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Influence of light intensity and photo-bioreactor design for photo biological hydrogen production by Rhodobactor sphaeroides  

Kim, Mi-Sun (Biomass research center, Korea Institute of Energy Research)
Baek, Jin-Sook (Biomass research center, Korea Institute of Energy Research)
Publication Information
Transactions of the Korean hydrogen and new energy society / v.15, no.2, 2004 , pp. 166-174 More about this Journal
Abstract
Purple non-sulfur bacteria, Rhodobacter sphaeroides KD131 grew to reach the maximum cell concentration in 45 hrs of incubation in the synthetic media containing (NH4)2SO4, L-aspartic acid and succinic acid as the carbon and nitrogen sources, respectively, at 30oC under 8 klux irradiance using halogen lamp. The strain produced hydrogen from the middle of the logarithmic growth phase and continued until the cell growth leveled out. The strain grew and produced hydrogen under the irradiance of 3-30 klux, but cell growth was inhibited over 100 klux. In addition, anaerobic/light culture condition was better than the aerobic/dark on the hydrogen production. Among various photo-bioreactors examined, the flat-vertical reactor manufactured using clear acrylic plastic material showed the best hydrogen production rate at the given culture condition.
Keywords
Photosynthetic bacteria; Rhodobacter sphaeroides KD131; hydrogen; photo-bioreactor; light intensity;
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