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Extracellular signal regulated kinases in the spinal cord of rats with experimental autoimmune encephalomyelitis  

Ahn, Mee-jung (Department of Veterinary Medicine, College of Agriculture and Life Sciences, Cheju National University)
Heo, Seung-dam (Department of Veterinary Medicine, College of Agriculture and Life Sciences, Cheju National University)
Jee, Young-heun (Department of Veterinary Medicine, College of Agriculture and Life Sciences, Cheju National University)
Joo, Hong-gu (Department of Veterinary Medicine, College of Agriculture and Life Sciences, Cheju National University)
Lee, Yong-duk (Department of Veterinary Medicine, College of Agriculture and Life Sciences, Cheju National University)
Sim, Ki-Bum (Department of Neurosurgery, College of Medicine, Cheju National University)
Shin, Tae-kyun (Department of Veterinary Medicine, College of Agriculture and Life Sciences, Cheju National University)
Publication Information
Korean Journal of Veterinary Research / v.43, no.4, 2003 , pp. 525-529 More about this Journal
Abstract
The phosphorylation of extracellular signal-regulated kinases (p-ERK) in the spinal cord of rats with acute monophasic experimental autoimmune encephalomyelitis (EAE) was studied using immunohistochemistry and treatment with inhibitor. P-ERK is constitutively expressed in glial cells in the normal spinal cord. In EAE, some inflammatory cells in the subarachnoid space were positive for p-ERK at the early stage, and its immunoreactivity declined when those cells infiltrated the parenchyma at the peak stage. In a blocking experiment using its inhibitor, the intravenous administration of PD98059 from day 7 to 13 post-immunization did not modulate EAE paralysis. Considering the results, we postulate that intravenous administration of PD98059 is not effective in ameliorating EAE paralysis, although many inflammatory cells express ERK in the subarachnoid space.
Keywords
autoimmune encephalomyelitis; extracellular signal-regulated kinases; spinal cord;
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