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Antimicrobial effects of β-lactamase inhibitor/β-lactam antibiotics on staphylococcal mastitis  

Lim, Suk-Kyung (National Veterinary Research & Quarantine Service)
Lim, Jae-Hhyang (Research Institute of Veterinary Medicine, Chonnam National University)
Joo, Yi-Seok (National Veterinary Research & Quarantine Service)
Moon, Jin-San (National Veterinary Research & Quarantine Service)
Lee, Ae-Ri (Sangmyung University)
Koh, Hong-Bum (Research Institute of Veterinary Medicine, Chonnam National University)
Publication Information
Korean Journal of Veterinary Research / v.43, no.1, 2003 , pp. 113-120 More about this Journal
Abstract
The antimicrobial effect of ${\beta}$-lactam antibiotics, which had ${\beta}$-lactamase inhibitor activity, on Staphylococcus aureus isolated from mastitis was investigated in this study. Out of 166 isolates, 99 isolates (59.6%) produced ${\beta}$-lactamase, and 98 isolates of 99 were ${\beta}$-lactamase positive in above $12.5{\mu}g/m{\ell}$ MIC of penicillin. In the providence distribution, ${\beta}$-lactamase production rate of 4 providence, Gangwon, Gyeonggi, Chungcheong, and Jeolla was 100%, 65.7%, 58.8%, and 50.0%, respectively. Antibiotic activities of ${\beta}$-lactam antibiotics against lactamase positive isolates also were investigated. Antimicrobial effects of ampicillin/sulbactam or amoxicillin/clavulanic acid treated group were better than ampicillin or amoxicillin treated group. In antimicrobial effects on intracellular S aureus, there was no difference 1 hour and 4 hour treatment in control, ampicillin, and amoxicillin group, but in 18 hours treatment, ampicillin/sulbactam or amoxicillin/clavulanic acid had a better effect than ampicillin or amoxicillin (p<0.05).
Keywords
${\beta}$-lactam; ${\beta}$-lactam inhibitor; lactamase; Staphylococcus aureus; mastitis; antimicrobial;
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