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http://dx.doi.org/10.7745/KJSSF.2011.44.6.1181

Monitoring of Microorganisms in Commercial Liquid Pig Manures in Korea  

Lim, Seong-Mook (Chemical Safety Division, National Academy of Agricultural Science, RDA)
Lee, Ji-Ho (Chemical Safety Division, National Academy of Agricultural Science, RDA)
Go, Woo-Ri (Chemical Safety Division, National Academy of Agricultural Science, RDA)
Kunhikrishnan, Anitha (Chemical Safety Division, National Academy of Agricultural Science, RDA)
Kim, Won-Il (Chemical Safety Division, National Academy of Agricultural Science, RDA)
Publication Information
Korean Journal of Soil Science and Fertilizer / v.44, no.6, 2011 , pp. 1181-1184 More about this Journal
Abstract
Total aerobic bacteria, Esherichia coli O157:H7, and Salmonella spp. were examined in commercial liquid pig manures. Commercial liquid pig manures (n=33) were collected from muck joint resource recovery plant at April, June, August, October 2009, Korea. Total aerobic bacteria were incubated at $37^{\circ}C$ for 24-48 hrs, and quantified as a colony-forming unit (CFU) $mL^{-1}$. Analysis of Esherichia coli O157:H7 and Salmonella spp. were followed by Korean Food Standards Codex method. Colony of Salmonella spp. was confirmed by API kit and real time polymerase chain reaction (PCR). Total aerobic bacteria isolated from fermented commercial liquid pig manures ranged from 2.8 to $24.3{\times}10^4\;CFU\;mL^{-1}$. Esherichia coli O157:H7 was not detected, and Salmonella spp. showed the low detection frequency at only 1 sample. This study suggests that continuous monitoring in commercial liquid pig manures is required to improve the agricultural food through management of agricultural land contaminated with liquid pig manures.
Keywords
Commercial liquid pig manure; Esherichia coli 0157:H7; PCR; Salmonella spp.; Total aerobic bacteria;
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Times Cited By KSCI : 2  (Citation Analysis)
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