Browse > Article
http://dx.doi.org/10.11002/kjfp.2017.24.8.1149

Anti-inflammatory effects of Ishige sinicola ethanol extract in LPS-induced RAW 264.7 cell and mouse model  

Kim, Ji-Hye (Department of Food Science and Technology/Institute of Food Science, Pukyong National University)
Kim, Min-Ji (Department of Food Science and Technology/Institute of Food Science, Pukyong National University)
Kim, Koth-Bong-Woo-Ri (Institute of Fisheries Sciences, Pukyong National University)
Park, Sun-Hee (Department of Food Science and Technology/Institute of Food Science, Pukyong National University)
Cho, Kwang-Su (Department of Food Science and Technology/Institute of Food Science, Pukyong National University)
Kim, Go-Eun (Department of Food Science and Technology/Institute of Food Science, Pukyong National University)
XU, Xiaotong (Department of Food Science and Technology/Institute of Food Science, Pukyong National University)
Lee, Da-Hye (Department of Food Science and Technology/Institute of Food Science, Pukyong National University)
Park, Ga-Ryeong (Department of Food Science and Technology/Institute of Food Science, Pukyong National University)
Ahn, Dong-Hyun (Department of Food Science and Technology/Institute of Food Science, Pukyong National University)
Publication Information
Food Science and Preservation / v.24, no.8, 2017 , pp. 1149-1157 More about this Journal
Abstract
Inflammation is the first response of the immune system to infection or irritation in our body. The use of medicinal plants has been widely applied as an alternative source for drug development. One of marine natural resources, the anti-inflammatory effect of Ishige sinicola ethanol extract (ISEE), was evaluated by using LPS-induced RAW 264.7 cell and mice model. As a result, the production of nitric oxide (NO) and pro-inflammatory cytokines (IL-6, IL-$1{\beta}$, TNF-${\alpha}$) were inhibited with increasing concentration of ISEE without any cytotoxicity. Furthermore, ISEE suppressed the expression of not only inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), nuclear factor-kappa B (NF-${\kappa}B$) p65, and mitogen-activated protein kinases (MAPKs), including extracellular signal-regulated kinase (ERK) 1/2, p38, and c-Jun N-terminal kinase (JNK) in a dose-dependent manner. In mice ear edema test, the formation of edema was reduced at the highest dosage of ISEE and the reduction of the number of infiltrated mast cells was observed in histological analysis. These results indicate that ISEE has a potent anti-inflammatory activity and can be used as a pharmaceutical material for many kinds of inflammatory disease.
Keywords
Ishige sinicola ethanol extract; proinflammatory cytokines; nuclear factor kappa B; mitogen-activated protein kinases;
Citations & Related Records
Times Cited By KSCI : 11  (Citation Analysis)
연도 인용수 순위
1 Nathan CF (1987) Secretory products of macrophages. J Clin Invest, 79, 319-326   DOI
2 Jaffrey SR, Snyder SH (1995) Nitric oxide: a neural messenger. Annu Rev Cell Dev Biol, 11, 417-440   DOI
3 Ghosh S, Hayden MS (2008) New regulators of NF-${\kappa}B$ in inflammation. Nat Rev Immunol, 8, 837-848   DOI
4 Storck M, Schilling M, Burkhardt K, Prestel R, Abendroth D, Hammer C (1994) Production of proinflammatory cytokines and adhesion molecules in ex-vivo xenogeneic kidney perfusion. Transpl Int, 7, S647-S649   DOI
5 Majdalawieh A, Ro HS (2010) Regulation of $I{\kappa}B{\alpha}$ function and NF-${\kappa}B$ signaling: AEBP1 is a novel proinflammatory mediator in macrophages. Mediators Inflammation, 2010, 1-27
6 Yang H, Oh KH, Yoo YC (2015) Anti-inflammatory effect of hot water extract of aronia fruits in LPS-stimulated RAW 264.7 macrophages. J Korean Soc Food Sci Nutr, 44, 7-13   DOI
7 Nagayama K, Iwamura Y, Shibata T, Hirayama I, Nakamura T (2002) Bactericidal activity of phlorotannins from the brown alga Ecklonia kurome. J Antimicrob Chemother, 50, 889-893   DOI
8 Ryu BH, Kim DS, Cho KJ, Sin DB (1989) Antitumor activity of seaweeds toward sarcoma-180. Korean J Food Sci Technol, 21, 595-600
9 Choo KS, Lee HN, Shin SA Kim HJ, Park YS, Kim SK, Jung JY (2016) Induction of growth inhibition and apoptosis in human cancer cells by a brown algae extract. J Life Sci, 26, 555-562   DOI
10 Cho BO, Ryu HW, So YK, Jin CH, Byun MW, Kim WG, Jeong IY (2012) Ishige sinicola extracts induce apoptosis via activation of a caspase cascade in Human hela cells. J Korean Soc Food Sci Nutr, 41, 901-906   DOI
11 Kim MJ, Bae NY, Kim KBWR, Kim JH, Park SH, Jang MR, Ahn DH (2016) Anti-inflammatory effect of Chondrus nipponicus yendo ethanol extract on lipopolysaccharide-induced inflammatory responses in RAW 264.7 cells. J Korean Soc Food Sci Nutr, 45, 194-201   DOI
12 Kim MJ, Bea NY, Kim KBWR. Park JH, Park SH, Cho YJ, Ahn DH (2015) Anti-inflammatory effect of water extract from tuna heart on lipopolysaccharide-induced inflammatory responses in RAW 264.7 Cells. Korean Soc Biotechnol Bioeng J, 30, 326-331
13 Kim MJ, Bae NY, Kim KBWR, Park JH, Park SH, Choi JS, Ahn DH (2016) Anti-inflammatory effect of Grateloupia imbricata Holmes ethanol extract on LPS-induced RAW 264.7 cells. J Korean Soc Food Sci Nutr, 45, 181-187   DOI
14 Clark D, Durner J, Navarre DA, Klessig DF (2000) Nitric oxide inhibition of tobacco catalase and ascorbate peroxidase. MPMI, 13, 1380-1384   DOI
15 Weisz A, Cicatiello L, Esumi H (1996) Regulation of the mouse inducible-type nitric oxide synthase gene promoter by interferon-${\gamma}$, bacterial lipopolysaccharide, and NG-monomethyl-L-arginine. Biochem J, 316, 209-215   DOI
16 Szelenyi J (2001) Cytokines and the central nervous system. Brain Res Bull, 54, 329-338   DOI
17 Choi YS, Won JY, Yoo IW, Shin JW, Kim SJ, Sohn NW (2013) Effect of tetramethylpyrazine on proinflammatory cytokine expressions in mouse brain tissue following intracerebroventricular lipopolysaccharide treatment. Korea J Herbol, 28, 83-90
18 Hinz B, Brune K (2002) Cyclooxygenase-2-10 years later. J Pharmacol Exp Ther, 300, 367-375   DOI
19 Li Y, Wu Q, Deng Y, Lv H, Qiu J, Chi G, Feng H (2015) D(-)-Salicin inhibits the LPS-induced inflammation in RAW 264.7 cells and mouse models. Int Immunopharmacol, 26, 286-294   DOI
20 Park, SH, Jeong JH, Park BJ, Jeong TH (2015) Anti-inflammatory effect of Fallopia sachalinensis RonseDecr. fruit. J Soc Cosmet Sci Korea, 41, 237-241
21 Kim SH, Yun JW, Lee YH, Cheon EJ (2009) Polymorphisms of tumor necrosis factor-alpha promoter gene in Kawasaki disease and relation to the risk of coronary artery lesion. Korean J Pediatr, 52, 476-480   DOI
22 Kim DH, Hwang EY, Son JH (2013) Anti-inflammatory activity of Carthamus tinctorious seed extracts in RAW264.7 cells. J Life Sci, 23, 55-62   DOI
23 Noh KH, Jang JH, Min KH, Chinzoring R, Lee MO, Song YS (2011) Suppressive effect of green tea seed coat ethyl acetate fraction on inflammation and its mechanism in RAW 264.7 macrophage cell. J Korean Soc Food Sci Nutr, 40, 625-634   DOI
24 Bak MJ, Jeong JH, Kang HS, Jin KS, Ok S, Jeong WS (2009) Cedrela sinensis leaves suppress oxidative stress and expressions of iNOS and COX-2 via MAPK signaling pathways in RAW 264.7 cells. J Food Sci Nutr, 14, 269-276
25 Kim BW, Kim JI, Kim HR, Byun DS (2014) Anti-inflammatory effect of an ethyl acetate fraction from Myagropsis yendoi on lipopolysaccharides- stimulated RAW 264.7 Cells. Korean J Fish Aquat Sci, 47, 527-536
26 Chung HJ, Lee HS, Shin JS, Lee SH, Park BM, Youn YS, Lee SK (2010) Modulation of acute and chronic inflammatory processes by a traditional medicine preparation GCSB-5 both in vitro and in vivo animal models. J Ethnopharmacol, 130, 450-459   DOI
27 Ljung T, Lundberg S, Varsanyi M, Ohansson C, Schmidt PT, Herulf M, Lundberg JO, Hellstrom PM (2006) Rectal nitric oxide as biomarker in the treatment of inflammatory bowel disease: responders versus non-responder. World J Gastroenterol, 12, 3386-3392   DOI
28 Eum WS, Lee KJ, Kim DW, Lim SS, Kang IJ, Park J, Choi SY (2013) Anti-inflammatory effects of extracts from Caesalpinia sappan L. on skin inflammation. J Korean Soc Food Sci Nutr, 42, 384-388   DOI
29 Amin K (2012) The role of mast cells in allergic inflammation. Respir Med, 106, 9-14   DOI
30 Ferrero-Miliani L, Nielsen OH, Andersen PS, Girardin SE (2007) Chronic inflammation: importance of NOD2 and NALP3 in interleukin-$1{\beta}$ generation. Clin Exp Immunol, 147, 227-253
31 Wu MJ, Wang L, Ding HY, Weng CY, Yen JH (2004) Glossogyne tenuifolia acts to inhibit inflammatory mediator production in a macrophage cell line by downregulating LPS-induced NF-${\kappa}B$. J Biomed Sci, 11, 186-199
32 Rocca B, FitzGerald GA (2002) Cyclooxygenases and prostaglandins: shaping up the immune response. Int Immunopharmacol, 2, 603-630   DOI
33 Seo UK, Lee JI, Park JH, Park YK (2008) The ethyl acetate extract of north Kangwhal (Ostericum koreanum) attenuates the inflammatory responses in PMA/A23187-stimulated mast cells. Korea J Herbol, 23, 81-89