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http://dx.doi.org/10.7316/KHNES.2016.27.4.378

Performance Improvement of Glucose Sensor Adopting Enzymatic Catalyst bonded by Glutaraldehyde  

AHN, YEONJOO (Grad. School of Energy and Environment, Seoul National Univ. of Science and Technology)
CHUNG, YONGJIN (Grad. School of Energy and Environment, Seoul National Univ. of Science and Technology)
LEE, KYUBIN (Grad. School of Energy and Environment, Seoul National Univ. of Science and Technology)
KWON, YONGCHAI (Grad. School of Energy and Environment, Seoul National Univ. of Science and Technology)
Publication Information
Transactions of the Korean hydrogen and new energy society / v.27, no.4, 2016 , pp. 378-385 More about this Journal
Abstract
In this study, we synthesized a biocatalyst consisting of glucose oxidase (GOx), polyethyleneimine (PEI) and carbon nanotube (CNT) with addition of glutaraldehyde (GA)(GA/[GOx/PEI/CNT])for fabrication of glucose sensor. Main bonding of the GA/[GOx/PEI/CNT] catalyst was formed by crosslinking of functional end groups between GOx/PEI and GA. Catalytic activity of GA/[GOx/PEI/CNT] was quantified by UV-Vis and electrochemical measurements. As a result of that, high immobilization ratio of 199% than other catalyst (with only physical adsorption) and large sensitivity value of $13.4{\mu}A/cm^2/mM$ was gained. With estimation of the biosensor stability, it was found that the GA/[GOx/PEI/CNT] kept about 88% of its initial activity even after three weeks. It shows GA minimized the loss of GOx and improved sensing ability and stability compared with that using other biocatalysts.
Keywords
Glucose; Glucose oxidase; Protein immobilization; Biosensor; Amperometry;
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