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In vitro Evaluation of the Mechanism of Antagonism and Phosphate Solubilization by the Insect Gut Bacteria Pseudomonas sp. PRGB06 that Exhibits Plant Growth Promotion and Bio-Fertilizing Traits  

Kim, Kyoung-A (Department of Agricultural Chemistry, Chungbuk National University)
Indiragandhi, P. (Department of Agricultural Chemistry, Chungbuk National University)
Anandham, R. (Department of Agricultural Chemistry, Chungbuk National University)
Palaniappan, P. (Department of Agricultural Chemistry, Chungbuk National University)
Trivedi, P. (Department of Agricultural Chemistry, Chungbuk National University)
Madhaiyan, M. (Department of Agricultural Chemistry, Chungbuk National University)
Han, Gwang-Hyun (Department of Agricultural Chemistry, Chungbuk National University)
Sa, Tong-Min (Department of Agricultural Chemistry, Chungbuk National University)
Publication Information
Korean Journal of Soil Science and Fertilizer / v.41, no.1, 2008 , pp. 18-25 More about this Journal
Abstract
Pseudomonas sp. PRGB06, a bacterial strain isolated from diamondback moth (Plutella xylostella) gut, was examined for its plant growth promotion and biofertilizing traits. The bacteria growth was observed under various conditions of carbon sources, temperature, pH and salt concentrations. In addition, the mechanisms of antagonism and phosphate solubilization were investigated. The bacterial strain PRGB06, grew well using most of the tested carbon sources. The best growth was observed at $30^{\circ}C$ and pH 7. The inhibition of the pathogenic fungi was likely due to the volatile antifungal metabolite and ammonia gas produced by the bacteria. A significant positive relationship was found between the phosphate solubilization and acid production. When inoculated with PRGB06 in vitro and in gnotobiotic condition, red pepper and maize showed increase in root length, seedling vigor and dry bio-mass.
Keywords
Diamondback moth; Pseudomonas; Volatile antifungal metabolite; Ammonia; P solubilization; Red pepper; Maize;
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