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http://dx.doi.org/10.5141/JEFB.2006.29.1.061

Changes of Thiols and Oxidative Stress in Tomato Seedlings Exposed to Cadmium  

Cho, Un-Haing (Department of Biology, Changwon National University)
Seo, Nam-Ho (Department of Biology, Changwon National University)
Publication Information
Journal of Ecology and Environment / v.29, no.1, 2006 , pp. 61-67 More about this Journal
Abstract
Tomato (Lycopersicon esculentum Mill) seedlings exposed to various concentrations of $CdCl_2(0{\sim}100{\mu}M)$ in a nutrient solution for up to 9 days were analyzed with respect to the thiol changes and oxidative stress. The Cd exposure increased total non-protein thiols (NPT) and cysteine in both leaves and roots, total glutathione in leaves, and the ratios of oxidized glutathione (GSSG)/reduced glutathione (GSH) in both leaves and roots, but decreased the ratio of dehydroascorbate (DASA)/ascorbate(ASA) in leaves. Our results suggest that the Cd-induced GSH depletion due to thiol synthesis and oxidation alters the antioxidant activity of seedlings for $H_2O_2$, and the subsequent $H_2O_2$ accumulationand oxidative stress result in phytotoxicity.
Keywords
Cadmium; Oxidative stress; Thiols; Tomato;
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