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Exploration of β-Glucosidase Activity of Lactic Acid Bacteria Isolated from Kimchi  

Jang, Mi-Hee (School of Biotechnology and Bioengineering, Kangwon National University)
Kim, Myoung-Dong (School of Biotechnology and Bioengineering, Kangwon National University)
Publication Information
Food Engineering Progress / v.14, no.3, 2010 , pp. 243-248 More about this Journal
Abstract
The ${\beta}$-glucosidase (E.C. 3.2.1.21) production capabilities of lactic acid bacteria isolated from a variety of kimchi (fermented vegetables) were examined. When grown in a medium containing cellobiose as carbon source, most lactic acid bacteria showed significantly higher intracellular levels of ${\beta}$-glucosidase than the extracellular levels. A maximum intracellular ${\beta}$-glucosidase activity of 3.7${\pm}$0.5 (unit/mg protein) was obtained in the case of Weissella cibaria KFRI88010 isolated from kimchi. The optimum reaction conditions for W. cibaria KFRI88010 ${\beta}$-glucosidase activity were pH 5.0 and ${37^{\circ}C}$, and addition of divalent cations to the reaction mixture resulted in a notable decrease in enzyme activity. The ${\beta}$-glucosidase activity was enhanced twofold when W. cibaria KFRI88010 was grown in a medium containing fructose as compared with to a medium containing glucose or cellobiose.
Keywords
${\beta}$-glucosidase; kimchi; lactic acid bacteria; Weissella cibaria;
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Times Cited By KSCI : 5  (Citation Analysis)
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1 Bae SS, Ahn C. 1997. Antibiosis and bacteriocin production of lactic acid bacteria isolated from Kimchi. J. Food Sci. Nutr. 2: 109-120.
2 Barras F, Chambost JP, Chippaux M. 1984. Cellobiose metabolism in Erwinia: genetic study. Mol. Gen. Genet. 197: 486-490.   DOI
3 Chun SB, Kim DH, Kim KH, Chung KC. 1991. Purification and characterization of ${\beta}$-glucosidase from Penicillium verruculosum. J. Microbiol. Biotechnol. 1: 188-196.
4 Estruch JJ, Chriqui D, Grossmann KL, Schell J, Spena A. 1991. The plant oncogene rolC is responsible for the release of cytokinins from glucoside conjugates. EMBO J. 10: 2889-2895.
5 Gunata YZ, Bayonove CL, Baumes RL, Cordonier RE. 1985. The aroma of grapes I. Extraction and determination of free and glycosidically bound fractions of some grape aroma components. J. Chromatogr. 331: 83-90.   DOI
6 Hong SW, You LK, Jung BM, Kim WS, Chung KS. 2009. Characterization of ${\alpha}$-galactosidase and ${\beta}$-glucosidase by Weissella cibaria. Korean J. Microbiol. Biotechnol. 37: 204-212.   과학기술학회마을
7 Jin HS, Kim JB, Yun YJ, Lee KJ. 2008. Selection of Kimchi starters based on the microbial composition of Kimchi and their effects. J. Korean Soc. Food Sci. Nutr. 37: 671-675.   과학기술학회마을   DOI
8 Joo AR, Jeya M, Lee KM, Sim WI, Kim JS, Kim IW, Kim YS, Oh DK, Gunasekaran P, Lee JK. 2009. Purification and characterization of a ${\beta}$-1,4-glucosidase from a newly isolated strain of Fomitopsis pinicola. Appl. Microbiol. Biotechnol. 83: 285-294.   DOI   ScienceOn
9 Juhasz T, Kozma K, Szengyel Z, Reczey K. 2003. Production of ${\beta}$-glucosidase in mixed culture of Aspergillus niger BKMF 1305 and Trichoderma reesei RUT C30. Food Technol. Biotechnol. 41:49-53.
10 Jun HK, Bae KM, Kim YH, Baik HS. 2000. Production and characterization of cyclodextrin glucanotransferase from Bacillus sp. JK-43 isolated from Kimchi. J. Korean Soc. Food Sci. Nutr. 29: 41-48.
11 Kawamori M, Takayama K, Takasawa S. 1987. Production of cellulases by a thermophilic fungus Thermoascus aurantiacus A-131. Agric. Biol. Chem. 51: 647-654.   DOI
12 Kim JH, Lee BR, Moon YP. 1998. Overproduction and secretion of ${\beta}$-glucosidase in Bacillus subtilis. J. Microbiol. Biotechnol. 8: 141-145.
13 Kim JY, Kim BK, Kang CS, Yi YS, Ahn JH, Lim YH. 2009. Cloning of ${\beta}$-glucosidase gene from Streptomyces coelicolor A3(2) and characterization of the recombinant ${\beta}$-glucosidase expressed in Escherichia coli. Korean J. Microbiol. Biotechnol. 37: 99-104.   과학기술학회마을
14 Kwark KS, Cu JG, Bae KM, Jun HK. 1999. Characterization of bacteriocin production by Lactococcus sp. J-105 isolated from Kimchi. Korean J. Life Sci. 9: 111-120.
15 Kim YW, Chun SS, Kim SJ, Chung YC, Sung NK. 1993. Cloning and expression of ${\beta}$-1,4-glucosidase gene from Pseudomonas sp. in Escherichia coli and Bacillus subtilis. Korean J. Appl. Microbiol. Biotechnol. 21: 113-118.
16 Ko JL, Oh CK, Oh MC, Kim SH. 2009. Isolation and identification of lactic acid bacteria from commercial Kimchi. J. Korean Soc. Food Sci. Nutr. 38: 732-741.   과학기술학회마을   DOI
17 Kurosawa K, Hosoguchi M, Hariantono J, Sasaki H, Takao S. 1989. Degradation of tough materials by cellulase from Corticium rolfsii. Agric. Biol. Chem. 53: 931-937.   DOI
18 Lee HS, Min KH, Bae M. 1998. Biosynthetic regulation and enzymatic properties of ${\beta}$-glucosidase from Cellulomonas sp. CS 1-1. Korean J. Appl. Microbiol. Bioeng. 2: 119-125.
19 Li X, Pei J, Wu G, Shao W. 2005. Expression, purification and characterization of a recombinant ${\beta}$-glucosidase from Volvariella volvacea. Biotech. Lett. 27: 1369-1373.   DOI   ScienceOn
20 Min EG, Han YH. 2000. Characteristics of extracellular ${\beta}$-glucosidase in Tricholoma matsutake. Korean J. Biotechnol. Bioeng. 15: 9-13.
21 Moon IS, Park SK, Lee KY. 1993. Production of ${\beta}$-glucosidase from Aspergillus niger. Korean J. Biotechnol. Bioeng. 8: 409-414.
22 Park KH, Oh TK, Shin JD. 1982. Purification and characterization of cellulolytic enzymes from Aspergillus niger. J. Korean Agric. Chem. Soc. 24: 186-192.
23 Park SK, Moon IS, Choi OJ, Sung NK. 1993. Isolation of ${\beta}$-glucosidase-producing fungi and properties of its crude enzyme. Korean J. Appl. Microbiol. Biotechnol. 21: 440-445.
24 Yang SO, Chang PS, Baek BK, Hong SD, Lee JH. 2007. Changes of isoflavone distribution in soybeans using almond powder. Korean J. Food Sci. Technol. 39: 231-236.   과학기술학회마을
25 Sung CK, Lee SW, Park SK, Park JR, Moon IS. 1997. Purification and characterization of ${\beta}$-glucosidase from Aspergillus niger SFN-416. Korean J. Appl. Microbiol. Biotechnol. 25: 44-50.