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http://dx.doi.org/10.13000/JFMSE.2013.25.6.1360

Survival and Histological Change of Integumentary System of the Juvenile Sea Cucumber, Apostichopus japonicus Exposed to Various Salinity Concentrations  

Kim, Tae-Ik (Southwest Sea Fisheries Research Institute, NFRDI)
Park, Min-Woo (Southwest Sea Fisheries Research Institute, NFRDI)
Cho, Jae-Kwon (Southwest Sea Fisheries Research Institute, NFRDI)
Son, Maeng-Hyun (Southwest Sea Fisheries Research Institute, NFRDI)
Jin, Young-Guk (Southwest Sea Fisheries Research Institute, NFRDI)
Publication Information
Journal of Fisheries and Marine Sciences Education / v.25, no.6, 2013 , pp. 1360-1365 More about this Journal
Abstract
Juvenile sea cucumber, Apostichopus japonicus(wet weight $1.0{\pm}0.2g$, n=250) acclimated to environmental conditions($17^{\circ}C$, 33 psu) during 4 weeks for observation of survival and structural variation of integumentary system according to various salinity concentration. After that, it was exposed 0, 5, 10, 15, 20, 25, 30, 35, 40 psu in 96h, and recovered during 7 days. In this results, The 96-h lethal concentration(LC50) for sea cucumber was a salinity of 21.05 psu. Integumentary system of the sea cucumber exposed to below the salinity of 20 psu mainly observed thickness reduction of epidermal layer, nucleus condensation of epithelial cells, decreased of mucous cells and loose arrangement of connective tissue in dermal layer. Integumentary system of the sea cucumber exposed a salinity of 40 psu mainly observed nucleus hypertrophy of epithelial cells, increase of mucous cells and tight arrangement of connective tissue in dermal layer. Also, observed secretory cells showing the alcian blue(pH 2.5) positive. During 96-h exposure, this cells decreased after increase below the salinity of 20 psu but increased in the salinity of 40 psu compared with 25-35 psu.
Keywords
Apostichopus japonicus; salinity; survival; integumentary system;
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