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http://dx.doi.org/10.5667/tang.2018.0015

Aristolochia ringens extract ameliorates oxidative stress and dyslipidaemia associated with streptozotocin-induced hyperglycaemia in rats  

Sulyman, Abdulhakeem Olarewaju (Department of Biochemistry, Kwara State University)
Akolade, Jubril Olayinka (Biotechnology Advanced Research Centre, Sheda Science and Technology Complex)
Aladodo, Raliat Abimbola (Department of Biochemistry, Kwara State University)
Ibrahim, Rasheed Bolaji (Department of Biochemistry, Kwara State University)
Na'Allah, Asiat (Department of Biological Sciences, Al-Hikmah University)
Abdulazeez, Azeemat Titilola (Department of Biological Sciences, Al-Hikmah University)
Publication Information
CELLMED / v.8, no.3, 2018 , pp. 12.1-12.7 More about this Journal
Abstract
The study was designed to assess antioxidant and antidyslipidaemic effects of terpenoid-rich extract from the root of Aristolochia ringens V. Hyperglycemia-induced oxidative stress and dyslipidemia were established in rats by single intraperitoneal administration of 65 mg/kg bw streptozotocin. Based on therapeutic dose determined in previous study, streptozotocin-induced rats were orally administered with 75 and 150 mg/Kg bw of A. ringens extract for 14 days. Total protein, serum lipid profiles and biomarkers of oxidative stress in liver and kidney of the experimental rats were determined. Atherogenic and cardiovascular disease risk indices were computed. Streptozotocin-induced hyperglycaemia significantly (p < 0.05) decreased activities of superoxide dismutase, catalase and glutathione transferase as well as the amount of reduced glutathione in both tissues indicating oxidative stress induced kidney and liver injury due to glucotoxicity. In comparison to non-treated hyperglycaemic rats, activities of the antioxidant enzymes and concentration of glutathione-H were significantly (p < 0.0001) increased, whereas malondialdehyde was reduced in the tissues of rats treated with both 75 and 150 mg/Kg bw of the extract. The extract also caused significant (p < 0.001) reduction in elevated levels of total cholesterol, triglycerides and low density lipoprotein-cholesterol levels, whereas concentration of the attenuated high density lipoprotein-cholesterol was increased in serum of the treated rats. Reduced atherogenic and cardiac risk indices were projected for the A. ringens extract-treated groups. Results from this study showed that extract from A. ringens root was rich in terpenoids and may reduce risks of complications associated with hyperglycemia-induced oxidative stress and dyslipidemia.
Keywords
Aristolochia ringens; diabetes; dyslipidemia; hyperglycaemia; oxidative stress;
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1 Kalinina E, Chernov N and Novichkova M. Role of glutathione, glutathione transferase, and glutaredoxin in regulation of redox-dependent processes. Biochemistry (Moscow), 2014; 79(13): 1562-1583.   DOI
2 Marfella R, D'Onofrio N, Sirangelo I, Rizzo MR, Capoluongo MC, Servillo L, Paolisso G and Balestrieri ML. Polyphenols, Oxidative Stress, and Vascular Damage in Diabetes Diabetes: oxidative stress and dietary antioxidants. Academic Press; 2014.
3 Marrelli M, Cristaldi B, Menichini F and Conforti F. Inhibitory effects of wild dietary plants on lipid peroxidation and on the proliferation of human cancer cells. Food and Chemical Toxicology, 2015;86:16-24.   DOI
4 Misra HP and Fridovich I. The role of superoxide anion in the autoxidation of epinephrine and a simple assay for superoxide dismutase. Journal of Biological Chemistry, 1972;247(10):3170-5.
5 Nazaruk J and Borzym-Kluczyk M. The role of triterpenes in the management of diabetes mellitus and its complications. Phytochemistry Reviews, 2015;14(4):675-690.   DOI
6 Nishikawa T, Edelstein D, Du XL, Yamagishi Si, Matsumura T, Kaneda Y, Yorek MA, Nishikawa T, Osho I and Lajide L. Antitrypanosomal activity of Aristolochia ringens against Trypanosoma congolense infection in mice. Journal of Pharmacognosy and Phytotherapy. 2014;6(1): 1-3.   DOI
7 Rani MP and Padmakumari KP. HPTLC and reverse phase HPLC methods for the simultaneous quantification and in vitro screening of antioxidant potential of isolated sesquiterpenoids from the rhizomes of Cyperus rotundus. J Chromatogr B Analyt Technol Biomed Life Sci, 2012;904:22-28.   DOI
8 Ruth AF, Olaide, A. O. and Oluwatoyin, S. M. The aqueous root extract of Aristolochia ringens (Vahl.) Aristolochiaceae inhibits chemically-induced inflammation in rodents. Pak. J. Pharm. Sci, 2014;27(6), 1885-1889.
9 Saljoughian S, Roohinejad S, Bekhit AE-DA., Greiner R, Omidizadeh A, Nikmaram N and Mousavi Khaneghah A. The effects of food essential oils on cardiovascular diseases: A review. Critical Reviews in Food Science and Nutrition, 2018;58(10)1688-1705.   DOI
10 Silou T, Nienzy JP, Nsikabaka S, Loumouamou AN and Bikindou K. Extraction of Elionurus Hensii K. Schum Essential Oil in a Domestic Scale in the "Plateau Des Cataractes" (Congo-Brazzaville). International Journal of Engineering Science Technologies, 2017;2(1).
11 Abdulrazaq NB, Cho MM., Win NN., Zaman R. and Rahman MT. Beneficial effects of ginger (Zingiber officinale) on carbohydrate metabolism in streptozotocin-induced diabetic rats. British Journal of Nutrition, 2012;108(7):1194-1201.   DOI
12 Adeyemi O, Aigbe F. and Badru O. The antidiarrhoeal activity of the aqueous root extract of Aristolochia ringens (Vahl.) Aristolochiaceae. Nigerian quarterly journal of hospital medicine, 2012;22(1):29-33.
13 Aigbe FR., Munavvar ASZ., Rathore H., Eseyin O, Pei YP, Akhtar S, Chohan A, Jin H, Khoo J, Tan S and et al. Alterations of haemodynamic parameters in spontaneously hypertensive rats by Aristolochia ringens Vahl. (Aristolochiaceae). Journal of Traditional and Complementary Medicine, 2018;8(1):72-80   DOI
14 Bandyopadhyay U, Das D and Banerjee RK. Reactive oxygen species: Oxidative damage and pathogenesis. Current science, 1999;77(5): 658-666.
15 Akindele AJ, Wani Z, Mahajan G, Sharma S, Aigbe FR, Satti N, Adeyemi OO and Mondhe DM. Anticancer activity of Aristolochia ringens Vahl. (Aristolochiaceae). Journal of Traditional and Complementary Medicine, 2015;5(1):35-41.   DOI
16 Almuaigel MF, Seif MA, Albuali HW, Alharbi O and Alhawash A. Hypoglycemic and hypolipidemic effects of aqueous extract of phaseolus vulgaris pods in streptozotocin-diabetic rats. Biomedicine & Pharmacotherapy, 2017;94:742-746.   DOI
17 Armstrong D and Al-Awadi F. Lipid peroxidation and retinopathy in streptozotocin-induced diabetes. Free Radical Biology and Medicine, 1991;11(4):433-436.   DOI
18 Szkudelski T. Streptozotocin-nicotinamide-induced diabetes in the rat. Characteristics of the experimental model. Experimental Biology and Medicine, 2012;237(5):481-490.   DOI
19 Sulyman AO, Akolade JO, Na’Allah A, Aladodo RA and Jamiu HO. Effect of Administration of Root Ethanolic Extract of Aristolochia Ringens on the Liver Functional Indices of Male Wistar Rats. Iranian Journal of Toxicology, 2017;11(1):55-58.   DOI
20 Szkudelski T. The mechanism of alloxan and streptozotocin action in B cells of the rat pancreas. Physiological research, 2001;50(6):537-546.
21 Szkudelski T and Szkudelska K. Streptozotocin induces lipolysis in rat adipocytes in vitro. Physiological research, 2002;51(3):255-260.
22 Tangvarasittichai S. Oxidative stress, insulin resistance, dyslipidemia and type 2 diabetes mellitus. World journal of diabetes, 2015;6(3):456.   DOI
23 Kostalova D, Hrochova V, Pronayova N and Lesko J. Constituents of Aristolochia-clematitis L. Chemical Papers, 1991; 45(5):713-716.
24 Friedewald WT, Levy RI and Fredrickson DS. Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clinical chemistry, 1972;18(6):499-502.
25 Draper HH, Squires EJ, Mahmoodi H, Wu J, Agarwal S and Hadley M. A comparative evaluation of thiobarbituric acid methods for the determination of malondialdehyde in biological materials. Free Radical Biology and Medicine, 1993;15(4):353-363.   DOI
26 Edelstein D, Du XL, Yamagishi S, Matsumura T, Kaneda Y, Yorek MA, Oates PJ and et al. Normalizing mitochondrial superoxide production blocks three pathways of hyperglycaemic damage. Nature, 2000; 404(6779):787-790.   DOI
27 Ellman GL. Tissue sulfhydryl groups. Archives of biochemistry and biophysics, 1959;82(1):70-77.   DOI
28 Grassmann J. Terpenoids as plant antioxidants. Vitamins & Hormones, 2005;72:505-535.
29 Lowry OH, Rosebrough NJ, Farr AL and Randall RJ. Protein measurement with the Folin phenol reagent. Journal of biological chemistry, 1951;193(1):265-275.
30 Loumouamou AN, Bikindou K, Bitemou E, Chalard P, Silou T and Figueredo G. Optimization of the extraction of the pmenthadienol isomers and aristolone contained in the essential oil from Elyonurus hensii using a 23 full factorial design. Food Science & Nutrition, 2017;5(3):784-792.   DOI
31 Madhuri K and Naik PR. Ameliorative effect of borneol, a natural bicyclic monoterpene against hyperglycemia, hyperlipidemia and oxidative stress in streptozotocin-induced diabetic Wistar rats. Biomedicine & Pharmacotherapy, 2017;96:336-347.   DOI
32 Sindhu RK, Koo JR, Roberts CK and Vaziri ND. Dysregulation of hepatic superoxide dismutase, catalase and glutathione peroxidase in diabetes: response to insulin and antioxidant therapies. Clinical and experimental hypertension, 2004;26(1):43-53.   DOI
33 Sinha AK. Colorimetric assay of catalase. Analytical biochemistry, 1972;47(2):389-394.   DOI
34 Sulyman A, Akolade J, Sabiu S, Aladodo R and Muritala H. Antidiabetic potentials of ethanolic extract of Aristolochia ringens (Vahl.) roots. Journal of ethnopharmacology, 2016;182:122-128.   DOI
35 Institute for Laboratory Animal Research(U.S.), National Academies Press (U.S.). Guide for the Care and Use of Laboratory Animals (8th edition). National Academies Press; 2010.
36 Grassmann J, Hippeli S and Elstner EF. Plant's defence and its benefits for animals and medicine: role of phenolics and terpenoids in avoiding oxygen stress. Plant Physiology and Biochemistry, 2002;40(6):471-478.   DOI
37 Habig WH, Pabst MJ, Fleischner G, Gatmaitan Z, Arias IM and Jakoby WB. The identity of glutathione S-transferase B with ligandin, a major binding protein of liver. Proceedings of the National Academy of Sciences, 1974;71(10):3879-3882.   DOI
38 Huber H. Aristolochiaceae. In: Kubitzki K., Rohwer J.G., Bittrich V. editors. Flowering Plants.Dicotyledons. The Families and Genera of Vascular Plants, vol 2. Springer, Berlin; 1993. p.129-137