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Effect of Different Fertilization Management Practices on Soil Microbial Activities and Community Structure in Volcanic Ash Citrus Orchard Soil  

Joa, Jae-Ho (National Institute of Horticultural & Herbal Science, RDA)
Han, Seung-Gap (National Institute of Horticultural & Herbal Science, RDA)
Won, Hang-Yeon (National Academy of Agricultural Science, RDA)
Lim, Han-Cheol (National Institute of Horticultural & Herbal Science, RDA)
Hyun, Hae-Nam (Major of Plant Resources and Environment, Jeju National University)
Suh, Jang-Sun (National Academy of Agricultural Science, RDA)
Publication Information
Korean Journal of Soil Science and Fertilizer / v.42, no.3, 2009 , pp. 222-229 More about this Journal
Abstract
This study was performed to evaluate effect of different fertilization management practices on soil microbial activities and community structure using soil enzyme activities and PLFA contents in volcanic ash citrus orchard soil. Six experiment plots have differently managed based on the recommended application rate(NPK) of chemical fertilizer and compost for 13 years. Experiment plots were composed of no-fertilization(control), compost only, half amount of NPK with compost (1/2NPK+COM), NPK, NPK with compost(NPK+COM), and 3 times amount of NPK(3NPK). Soil samples collected in early March, May, July, and September 2007. Urease activity was high at NPK+COM in March, May, and September. It was higher in NPK+COM than in NPK. Urease activity decreased according to the order NPK>compost>control in March and May; compost>NPK>control in July and September. Dehydrogenase activity was significantly higher in 1/2NPK+COM($4.3ug\;TPF\;g^{-1}\;24h^{-1}$) than in control($2.4ug\;TPF\;g^{-1}\;24h^{-1}$), May. $\beta$-glucosidase activity was significantly higher in NPK and 1/2NPK+COM than in control, May. In March, Total PLFA contents were higher in NPK+COM($349.2n\;mol\;g^{-1}$) than in 3NPK($228.5n\;mol\;g^{-1}$). And that were higher in 1/2NPK+COM($237.8n\;mol\;g^{-1}$) than in 3NPK($133.1n\;mol\;g^{-1}$), May. Distribution ratio of soil microbial groups by PLFA biomaker were not significantly difference in between seasonal and treatments. Principal component analysis by PLFA profiles showed that microbial community in compost and 3NPK plot were different compared with other treatments in March. But Differences in compost and 3NPK plot were not found in May. Our result showed that the change of microbial community structure affected by fertilization effect and seasonable variation.
Keywords
Volcanic ash soil; Citrus; PLFA; Urease; Microbial community;
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