Browse > Article

Comparison of Biological Characteristics on the Organic Waste-treated Lysimeter Soil by RFLP, PLFA, and CLSU  

Jang, Kab-Yeul (Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA)
Weon, Hang-Yeon (Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA)
Lee, Kang-Hyo (Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA)
Kwon, Sun-Ik (Climate Change & Agroecology Division, National Academy of Agricultural Science, RDA)
Kong, Won-sik (Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA)
Suh, Jang-sun (Organic Agriculture Division, National Academy of Agricultural Science, RDA)
Sung, Jae-Mo (Department of Environmental Biology, Kangwon National University)
Publication Information
Korean Journal of Soil Science and Fertilizer / v.41, no.6, 2008 , pp. 415-418 More about this Journal
Abstract
The application of sludge wastes into agricultural fields has been increasing annually in Korea. In particular, sewage sludge application has been widely accepted in decades. Sewage sludge application aid in the recycling of essential nutrients and act as a source of organic matter improving the structure and water-holding properties of the soil. The efficient use of sludge wastes, however, requires an individual assessment of waste products. This study assessed the biological characteristics of organic waste-treated lysimeter soils and develop its indicator to assess the soil health of organic waste-treated lysimeter soils. Several analytical techniques more recently developed such as restriction fragment length polymorphism (RFLP), phospholipid fatty acid (PLFA), and community level substrate utilization (CLSU) fingerprints allow for detailed analyses of soil microbial communities. PLFA and RFLP was, therefore, used in the study to characterize the microbial communities in soil without the need to isolate individual fungi and bacteria. PLFA, RFLP and CLSU have been utilized to assess microbial characteristics of the lysimeter soils with four different sludge wastes for eight consecutive years. Each of these methods was analyzed for a different aspect of soil microbial characteristics. The study would disclose those methods yielded highly reproductive results for each soil and allow distinguishing the soils based on the structures of specific geneand PLFA-pools more than CLSU fingerprints. PLFA methods, especially, revealed the same relative similarities of the treated soils based on cluster analysis of the biological characteristics. Pig manure compost-treated soil, however, was only the same relative resemblance among the three methods. These results indicated that PLFA easily assessed the biological soil characterization.
Keywords
RFLP; PLFA; CLSU; Organic waste-treated soil; Bacteria;
Citations & Related Records
연도 인용수 순위
  • Reference
1 원항연, 권순우, 송재경, 남재작, 장갑열. 2004. 폐기물 연용지의 세균 분포조사. 농촌진흥청 농업과학기술원 농업환경연구.
2 원항연, 권장식, 서장선, 최우영. 1999. 돈분퇴비의 시용이 배추재배지 토양의 미생물상 및 화학성에 미치는 영향. 한국토양비료학회지. 32:76-83.
3 Felske, A., Wolterink, A., Lis, R.V., and Akkermans, A.D.L.. 1998. Phylogeny of the main bacterial 16S rRNA sequences in drentse a grassland soils (The Netherlands). Appl. Envir. Microbiol. 64(3):871-879.
4 Zelles, L., Bai, Q.Y., Beck, T., and Beese, F., 1992. Signature fatty acids in phospholipids and lipopolysaccharides as indicators of microbial biomass and community structure in agricultural soils. Soil Biology & Biochemistry 24: 317-323.   DOI   ScienceOn
5 Klamer, K., and Baath, E. 1998. Microbial community dynamics during composting of straw matetial studied using phospholipid fatty acid analysis. FEMS Microbiology Ecology 27 : 9-20.   DOI
6 Muyzer, G., and Smalla, K. 1998. Application of Denaturing Gradient Gel Electrophoresis (DGGE) and temprature gradient gel electrophoresis(TGGE) in microbial ecology.
7 Smalla, K. Wachtendorf, U., Heuer, H., LIU, W.T., and Forney, L. 1998. Analysis of BIOLOG GN substrate utilization patterns by microbial communities. Appl. Envir. Microbiol. 64(4):1220-1225.
8 Montagne, M.G.. Michel, F.C. Holden, P.A. and Reddy, C.A. 2002. Evaluation of extraction and purification methods for obtaining PCR-amplifiable DNA from compost for microbial community analysis. Journal of Microbiological Methods. 49: 255-264.   DOI   ScienceOn
9 Watanabe, K., 2001. Microorganisms relevant to bioremediation. Current Opinion in Biotechnology, 12(3): 237-241.   DOI   ScienceOn
10 Widmer, F., Seidler, R.J., Gillevet, P.M., Watrud, L.S., and Di Giovanni, G.D., 1998. A highly selective PCR protocol for detecting 16S rRNA genes of the genus Pseudomonas (sensu stricto) in environmental samples. Applied and Environmental Microbiology 64: 2545-553.
11 Pace, N.R., 1996. New perspective on the natural microbial world : molecular microbial ecology. ASM News 62 :463-470.
12 Widmer, F., Seidler, R.J., Gillevet, P.M., and Watrud, L.S., 2002. A highly selective PCR protocol for detecting 16S rRNA genes of the genus Pseudomonas (sensu stricto) in environmental samples. Appl. Envir. Microbiol. 1998 64: 2545-2553
13 권순익, 2003. 농촌진흥청 농업과학기술원 농업환경연구.
14 송인근, 김유영, 조흥범, 최영길. 1999. 토양세균 군집의 대사 다양성과 16S rDNA의 제한효소 지문분석에 의한 유전적 다양성의 비교. 한국미생물학회. 35(1): 72-77.
15 Zhou, G. Whong, W.Z. Ong, T. and Chen, B. 2000. Development of a fungus-specific PCR assay for detecting low-level fungi in an indoor environment. Molecular and Cellular Probes. 14(6): 339-348.   DOI   ScienceOn
16 Widmer, F., Fliebach, A., Laczko, E., Schulze-Aurich, J., and Zeyer, J. 2001. Assessing soil biological characteristics: a comparison of bulk soil community DNA-, PLFA-, and Biolog-analyses. Soil Biology & Biochemistry 33 : 1029-1036.   DOI   ScienceOn