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Correlation between microsomal lipid peroxidation levels and drug metabolizing enzymes in rats on various ages  

Cho, Jong-Hoo (Bio-Safety Research Institute, College of Veterinary Medicine, Chonbuk National University)
Hwang, DaeWoo (SungBo Science Tech.)
Park, Sang-Youel (Bio-Safety Research Institute, College of Veterinary Medicine, Chonbuk National University)
Publication Information
Korean Journal of Veterinary Research / v.43, no.4, 2003 , pp. 579-585 More about this Journal
Abstract
The studies were carried out on the correlation between microsomal lipid peroxidation level and drug metabolizing enzyme activities in rat liver microsomal suspensions on various ages (2-week-old, 2, 4, 8, and 12-month-old). The lipid peroxidation levels of liver homogenates tended to be elevated in a 4-month-old rat livers, but it was a little decreased in 8 and 12-month-old rat livers. The lipid peroxidation levels of microsomal suspension was not shown any significant differences by ages. Lipid peroxidation levels and microsomal cytochrome P450 and NADPH-cytochrome c reductase activity showed a direct correlation (r=0.72 and r=0.64), respectively. The activities of cytochrome P450-dependent aminopyrine-N-demethylase and benzpyrene hydroxylase in rat liver microsomes were increased by ages up to 8-month-old rats and maintained in 12-month-old rats. The correlation between lipid peroxidation levels and these cytochrome-dependent enzyme activities showed a high direct correlation (r=0.97 and r=0.81), respectively.
Keywords
microsome; cytochrome P450; drug metabolizing enzymes;
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