Browse > Article

Inorganic Phosphate Solubilization by Immobilized Pantoea agglomerans under in vitro Conditions  

Kim, Eun-Hee (Department of Agricultural Chemistry, Chungbuk National University)
Park, Sung-Ae (Department of Agricultural Chemistry, Chungbuk National University)
Park, Myoung-Su (Department of Agricultural Chemistry, Chungbuk National University)
Yang, Jin-chul (Department of Agricultural Chemistry, Chungbuk National University)
Madhaiyan, Munusamy (Department of Agricultural Chemistry, Chungbuk National University)
Seshadri, Sundaram (Department of Agricultural Chemistry, Chungbuk National University)
Sa, Tong-Min (Department of Agricultural Chemistry, Chungbuk National University)
Publication Information
Korean Journal of Soil Science and Fertilizer / v.37, no.1, 2004 , pp. 36-40 More about this Journal
Abstract
It is now widely accepted that immobilized microbial cells can overcome some of the problems associated with microbial survival stability, efficacy, storage, transportation and ease of application in agricultural environments. Pantoea agglomerans, a phosphate solubilizing bacterium, was immobilized in alginate, agar and gelatin carriers. All the three immobilfized carriers with bacterial cells of P. agglomerans were compared for solubilization of tricalcium phosphate in pure liquid cultures. While alginate beads were tested for phosphate solubilization on alternate days up to five days, agar beads and gelatin cubes were subjected for one time phosphate solubilization analysis after seven days. Both alginate and agar immobilized cells of P. agglomerans exhibited higher efficiency in increasing the solubilizaliun of tricalcium phosphate than gelatin immobilized cells. The culture filtrate of alginate bead inoculation treatment registered a rapid increase in soluble phosphate concentration upon incubation. A corresponding decrease in the pH of the medium was also observed in all the treatments.
Keywords
Immobilization; Pantoea agglomerans; Phosphate solubilization; Tricalcium phosphate;
Citations & Related Records
연도 인용수 순위
  • Reference
1 de Alteriis, E,. P. Parascandola, S. Salvadore, and V. Sardi. 1985. Enzymc immobilization within insolubilized gelatin. J. Chem. Technol. Biot. 35(B):60   DOI
2 Ehrlich, H. L. 1990. Mikrobiologische und biochemische Verfahren stechnik, In A. Einsele et al. (ed.) Geomicrobiology, 2nd ed. VCH Verlagsgesellschafat, Weinheim, Germany
3 Hinsinger, P. 2001. Bioavailability of soil inorganic P in the rhizosphere as affected by root induced chemical changes: a review. Plant Soil 237:173-195   DOI   ScienceOn
4 Lopez, A., N. Lazaro, and A. M. Marques. 1997. The interphase technique: a simple method of cell immobilization in gel beads. J. Microbiol. Meth. 30:231-234   DOI   ScienceOn
5 Pikovskaya, R. I. 1948. Mobilization of phosphorus in soil in connection with vital activity of some microbial species. Microbiologiya 17:362-370
6 Vassileva, M., R. Azcon, J. M. Barea, and N. Vassilev. 1998. Application of an encapsulated filamentous fungus in solubilization of inorganic phosphate. J. Biotechnol. 63:67-72   DOI   ScienceOn
7 Vassilev, N., M. Vassileva, R. Azcon, and J. M. Barea. 2001. Interactions of an arbuscular mycorrhizal fungus with free or coencapsulated cells of Rhizobium trifoli and Yarowia lipotytica inoculated into a soil plant system. Biotechnol. Lett. 23:149-151   DOI   ScienceOn
8 Woodward, J. 1988. Methods of immobilization of microbial cells. J. Miciobiol. Meth. 8:91-102   DOI   ScienceOn
9 Vassileva, M., R. Azcon, J. M. Barea, and N. Vassilev. 2000. Rock phosphate solubilization by free and encapsulated cells of Yarowia lipolytica. Process Biochem. 35:693-697   DOI   ScienceOn
10 Vassilev, N., M. Toro, M. Vassileva, R. Azcon, and J. M. Barea. 1997b. Rock phosphate solubilization by immobilized cells of Enterobacter sp. in fermentation and soil conditions. Bioresource Technol. 61:29-32   DOI   ScienceOn
11 Chung, H. K. 2003. Identification of phosphate solubilizing bacteria isolated from rhizosphere. M.S. Thesis, Chungbuk National University, Cheongju, Korea
12 Fedirici, F. 1993. Potential application of viable, immobilized fungal cell systems. World J. Microb. Biot. 9:495-502   DOI   ScienceOn
13 Goldstein, A. H., R. D. Rogers, and G. Mead. 1993. Separating phosphate from ores via bioprocessing. Nat. Biotechnol. 11:250-254   DOI
14 Van Elsas, J. D., and C. E. Heijnen. 1990. Methods for the introduction of bacteria into soil: a review. Biol. Fert. Soils 10:127-133   DOI
15 Zayed, G. 1997. Can immobilization of Bacillus megaterium cells in alginate beads protect them against bacteriophages. Plant Soil 197:1-7   DOI   ScienceOn
16 Rodrigues, H., and R. Fraga. 1999. Phosphate solubilizing bacteria and their role in plant growth promotion. Biotechnol. Adv. 17:319-339   DOI   ScienceOn
17 Bashan, Y., and L. E. Gonzalez. 1999. Long tern survival of the plant growth promoting bacteria Azospirillum brasilense and Pseudomonas fluorescence in dry alginate inoculant. Appl. Microbiol. Biot. 51:262-266   DOI   ScienceOn
18 Deelereck, S., D. G. Strullu, C. Plenchette, and T. Guillemette. 1996. Entrapment of in vitro produced spores of Glomus versiforme in alginate beads: in vitro and in vivo inoculum potentials. J. Biotechnol. 48:51-57   DOI   ScienceOn
19 Fenice, M., L. Selbman, F. Federici, and N. Vassilev. 2000. Application of encapsulated Penicillium variabile P16 in solubilization of rock phosphate. Bioresource Technol. 73:57-162
20 Murphy, J., and J. P. Riley. 1962. A modified single solution method for the determination of phosphate in natural waters. Anal. Chim. Acta. 27:31-36   DOI   ScienceOn
21 Vassilev, N., M. Vassileva, and R. Azcon. 1997a. Solubilization of rock phosphate by immobilized Aspergillus niger. Bioresource Technol. 59:1-4   DOI   ScienceOn
22 Bashan, Y. 1998. Inoculants of plant growth promoting bacteria for use in adriculture. Biotechnol. Adv. 16:729-770   DOI   ScienceOn