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Study on the quantitation of moxidectin by fluoroscence derivatization and it's residual after subcutaneously injection in pigs  

Jang, Beom-su (College of Veterinary Medicine, Chungnam National University)
Lim, Jong-hwan (College of Veterinary Medicine, Chungnam National University)
Park, Byung-kwon (College of Veterinary Medicine, Chungnam National University)
Kim, Min-Kyu (College of Veterinary Medicine, Seoul National University)
Yun, Hyo-in (College of Veterinary Medicine, Chungnam National University)
Publication Information
Korean Journal of Veterinary Research / v.44, no.1, 2004 , pp. 23-28 More about this Journal
Abstract
We established a new method to analyze moxidectin using high performance liquid chromatography(HPLC) with fluorescence derivatization in order to obtain its residual profiles in biological samples. Recovery of moxidectin in tissue was 62% at 10 ppb. Average detection reproducibility in terms of coefficience variation was 4.47% at 0.32 to 10 ppb. Residual of moxidectin was studied in 44 Yorkshire-Landrace mixed bred male pigs administered subcutaneously 0, 200, or $800{\mu}g/kg$ body weight (BW) Residual profiles of moxdectin in blood, muscle, liver, kidney and fat of pigs were described. The concentration of the moxidectin in liver after administration of moxidectin was the highest among the tissues examined. Moxidectin in liver after administration of moxidectin as $200{\mu}g/kg$ BW was declined from $10.0{\pm}3.7ng/g$ at 10 day post administration to $0.5{\pm}0.3ng/g$ level at 40 day post administration. Residual levels of moxidectin in all samples were estimated to fall below the limit of quantitation (0.32 ng/ml) after 50 day after treatment of $200{\mu}g/kg$. Moxidectin showed no abnormal observations in all the clinical findings at any concentrations under these experimental conditions. In conclusion, this analysis method by HPLC after fluorescence derivatization was very effective for the detection of moxidectin in biological samples. We suggest that 50-day is safe enough for the withdrawal time of moxidectin in pigs, following the recommendation dose by the manufacturer.
Keywords
fluorescence derivatization; HPLC; moxidectin; residual concentration;
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1 Bowman, D. D., Parsons, J. C., Grieve, R. B. and Hepler, D. I. Effects of milbemycin on adult Toxocara canis in dogs with experimentally induced infection. Am. J. Vet. Res. 1988, 49, 1186-1189
2 Burg, R. W., Miller, B. M., Baker, E. E., Birnbaum, J., Currie, S. A., Hartman, R., Kong, Y. L., Monaghan, R. L., Olson, G., Putter, I., Tunac, J. B.,Wallick, H., Stapley, E. O., Oiwa, R. and Omura, S. Avermectins, new family of potent anthelmintic agents: producing organism and fermentation. Antimicrob. Agents Chemother. 1979, 15, 361-367
3 Shoop, W. L., Mrozik, H. and Fisher, M. H. Structure and activity of avermectins and mibemycines in animal health. Vet. Parasitol. 1995, 59, 139-156
4 de Montigny, P., Shim, J. S. and Pivnichny, J. V. Liquid chromatographic determination of ivermectin in animal plasma with trifluoroacetic anhydride and Nmethyl- imidazole as the derivatization reagent. J. Pharm. Biomer. Anal. 1990, 8, 507-511
5 Demeulenaere, D., Vercruysse, J., Dorny, P. and Claerebout, E. Comparative studies of ivermectin and moxidectin in the control of naturally acquiredcyathostome infections in horses. Vet. Rec. 1997, 141, 383-386
6 Sasaki, Y., Kitagawa, H., Okachi, H., Kajita, Y. and Ishihara, K. Clinical application of mibemycin D as a prophylactic agent against Dirofilaria immitisinfection in dogs: reaction in uninfected and infected dogs. Jpn. J. Vet. Sci. 1986, 48, 579-586
7 Rabel, S. R., Stobaugh, J. F., Heinig, R. and Bostick, J. M. Improvements in detection sensitivity for the determination of ivermectin in plasma using chromatographic techniques and laser-induced fluorescence detection with automated derivatization. J. Chromatogr. 1993, 617, 79-86
8 Takiguchi, Y., Mishima, H., Okuda, M., Terao, M., Aoki, A. and Fukuda, R. Milbemycins, a new family of macrolide antibiotics: fermentation, isolation andphysico-chemical properties. J. Antibiot. 1980, 33, 1120-1127
9 Vercruysse, J., Claerebout, E., Dorny, P., Demeulenaere, D. and Deroover, E. Persistence of the efficacy of pouron and injectable moxidectin against Ostertagia ostertagi and Dictyocaulus viviparus in experimentally infected cattle. Vet. Rec. 1997, 140, 64-66
10 Craven, J., Bjorn, H., Hennessy, D. R. and Friis, C. The effects of body composition on the pharmacokinetics of subcutaneously injected ivermectin and moxidectin in pigs. J. Vet. Pharmacol. Therap. 2002, 25, 227-232
11 Arena, J. P., Liu, K. K., Paress, P. S., Frazier, E. G., Cully, D. F., Mrozik, H. and Schaeffer, J. M. The mechanism of action of avermectins in Caenorhabditiselegans: correlation between activation of glutamatesensitive chloride current, membrane binding and biological activity. J. Parasitol. 1995, 81, 286-294
12 Choi, K. S., Yun, H. I., Park, S. C., Park, J. M. and Cho, J. H. Pharmacokinetics of abamectin after subcutaneous injection in pigs. Kor. J. Vet. Publ. Health. 1998, 22, 1-8.
13 McKellar, Q. A. and Benchaoui, H. A. Avermectins and milbemycins. J. Vet. Pharmacol. Therap. 1996, 19, 331-351