[KSCI] Korea Science Citation Index Service

http://dx.doi.org/10.7745/KJSSF.2016.49.1.036

The Effects of Calcium Nutrition on the Activities of Lactate Dehydrogenase, Alcohol Dehydrogenase and Other Enzymes in Melon (Cucumis melo L.) Seedlings Subjected to Flooding

Lee, Chang-Hee (School of Applied of Life Science, Kyungpook National University)
Park, Man (School of Applied of Life Science, Kyungpook National University)
Kang, Sang-Jae (School of Applied of Life Science, Kyungpook National University)

Publication Information

Korean Journal of Soil Science and Fertilizer / v.49, no.1, 2016 , pp. 36-43 More about this Journal

Abstract

With transient flooding followed by poor or slow drainage plant roots may become reduction conditions because the root zone was fully filled with water. This study was examined the effects of calcium treatment in the early growth stage on biochemical changes in leaves and roots of melon (Cucumis melo L.) seedlings kept under flooding condition for 72 h. The activities of lactate dehydrogenase more gradually enhanced in the roots than those of leaves of melon seedlings treated with calcium. The activities of alcohol dehydrogenase associated with alcohol fermentation under low oxygen conditions continuously increased in the leaves and roots of seedlings untreated with calcium under flooding at least 72 h but those was constant within at least 12 h in treated with calcium. These results showed that calcium supplying in the early growth stage mitigated alcohol fermentation of melon seedlings kept under flooding condition for 72 h. Activities of nitrate reductase and acid phosphatase in the leaves and roots of seedlings in treated with calcium somewhat higher than those of non-treated with calcium. The activities of sucrose phosphate synthase and fructose-1,6-bisphosphatase of leaves of seedlings in treated with calcium more higher than those of non-treated with calcium. These results indicated that calcium nutrition mitigate the reduction of activities of some enzymes of melon seedling kept under flooding condition for 72 h.

Keywords

Alcohol dehydrogenase; Calcium nutrition; Flooding; Lactate dehydrogenase; Melon seedling;

Citations & Related Records

Reference

1	Andrew, D.L., M.C. Drew, J.R. Johnson, and B.G. Cobb. 1994. The response of maize seedlings of different ages to hypoxic and anoxic stress. Plant Physiol. 105:53-60. DOI
2	Beck, E. and P. Ziegler. 1989. Biosynthesis and degradation of starch in higher plants. Annu. Rev. Plant Physio. Plant Mol. Biol. 40:95-118. DOI
3	Biemelt, S., U. Keetman, and G. Albrecht. 1998. Reaeration following hypoxia of anoxia lead to activation of the antioxidative defense system in roots of wheat seedlings. Plant Physiol. 116:651-658. DOI
4	Bozzo, G.G., K.G. Raghothama, and W.C. Plaxton. 2004. Structural ad kinetic properties of a novel purple acid phosphatase from phosphate-starved tomato (Lycopersicon esculentum) cell culture. Biochem. J. 377:419-428. DOI
5	Bradford, M.M. 1976. A rapid and sensitive method for the quantitation of microquantities of protein utilizing the principle of protein-dye binding. Anal. Biochem., 72:248-254. DOI
6	Bush, D.S. 1996. Effects of gibberellic acid and environmental factors on cytosolic calcium in wheat aleurone cells. Planta 199:89-99.
7	Chang, W.P., L. Hunag, M. Shen, C. Webster, A. Burlingame, and J.K.M. Roberts. 2000. Patterns of protein synthesis and tolerance of anoxia in root tips of maize seedlings acclimated to a low oxygen environment, and identification of proteins by mass spectrometry. Plant Physiol. 122:295-318. DOI
8	Chung, H.-J., and R.J. Ferl. 1999. Arabidopsis alcohol dehydrogenase expression in both shoots and roots is conditioned by root growth environment. Plant Physiol. 121:429-436. DOI
9	Drueckes, P., R. Schinzel, and D. Palm. 1995. Photometric microtiter assay of inorganic phosphate in the presence of acid-labile organic phosphate. Anal. Biochem. 230:173-177. DOI
10	Drew, M.C. 1997. Oxygen deficiency and root metabolism: Injury and acclimation under hypoxia and anoxia. Annu. Rev. Plant Physiol. and Plant Mol. Biol. 48:223-250. DOI
11	Epstein, E., and A.J. Bloom. 2005. Ecology and Environmental Stress. p. 328-330, Mineral Nutrition of Plants: Principles and Perspective, 2 nd edition, Sinauer Associate, MA. USA.
12	Ferl, R., and B. Laughner. 1989. In vivo detection of the regulatory factor binding sites of Arabidopsis thaliana Adh. Plant Mol. Biol. 12:357-366.
13	Fukuda, T., F. Yokoyama, T. Nakamura, I-J. Song, T. Ito, T. Ochiai, A. Kanno, T. Kameya, and M. Maki. 2005. Molecular phylogeny and evolution of alcohol dehydrogenase (Adh) genes in legumes. BMC Plant Biol. 5:6-15. DOI
14	Grierson, W. 1999. Beneficial aspects of stress on plants. In: Handbook of Plant and crop stress, 2 nd edition, Pessarakli, M., ed. Dekker, New York. pp 1185-1198.
15	Johnson, J.R., B.G. Cobb, and M.C. Drew. 1994. Hypoxic induction of anoxia tolerance in roots of Adh null Zea mays L. Plant Physiol. 105:61-67. DOI
16	Hoffman, N.E., A.F. Bent, and A.D. Hanson. 1986. Induction of lactate dehydrogenase isozymes by oxygen deficit in barley root tissue. Plant Physiol. 82:658-663. DOI
17	Ismail, A.M., E.S. Ella, G.V. Vergara, and D.J. Mackill. 2009. Mechanisms associated with tolerance to flooding during germination and early seedling growth in rice (Oryza sativa). Annal. Bot. 103:197-209. DOI
18	Jeschke, W.D., E.A. Kirkby, A.D. Peuke, J.S. Pate, and W. Hartung. 1997. Effects of P deficiency on assimilation and transport of nitrate and phosphate in intact plants of caster bean (Ricinus communis L.). J. Exp. Bot. 48:75-91. DOI
19	Kato-Noguchi, H., K-N., and M. Morokuma. 2007. Ethanolic fermentation and anoxia tolerance in four rice cultivar. J Plant Physiol. 164:168-173. DOI
20	Kerr, P.S., S.C. Huber, and D.W. Israel. 1984. Effect of N-source on soybean leaf sucrose phosphate synthase, starch formation and whole plant growth. Plant Physiol. 75:483-488. DOI
21	Kyozuka, J. M. Olive, W. Peacock, E. Dennis, and K. Shimamoto. 1994. Promotor elements required for developmental expression of the maize Adh1 gene in transgenic rice. Plant Cell 6:799-810. DOI
22	Lefebvre, D.D., S.M.G. Duff, C.A. Fife, C. Julien-Inalsingh, and W.C. Plaxton. 1990. response to phosphate deprivation in Brassica nigra suspension cells. Plant Physiol. 93:504-511. DOI
23	Mann, A.F., D.P. Hucklesby, and E.J. Hewitt. 1979. Effect of aerobic and anaerobic conditions on the in vivo nitrate reductase assay in spinach leaves. Planta 146:83-89. DOI
24	Robinson, W.D., J.Park, H.T. Tran, H.A. Del Vecchio, S. Ying, J.L. Zins, K. Patel, T.D. McKnight, and W.C. Plaxton. 2012. The secreted purple acid phosphatase isozymes AtPAP12 and AtPAP26 play a pivotal role in extracellular phosphate scavenging by Arabidopsis thaliana. J. Exp. Bot 63:6531-6542. DOI
25	Matton, D.P., P. Constabel, and N. Brission. 1990. Alcohol dehydrogenase gene expression in potato following elicitor and stress treatment. Plant Mol. Biol. 14:775-783. DOI
26	Mayne, R.G. and P.J. Lea. 1984. Alcohol dehydrogenase in Hordeum vulgare: Changes in isoenzyme levels under hypoxia. Plant Sci. Lett. 37:73-78. DOI
27	Peng, H-P., C-S. Chan, M-C. Shih, and S.F. Yang. 2001. Signaling events in the hypoxic induction of alcohol dehydrogenase gene in Arabidopsis. Plant Physiol. 126:742-749. DOI
28	Rudd, J.J., and V.E. Fraklin-Tong. 2001. Unravelling response-specificity in $Ca^{+2}$ signalling pathways in plant cells. New Phytologist. 151:7-33. DOI
29	Rufty, T.W., P.S. Kerr, and S.C. Huber. 1983. Characterization of diurnal changes in activities of enzymes involved in sucrose biosynthesis. Plant Physiol. 73:428-433. DOI
30	Sach, M.M., M. Freeling, and R. Okimoto. 1980. The anaerobic proteins of maize. Cell 20:761-767. DOI
31	Sander, D., C. Brownlee, J.F. Harper. 1999. Communicating with calcium. The Plant Cell 11:691-706. DOI
32	Sedbrook, J.C., P.J. Kronebusch, G.G. Borisy, A.J. Trewavas, and P.H. Masson. 1996. Transgenic aequorin reveals organspecific cytosolic $Ca^{+2}$ responses to anoxia in Arabidopsis thaliana seedlings. Plant Physiol. 111:243-257. DOI
33	Taiz, L., and E. Zeiger. 2002. Photosynthesis: carbon reaction p. 145-170. Plant Physiology, 3rd edition, Sinauer Associates, Massachusettes, USA.
34	Solomon, L.P. and M.J. Barber. 1990. Assimilatory nitrate reductase: functional properties and regulation. Annu. Rev. Plant Biol. 41:224-253.
35	Subbaiah, C.C., J. Zhang, and M.M. Sach. 1994. Involvement of interacellular calcium in anaerobic gene expression an survival of maize seedlings. Plant Physiol. 105:369-376. DOI
36	Subbaiah, C.C., D.S. Bush, and M.M. Sachs. 1998. Mitochondrial contribution to the anoxic $Ca^{+2}$ signal in maize suspension-cultured cells. Plant Physiol. 118:759-771. DOI