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http://dx.doi.org/10.11002/kjfp.2017.24.6.842

Antioxidant and anti-inflammatory effects of phenolic rich Hylotelephium erythrostictum extracts  

Kim, Sang Suk (Citrus Research Institute, National Institute of Horticultural and Herbal Science, RDA)
Park, Kyung Jin (Citrus Research Institute, National Institute of Horticultural and Herbal Science, RDA)
Lee, Seung Eun (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA)
Lee, Jeong Hoon (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA)
Choi, Young Hun (Citrus Research Institute, National Institute of Horticultural and Herbal Science, RDA)
Publication Information
Food Science and Preservation / v.24, no.6, 2017 , pp. 842-848 More about this Journal
Abstract
Hylotelephium erythrostictum is commonly used as a medicinal herb. In this study, H. erythrostictum leaf (HEL), branch (HEB), root (HER), and above ground (HEAG) extracts were evaluated for their antioxidant properties. The antioxidant activities were assayed by three methods based on scavenging of DPPH, ABTS and superoxide anion radical. HEAG extract showed the highest DPPH, ABTS, superoxide anion radical scavenging activities. HEAG extract also exhibited the highest phenolic content (230 mg/g gallic acid equivalent). In our research for anti-inflammatory ingredients, the extract of HEAG inhibited the generation of nitric oxide (NO) in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophage cells. To test the inhibitory effects of HEAG on pro-inflammatory cytokines, we conducted ELISA assay for the measuring the generation of tumor necrosis factor $(TNF)-{\alpha}$, IL (interleukin)-$1{\beta}$, and IL(interleukin)-6 in LPS-stimulated RAW264.7 macrophage cells. In these assays, HEAG ethanol extract showed a dose-dependent decrease in the production of $TNF-{\alpha}$, $IL-1{\beta}$, and IL-6. Based on these results, extract of HEAG could be the efficient candidate for anti-inflammatory agents.
Keywords
Hylotelephium erythrostictum; anti-inflammatory; nitric oxide; cytokine;
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