kohan, D. E. Molecular basis for high renal cell sensitivity to the cytotoxic effect of shigatoxin-1: Upregulation of globotriosylceramide expression. J. Am. Soc. Nephrol. 2002, 13, 2239-2245
7 Methyiapun, S., Pohlenz, J. F. L. and Bertschinger, H. U. Ultrastructure of the intestinal mucosa in pigs experimentally inoculated with an edema diseaseproducing strain of Escherichia coli (O139:K12:H1). Vet. Pathol. 1984, 21, 516-520
8 Ergonul, Z., Clyton, F., Fogo, A. B. and Kohan, D. E. Shigatoxin-1 binding and receptor expression in human kidney do not change with age. Pediatr.Nephrol. 2003, 18, 246-253
9 Boyd, B. and Lingwood, C. A. Verotoxin receptor glycolipid in human renal tissue. Nephron. 1989, 51, 207-210
10 Endo, Y., Tsurugi, K., Yutsudo, T., Takeda, Y., Ogusawara, T. and Igarashi, K. Site of a verotoxin (VT2) from Escherichia coli O157:H7 and of Shigatoxin on eucaryotic ribosome. Eur. J. Biochem. 1988, 171, 45-50
11 Ling, H., Pannus, N. S., Boodhoo, A., Armstrong, G. D., Clark, C. G., Burn, J. L. and Read, R. J. A mutant shiga-like toxin II2 bound to its receptor Gb(3)structure of a group II shiga-like toxin with altered binding specificity. Structure Fold Des. 2000, 8, 253-264
12 Lingwood, C. A. Role of verotoxin receptors in pathogenesis. Elsevier Science. 1996, 4, 147-152
13 Folch, J., Lees, M. and Sloane-Stanley, G. A. A simple method for the isolation and purification of total lipids from animal tissues. J. Biol. Chem. 1957, 226,497-521
14 Endo, Y. and Tsurugi, K. RNA N-glycosidase activity of ricin A chain. J. Biol. Chem. 1987, 262, 8128-8130
15 김용환, Lingwood, C. A. Verotoxin 생성 대장균의 병원성에 관한 연구. 한국수의공중보건학회지. 1992, 16, 215-221
16 Samuel, J. E., Perera, L. P., Ward, S., O'Brien, A. D., Ginsburg, V. and Krivan, H. C. Comparison of the glycolipid receptor specificities of the Shiga-liketoxin type II and Shiga-like toxin type II variants. Infect. Immun. 1990, 58, 611-618
17 MacLeod, D. L., Gyles, C. L. and Wilcock, B. P. reproduction of edema disease of swine with purified shiga-like Toxin II Variant. Vet. Pathol. 1991, 28, 66-73
18 Waddell, T. E., Lingwood, C. A. and Gyles, C. L. Interaction of verotoxin 2e with pig inetestine. Infect. Immun. 1996, 64, 1714-1719
19 Frydendahl, K. Prevalence of serogroups and virulence in Escherichia coli associated with postweaning diarrhea and edema disease in pigs and a comparison of diagnostic approaches. Vet. Microbiol. 2002, 85, 169-182
20 Moxley R. A. Edema diease. Vet. North Am. Food Anim. Pract. 2000, 16, 175-185
21 Karp, G. In Cell Biology. p.145, McGraw-Hill, New York. 1984