Production of Antithrombotic Material Extracted from Auricularia auricular-judae and the Verification of Its Antithrombotic Activity via Animal Test

Large-scale preparation steps of antithrombotic materials from wood ear mushroom (Auricularia auricular-judae) were established as follows. Grounded dry wood ear mushroom was extracted with 75% ethanol and its precipitate was extracted with $76^{\circ}C$ water for 2 hr followed by filter pressing. The filtrate was then concentrated by vacuum and extracted with 80% ethanol, and the resulting precipitate was then freeze-dried. The formula of the product was determined using consumer susceptibility tests as follows; mushroom extract 90.5%, high fructose corn syrup 2.0%, $\beta$-cyclodextrin 1.5%, fructo-oligosaccharide 2.0%, pear puree 4.0%. When the packed products were stored at 25, 37, or $45^{\circ}C$ for 8 weeks, there were no noticeable changes in water activity, moisture content, pH, and acidity. The viable cell number of total bacteria was slightly increased during the storage period at 25 and $37^{\circ}C$, The total bacteria were not detected in the product when stored at $45^{\circ}C$. When the product was injected intravenously into rat at the level of 1,000 mg/kg, antithrombotic activities such as activated partial thromboplastin time, thrombin time, prothrombin time, and FIB were increased when compared with the control group. When the product was administrated orally into rat at the level of 500 mg/kg, it showed the same antiplatelet activity to aspirin.

목이버섯으로부터 추출한 항혈전물질의 제품화와 동물실험을 통한 항혈전활성 검증

목이버섯으로부터 항혈전물질을 추출하여 제품화하기 위한 대량생산공정을 다음과 같이 확립하였다. 즉, 건조된 목이버섯을 분쇄하여 75% ethanol로 추출하여 원심분리한 침전물에 증류수에 첨가한 후 $76^{\circ}C$에서 2시간 추출한 후 압착여과하여 여과액을 얻는다. 이를 감압농축한 후 여기에 최종 농도가 80%가 되도록 ethanol을 첨가하여 추출한 침전물을 동결건조한다. 제품의 소비지 기호도 조사를 통해 부재료의 종류와 첨가량을 결정하여 배합비를 목이버섯 추출물 90.5%, 고과당 2.0%, $\beta$-cyclodextrin 1.5%, 올리고 당 2.0%, 배 농축액 4.0%로 결정하였다. 제품을 포장하여 25, 37, $45^{\circ}C$에서 저장하였을 경우 저장 8주에서도 수분활성도, 수분함량, pH, 산도에 큰 변화가 없었다. 미생물 생균수는 $25^{\circ}C$와 $37^{\circ}C$에서 저장하였을 경우 저장기간이 지남에 따라 약간 증가한 반면, $45^{\circ}C$에서 저장하였을 경우에는 저장 2개월 후에는 대부분 사멸하였다. 제품을 쥐에 정맥투여하였을 경우 1,000 mg/kg 투여 시 APTT 활성, TT 활성, PT 활성, FIB 활성이 대조구와 비교하여 유의적으로 높았으며 500 mg/kg을 경구투여하였을 경우 항혈소판활성이 아스피린과 동일한 수준을 나타내었다.