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http://dx.doi.org/10.13000/JFMSE.2014.26.6.1402

Morphological Observation of Pre- and Postovulated Eggs from Artificially Maturated Wild and Feminized Eels  

Lee, Nam-Sil (New Strategy Research Center, NFRDI)
Kim, Shin-Kwon (New Strategy Research Center, NFRDI)
Lee, Bae-Ik (New Strategy Research Center, NFRDI)
Kim, Dae-Jung (New Strategy Research Center, NFRDI)
Publication Information
Journal of Fisheries and Marine Sciences Education / v.26, no.6, 2014 , pp. 1402-1411 More about this Journal
Abstract
This study about morphological observation of pre- or post ovulated eggs obtained from artificially maturated female eels. Female eels were divided with two groups as wild eels from nature and feminized eels from farm. Artificial maturation had been conducted with the established methods in this laboratory, and then maturate eggs sampled at fixed 3 times and these were observed with stereomicroscope. Rate of increased body weight (RIW) were measured with 2 times. Egg diameters and development of oil droplets are determined for standardization of egg maturation degree, and the transparency of egg cytoplasm and the homogeneous degrees of egg size were referred to determine of egg quality. Rate of increased body weight (RIW) were good in range about 10 % at final salmon pituitary extracts (SPE) injection time and in range about 20 % at $17{\alpha}$, $20{\beta}$-dihydroxy-4-pregnen-3-one (DHP) injection time. Good matured egg for fertilization had $900-1000{\mu}m$ diameter, and they had about 50 oil droplets in size about $100{\mu}m$ diameter. There were not predominant differences at egg size and oil droplet development between wild female and feminized eels.
Keywords
Artificial production of eel; Feminized eel; Egg quality; Morphology; Wild female eel;
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Times Cited By KSCI : 5  (Citation Analysis)
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1 Pedersen Benedikte Hedegaard (2003) Induced sexual maturation of the European eel Anguilla anguilla and fertilisation of the eggs, Aquaculture 224, 323-338.   DOI   ScienceOn
2 Kim, Dae-Jung et al. (2013) Effect of Estradiol-$17{\beta}$ on the feminization of Japanese Eel, Anguilla japonica, Journal of Life Science 23, 998-1003.   DOI   ScienceOn
3 Ohta, Hiromi et al. (1996) Changes in fertilization and hatching rates with time after ovulation induced by $17{\alpha},20{\beta}$-dihydroxy-4-pregnen-3-one in the Japanese eel, Anguilla japonica, Aquaculture 139, 291-301.   DOI
4 Ohta, Hiromi et al. (1997) Artificial induction of maturation and fertilization in the Japanese eel, Anguilla japonica, Fish Physiology and Biochemistry 17, 163-169.   DOI   ScienceOn
5 Stottrup, Josianne et al. (2013) Modification of essential fatty acid composition in broodstock of cultured European eel Anguilla anguilla L. Aquaculture Nutrition, Aquaculture 19, 172-185.
6 Tanaka, Hideki.Kagawa, Hirohiko & Ohta, Hiromi (2001) Production of leptocephali of Japanese eel, Anguilla japonica in captivity, Aquaculture 201, 51-60.   DOI   ScienceOn
7 Tanaka, Hideki et al. (2003) The first production of glass eel in captivity: fish reproductive physiology facilitates great progress in aquaculture, Fish Physiology and Biochemistry 28, 493-497.   DOI   ScienceOn
8 Tachiki, Hiroyuki & Nakagawa, Takeyoshi (1992) Induction of spawning in female cultured eel Anguilla japonica, Bulletin Aichi Fisheries Research Institute. 1, 79-83.
9 Tomkiewicz, Jonna et al (2013) Reproduction of European eel and larval culture, Aquaculture europe 13, RoomR8.
10 Unuma, Tatuya et al. (2004) Determination of the rates of fertilization, hatching and larval survival in the Japanese eel, Anguilla japonica, using tissue culture microplates. Aquaculture, 241, 345-356   DOI
11 Yamamoto, Kiichiro & Yamauchi, Kohei (1974) Sexual maturation of Japanese eel and production of eel larvae in the aquarium, Nature, 251, 220-222   DOI   ScienceOn
12 Yoshikawa, Masayuki (2012) Improvement in hatching rates in the Japanese eel Anguilla japonica by the control of rearing temperatures in the late stage of maturation in the female parents, Aquaculture, 338-341, 223-227   DOI
13 Kagawa, Hirohiko et al. (1995). In vitro Effects of $17{\alpha}$-hydroxyprogesterone and $17{\alpha},20{\beta}$-dihydorxy-4-pregnen-3-one on final maturation of oocytes at various developmental stages in artificially matured Japanese eel Anguilla japonica, Fisheries Science 61, 1012-1015.
14 Fernandez-Palacios et al. (1995) Effect of n-3 HUFA level in broodstock diets on egg quality of gilthead seabream (Sparus aurata L.) Aquaculture 132, 325-337.   DOI
15 Cerda, Joan et al.(1994) Influence of nutritional composition of diet on sea bass, Dicentrarchus labrax L., reproductive performance and egg and larval quality, Aquaculture 128, 345-361.   DOI
16 Ciereszco, Andrzej & Dabrowski, Konrad(1995) Sperm quality and ascorbic acid concentration in rainbow trout semen are affected by dietary vitamin C: an across season study, Biology of Reproduction. 52, 982-988.   DOI   ScienceOn
17 Constantinois C Mylonas & Yonathan Zohar (2001) Endocrine regulation and artificial induction of oocyte maturation and spermiation in basses of the genus Morone, Aquaculture 202, 205-220.   DOI
18 Izquierdo M.S. Fernandez-Palacioa H. & Tacon A.G.J. (2001). Effect of broodstock nutrition on reproductive performance of fish, Aquaculture 197, 25-42.   DOI
19 Kagawa, Hirohiko et al. (1997) Induced ovulation by injection of $17{\alpha},20{\beta}$-dihydorxy-4-pregnen-3-one in the artificially matured Japanese eel, with special reference to ovulation time, Fisheries Science 63, 365-367.
20 Kim, Dae-Jung et al. (2006a). Plasma sex steroid hormone profiles in artificially maturing wild eel, Anguilla japonica, Journal of Aquaculture 19, 267-274.   과학기술학회마을
21 Kim, Eung-Oh et al. (2006b) Plasma sex steroid hormone profiles and testicular development in artificially maturing cultured male eel, Anguilla japonica, Journal of Korean Fisheries Society 39, 466-471.
22 Kim, Dae-Jung.Bae, Jun Young & Kim, Eung-Oh (2007a) Changes in sex steroid hormones and ovarian development during artificial maturation of female eel, Anguilla japonica, Integrative Biosciences 11, 117-124.   과학기술학회마을
23 Kim, Dae-Jung et al. (2007b) Development of the eggs and pre-leptocephalus larvae by natural spawning of artificially-matured Japanase eel, Anguilla japonica, Journal of Aquaculture 20, 160-167.