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http://dx.doi.org/10.5141/ecoenv.2014.004

Effect of N-acetyl-L-cysteine on human chronic myeloid leukemia cells KCL22 treated with mitomycin C  

Simonyan, Anna (Dept. of Genetics and Cytology, Faculty of Biology, Yerevan State University)
Hovhannisyan, Galina (Dept. of Genetics and Cytology, Faculty of Biology, Yerevan State University)
Aroutiounian, Rouben (Dept. of Genetics and Cytology, Faculty of Biology, Yerevan State University)
Kim, Jin Kyu (Korea Atomic Energy Research Institute, Advanced Radiation Technology Institute)
Publication Information
Journal of Ecology and Environment / v.37, no.1, 2014 , pp. 31-34 More about this Journal
Abstract
The effectiveness of N-acetyl-L-cysteine (NAC) to protect blood cells against Mitomycin C (MMC) induced genotoxicity was investigated in human chronic myeloid leukemia cells (KCL22) using the alkaline comet assay. The comet assay was selected as sensitive and rapid method for analysis of DNA damage and repair in individual cells. NAC treatment alone did not produce any damage in KCL22 cell. But NAC was found to be effective in reducing genotoxic damage in KCL22 cells exposed to MMC. These results confirm the literature data that, given the safety and ability to reduce DNA damage. NAC may be useful to prevent drug-mediated genotoxicity.
Keywords
comet assay; genotoxicity prevention; mitomycin C; N-acetyl-L-cysteine;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
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