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Exploration of β-Glucosidase Activity of Lactic Acid Bacteria Isolated from Kimchi  

Jang, Mi-Hee (School of Biotechnology and Bioengineering, Kangwon National University)
Kim, Myoung-Dong (School of Biotechnology and Bioengineering, Kangwon National University)
Publication Information
Food Engineering Progress / v.14, no.3, 2010 , pp. 243-248 More about this Journal
Abstract
The ${\beta}$-glucosidase (E.C. 3.2.1.21) production capabilities of lactic acid bacteria isolated from a variety of kimchi (fermented vegetables) were examined. When grown in a medium containing cellobiose as carbon source, most lactic acid bacteria showed significantly higher intracellular levels of ${\beta}$-glucosidase than the extracellular levels. A maximum intracellular ${\beta}$-glucosidase activity of 3.7${\pm}$0.5 (unit/mg protein) was obtained in the case of Weissella cibaria KFRI88010 isolated from kimchi. The optimum reaction conditions for W. cibaria KFRI88010 ${\beta}$-glucosidase activity were pH 5.0 and ${37^{\circ}C}$, and addition of divalent cations to the reaction mixture resulted in a notable decrease in enzyme activity. The ${\beta}$-glucosidase activity was enhanced twofold when W. cibaria KFRI88010 was grown in a medium containing fructose as compared with to a medium containing glucose or cellobiose.
Keywords
${\beta}$-glucosidase; kimchi; lactic acid bacteria; Weissella cibaria;
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Times Cited By KSCI : 5  (Citation Analysis)
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