Browse > Article

Optimization of Hydrogen Production Using the Mixed Microflora Isolated from Sewage Sludge  

Oh, You-Kwan (Bioenergy Research Center, Korea Institute of Energy Research)
Kim, Mi-Sun (Bioenergy Research Center, Korea Institute of Energy Research)
Publication Information
Transactions of the Korean hydrogen and new energy society / v.19, no.1, 2008 , pp. 41-48 More about this Journal
Abstract
Fermentative $H_2$ production was studied using microbial consortia isolated from heat-treated ($90{\circ}C$, 20 min) sewage sludge. Important parameters investigated were carbon(C) and nitrogen(N)-sources, C/N ratio, phosphate concentration, pH and temperature during anaerobic cultivation in serum bottles. Starch, ribose, sucrose and glucose were good C-sources for the culture growth and $H_2$ production. Yeast extract was better N-source than $(NH_4)_2SO_4$ or peptone when individually added to the synthetic media, however the combination of above three N-sources exhibited the additional effect for cell growth and $H_2$ evolution. Addition of 100 mM phosphate as a buffering agent prevented the rapid pH drop during the cultivation. The optimum initial pH for the cell growth was at 7.0, whereas $H_2$ production was observed at pH 5.5. Optimum temperature for the cell growth and $H_2$ production was $37{\circ}C$. Initial C/N ratio of 1.22 in the media using glucose and yeast extract as the C- and N-sources, respectively, showed the $H_2$ yield 1.0 mol $H_2$/mol glucose.
Keywords
hydrogen production; anaerobic fermentative bacteria; optimization; carbon/nitrogen ratio; hydrogen production yield;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
연도 인용수 순위
1 C. Collet, N. Adler, J. P. Schwitzguebel, and P. Peringer, 'Hydrogen production by Clostridium thermolacticum during continuous germentation of lactose', Int. J. Hydrogen Energy, Vol 29, 2004, pp. 1479-1485   DOI   ScienceOn
2 D. Das and T. N. Veziroglu, 'Hydrogen production by biological processes', Int J. Hydrogen Energy, Vol. 26, 2001, pp. 13-28   DOI   ScienceOn
3 H. P. P Herber and H. Liu, 'Effect of pH on hydrogen production from glucose by a mixed culture', Bioresource Technol., Vol. 82, 2002, pp. 87-93   DOI   ScienceOn
4 S.-E. Oh, P. Iyer, M. A. Bruns, and B. E. Logan, 'Biological hydrogen production using a membrane bioreactor', Biotechnol. Bioeng., Vol. 87, 2004, pp. 119-127   DOI   ScienceOn
5 M. Heyndrickx, P. De Vos, and J. De Ley, 'Fermentation characteristics of Clostridium pasteurianum LMG 3285 grown on glucose and mannitol', J. Appl. Bacteriol. Vol. 70, 1991, pp. 52-58   DOI
6 L. J. Stal and R. Moezelaar, 'Fermentation in cyanobacteria', FEMS Microbiol. Rev., Vol. 21, 1997, pp. 179-211   DOI   ScienceOn
7 I. -S. Chang, P. Le Clech, B. Jefferson, and S. Judd, 'Membrane fouling in membrane bioreactors for wastewater treatment', J. Envir. Engrg, Vol. 128, 2002, pp. 1018-1029   DOI   ScienceOn
8 F. R. Hawkes, R. Dinsdale, D. L. Hawkes, and I. Hussy, 'Sustainable fermentative hydrogen production, challenges for process optimisation', Int. J. Hydrogen energy, Vol. 27, 2002, pp. 1339-1347   DOI   ScienceOn
9 C. Y. Lin and C. H. Lay, 'Carbon/nitrogenratio effect on fermentative hydrogen production by mixed microflora', Int. J. Hydrogen Energy, Vol. 29, 2004, pp. 41-45   DOI   ScienceOn
10 B. K. Ahring, K. Jensen, P. Nielsen, A. B. Bjerre, and A. S. Schmid, 'Pretreatment of wheat straw and conversion of xylose and xylan to ethanol by thermophilic anaerobic bacteria', Bioresource Technol, Vol. 58, 1996, pp. 107-113   DOI   ScienceOn
11 O. Mizno, R. Dinsdale, F. R. Hawkes, D. L. Hawkes, and T. Noike, 'Enhancement of hydrogen production from glucose by nitrogen gas sparging', Bioresource Technol., Vol. 73, 2000, pp. 59-65   DOI   ScienceOn
12 J.-H. Shin and T. H. Park, 'Biological hydrogen production process', Korean Chem. Eng. Res., Vol. 44, 2006, pp. 16-22
13 H. Koku, I. Erolu, U. Gunduz, M. Yucel, and L. Turker, 'Aspects of the metabolism of hydrogen production by Rhodobacter sphaeroides', Int. J. Hydrogen Energy, Vol. 27, 2002, pp. 1315-1329   DOI   ScienceOn
14 K. S. Lee, C. M. Kang, and S. Y. Chung, 'Medium composition of Enterobacter cloacae YJ-1 for maximizing hydrogen production', Korean J. Biotechnol. Bioeng., Vol. 20, 2005, pp. 350-354   과학기술학회마을
15 E. W. J. van Niel, P. A. M. Claassen, and A. J. M. Stams, 'Substrate and product inhibition of hydrogen production by the extreme thermophile, Caldicellulosiruptor saccharolyticus', Biotechnol. Bioeng., Vol. 81, pp. 255-262   DOI   ScienceOn
16 C. Y. Lin and R. C. Chang, 'Hydrogen production during the anaerobic acidogenic conversion of glucose', J. Chem. Technol. Biotechnol., Vol. 74, 1999, pp. 498-500   DOI   ScienceOn