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http://dx.doi.org/10.4191/KCERS.2008.45.1.605

High Throughput Magnetic Separation for Human DNA by Aminosilanized Iron Oxide Nanoparticles  

Kang, Ki-Ho (Eco.Biomaterials Laboratory, Korea Institute of Ceramic Engineering and Technology)
Chang, Jeong-Ho (Eco.Biomaterials Laboratory, Korea Institute of Ceramic Engineering and Technology)
Publication Information
Journal of the Korean Ceramic Society / v.45, no.10, 2008 , pp. 605-609 More about this Journal
Abstract
This work describes the preparation of functionalized magnetic nanoparticles(MNPs) and their bioapplication to human DNA separation. Silica coated MNPs were prepared by changing the volume ratio of tetraethyl orthosilicate(TEOS) for controlled coating thickness on the original nanoparticle of MNPs. The sol-gel process in silica coating on MNPs surface was adapted for relatively mild reaction condition, low-cost, and surfactant-free. And then amino functionalized magnetic nanoparticles were synthesized using amine groups as surface modifiers. The result of adsorption efficiency for human DNA with amino-functionalized silica coated MNPs was calculated as a function of the number of amine groups.
Keywords
DNA; Magnetic separation; Nanoparticle; TEOS; Sol-gel;
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