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Immunohistochemical study of the pancreatic endocrine cells in the ICR mice  

Ku, Sae-kwang (Pharmacology & Toxicology Lab., Central Research Laboratories, Dong-Wha Pharm. Ind. Co.)
Lee, Hyeung-sik (Department of Biology, Faculty of Natural Sciences, Kyungsan University)
Lee, Jae-hyun (Department of Histology, College of Veterinary Medicine, Kyungpook National University)
Publication Information
Korean Journal of Veterinary Research / v.42, no.1, 2002 , pp. 21-28 More about this Journal
Abstract
The regional distribution and relative frequency of the pancreatic endocrine cells in the ICR mouse were studied by immunohistochemical (PAP) method using four types of specific antisera against insulin, glucagon, somatostatin and human pancreatic polypeptide (PP). The pancreas of mice could be divided into three portions; pancreatic islets, exocrine and pancreatic ducts. Pancreatic islets, furthermore, were subdivided into three regions (central, mantle and peripheral region) according to their located types of immunoreactive cells. In the pancreatic islet portions, insulin-immunoreactive cells were located in the central and mantle regions but most of somatostatin-, glucagon- and PP-immunoreactive cells were detected in the mantle and peripheral regions with various frequencies. In addition, PP-immunoreactive cells were also found in the central regions of pancreatic islets of ICR mouse. In the exocrine portions, all four types of immunoreactive cells were demonstrated in the ICR mouse. In the pancreatic duct portions, insulin- and glucagon-immunoreactive cells were situated in the epithelial lining of ICR mouse with a few and rare frequencies, respectively. In addition, rare PP-immunoreactive cells were also demonstrated in the subepithelial regions of the pancreatic duct. However, no somatostatin-immunoreactive cells were demonstrated.
Keywords
ICR mouse; pancreatic endocrine cell; immunohistochemistry;
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