Browse > Article

Mechanism of vasodilatation induced by substance P in isolated rabbit renal artery  

Kim, Joo-heon (College of Veterinary Medicine and Institute of Animal Medicine, Gyeongsang National University)
Jeon, Seok-cheol (Cardiac center Masan Samsung General Hospital)
Hong, Yonggeun (Department of Internal Medicine-Cardiology, University of Texas Southwestern Medical Center at Dallas)
Publication Information
Korean Journal of Veterinary Research / v.43, no.4, 2003 , pp. 573-578 More about this Journal
Abstract
The effects of removing the endothelium on the vasodilatory response to substance P, calcitonin gene-related peptide (CGRP), and vasoactive intestinal peptide (VIP) was examined in the isolated rabbit renal artery. The vasodilator response to substance P ($0.1{\mu}M$) was completely absent in vessels in which the endothelium had previously been removed. There was no significant difference in the vasodilatation produced in response to CGRP ($0.1{\mu}M$), or VIP ($0.1{\mu}M$) in the intact and removed-endothelium rabbit renal artery segments. L-NAME ($100{\mu}M$) significantly reduced the vasodilatory response to substance P ($0.1{\mu}M$). This inhibition was significantly attenuated when L-arginine (10 mM) was also present in the organ bath along with L-NAME ($100{\mu}M$). Indomethacin ($1{\mu}M$) did not significantly affect the vasodilatation produced in response to substance P ($0.1{\mu}M$). The inhibitory effect of L-NAME ($100{\mu}M$) and indomethacin ($1{\mu}M$) on the vasodilatory response to substance P ($0.1{\mu}M$) was not significantly different from that produced by L-NAME ($100{\mu}M$) alone. This study indicates that substance P induced vasodilatation via an endothelium-dependent mechanism in the isolated rabbit renal artery. It also established that CGRP and VIP induced vasodilatation by an endothelium-independent mechanism and substance P-induced vasodilatation is at least partially via NO.
Keywords
vasodilatation; nitric oxide; substance P; calcitonin gene-related peptide; vasoactive intestinal peptide; renal artery; rabbit;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Beny, J. L. and Brunet, P. C. Electrophysiological and mechanical effects of substance P and acetylcholine on rabbit aorta. J. Physiol (London), 1988, 398, 277-289
2 Boulanger, C., Hendrickson, H., Lorenz, R. R. and Vanhoutte, P. M. Release of different relaxing factors by cultured porcine endothelial cells. Circ. Res. 1989, 64, 1070-1078
3 Brizzolara, A. L. and Burnstock, G. Endothelium-dependent and endothelum-independent vasodilatation of the hepatic artery of the rabbit. Brit. J. Pharmacol. 1991. 103, 1206-1212
4 Furchgott, R. F. The role of the endothelium in the responses of vascular smooth muscle to drugs. Ann. Rev. Pharmacol. Toxicol, 1984, 24, 175-197
5 Kim, J. H., Kwun, J. K. and Kirn, Y. K. Relatiunship of action adenosine triphosphate and prostaglandin $F_2_\alpha$ on uterine smooth muscle motility in immature pig. Korean J. Physiol. 1988, 22, 31-39
6 Milner, R, Kirkpatrick, K. A., Ralevic, V., Toothill, V., Pearson, J. and Burnstock, G. Endothelial cells cultured from human umbilical vein release ATP, substance P and acetylcholine in response to increased flow. Proc R Soc Lond B, 1990, 241, 245-248
7 Needham, L., Cusack, N. J., Pearson, J. D. and Gordon, J. L. Characteristics of the P2-purinoceptor that mediates prostacyclin production by pig aortic endothelial cells. Eur. J. Pharmacol. 1987, 134, 199-209
8 Palmer, R. M., Rees, D. D., Ashton, D. S. and Moncada, S. L-arginine is the physiological precursor for the formation of nitric oxide in endothelium-dependent relaxation. Biochem. Biophys. Res. Commun. 1988, 153, 1251-1256
9 Radomski, M. W., Palmer, R. M. and Moncada, S. The antiaggregating properties of vascular endothelium: interactions between prostacyclin and nitric oxide. Brit. J. Pharmacol. 1987, 92, 639-646
10 Ralevis, V., Milner, R, Hiidlicka. 0., Kristek, F. and Burnstock, G. Substance P is release from the endothelium of normal and capsaicin treated rat hindlimb vasculature in vivo by increased flow. Circ. Res. 1990, 66, 1178-1183
11 Vanhoutte, P. M. and Rimele. T. J. Role of the endothelium in the control of vascular smooth muscle function. J. Physiol. 1982-1983, 78, 681-686
12 Angus, J. A. and Cocks, T. M. Endothelium-derived relaxing factor. Pharmacvol Ther, 1989, 41, 303-352
13 Peach, M. J., Loeb, A. L., Singer, H. A. and Saye, J. Endothelium-derived vascular relaxing factor. Hypertension, 1985, 7, 94-100
14 Forstermann, U., Hertting, G. and Neufgang, B. The role of endothelial and non-endothelial prostaglandins in the relaxation of isolated blood vessels of the rabbit induced by acetylcholine and bradykinin. Brit. J. Phannacol. 1986, 87, 521-532
15 DeMey, J. and Vanhoutte, P. M. Removal of endothelium and arterial reactivity to acetylcholine and adenine nucleotides. Proc. Brit. Pharmacol. Soc. 10-12 Sept C.46
16 Kim, J. H., Shim, C. S. and Jeon, S. C. Involvement of nitric oxide and prostanuid on the endothelium- dependent vasodilatation by acetylcholine in the isolated rabbit renal artery. Korean J. Vet. Res. 2001, 41. 299-304
17 Kitagawa, S., Yamaguchi, Y., Kunitoino, M., Sanioshima, E. and Fujiwara, M. NG-nilro-L-arginine-resistant endothelium-dependent relaxation induced by acetylcholine in the rabbit renal artery. Life Sci. 1994, 55, 491-498
18 Mayer, B., Schmidt, K., Humbert, P. and Bohme, E. Biosynthesis of endothelium-derived relaxing factor: a cytosolic enzyme in porcine aortic endothelial cells $Ca^2^+$-dependently converts L-arginine into an activator of soluble guanylate cyclase. Biochem. Biophys. Res. Commun. 1989, 164, 678-685
19 Beny, J. L., Brunet, P. C. and Huggel, H. Effect of mechanical stimulation, substance P and vasoactive intestinal pulypeptide on the electrical and mechanical activities of circular smooth muscle from pig coronary arteries contracted with acetylcholine: role of endothelium. Pharmacology, 1986. 33, 61-68
20 Cherry, P. D., Furchgott, R. W., Zawadzki, J. V., Jothianandan, Role of endothelial cells in relaxation of isolated arteries by bradykinin. Proc. Natl. Sci. USA, 1982, 79, 2106-2110
21 Loesch, A. and Burnstock, G. Ultrastructural localisation of serotonin and substance P in vascular endothelial cells of rat femoral and mesenteric arteries. Anat. Embryol. 1988, 178, 137-142
22 Palmer, R. M. and Moncada, S. A novel citrulline-forming enzyme implicated in the formation of nitric oxide by vascular endothelial cells. Biochem. Biophys. Res. Commun. 1989, 158, 348-352
23 Schmidt, H. W., Klein, M. M., Niroomand, F. and Bohme, E. Is arginine a physiological precursor of endothelium-derived nitirc oxide? Eur. J. Pharmacol. 1988, 148, 293-295
24 Vane, J. R. Inhibition of prostaglandin synthesis as a mechanism of action of aspirin like drugs. Nat. New Biol. 1971, 231, 232-235
25 Palmer, R. M., Ferridge, A. G. and Moncada, S. Nitric oxide release accounts for the biological activity of endothelium-derived relaxing factor. Nature, 1987, 327, 524-526
26 Rees, D. D., Palmer, R. M., Schulz, R., Hodson, H. F. and Moncada, S. Characterization of three inhibitors of endothelial nitric oxide synthase in vitro and in vivo. Brit. J. Pharmacol. 1990, 101, 746-752
27 Furchgott, R. F. Role of the endothelium in responses of vascular smooth muscle. Circ. Res. 1983, 53, 557-573
28 Ignarro, L. J., Wood, K. S. and Byrns, R. E. Pharmacological and biochemical properties of EDRF: evidence that EDRF is closely related to nitric oxide (NO) radical, Circulation, 1986, 74, 287-299
29 Herbaczynska-Cegro, K. and Vane, J. R. Contribution of intrarenal generation of prostaglandins to autoreeulation of renal blood flow in the dog. Circ. Res. 1973, 32, 428-436
30 Ignarro, L. J., Byrns, R. E., Buga, G. M. and Wood, K. S. Endothelium-derived relaxing factor from pulmonary artery and vein possesses pharmacologic and chemical properties identical to those of nitric oxide radical. Circ. Res. 1987, 61, 866-879
31 Peach, M. J., Singer, H. A. and Loeb, A. L. Mechanisms of endothelium-dependent vascular smooth muscle relaxation. Biochem. Pharmacol. 1985, 34, 1867-1874
32 Furchgott, R. F. and Zawadzki, J. V. The obligatory role of endothelial cells in the relaxation of arterial smooth muscle by acetylcholine. Nature 1980, 288, 373-376
33 Furchgott, R. F., Khan, M. T, Joithianandan, D., Evidence supporting the proposal that endothelium- derived relaxing factor is nitric oxide. Thrombosis Res. Suppl. 1987, VII 5