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http://dx.doi.org/10.11002/kjfp.2016.23.7.1026

Citrus platymamma inhibits the expression of pro-inflammatory cytokines, inducible nitric oxide synthase, and cyclooxygenase-2 in RAW264.7 macrophage  

Kim, Sang Suk (Citrus Research Institute, National Institute of Horticultural and Herbal Science, RDA)
Park, Kyung Jin (Citrus Research Institute, National Institute of Horticultural and Herbal Science, RDA)
An, Hyun Joo (Citrus Research Institute, National Institute of Horticultural and Herbal Science, RDA)
Choi, Young Hun (Citrus Research Institute, National Institute of Horticultural and Herbal Science, RDA)
Publication Information
Food Science and Preservation / v.23, no.7, 2016 , pp. 1026-1032 More about this Journal
Abstract
Citrus platymamma hort. ex Tanaka is widely used in traditional Korean medicine because of its medicinal benefits including an anti-inflammatory effect. This study aimed to evaluate changes in the flavonoid content and anti-inflammatory activities of C. platymamma during its harvest period. Fruit peel samples were obtained between September 2015 and February 2016. The results indicate that C. platymamma peel extract (CPE) was an effective inhibitor of lipopolysaccharide (LPS)-induced NO production in RAW264.7 cells. The inhibitory effects of CPE at $100{\mu}g/mL$ concentration included dose-dependent decreases in the expression of iNOS and COX-2 proteins. In addition, CPE decreased the expression of pro-inflammatory cytokines TNF-${\alpha}$, IL-$1{\beta}$, and IL-6. The highest anti-inflammatory activity and flavonoid content were observed in CPE of C. platymamma peel harvested during the immature fruit period in September. Further, to assess the suitability of CPE for cosmetic use, we performed MTT assays using HaCaT keratinocytes and observed that CPE did not exhibit any cytotoxicity. To test the potential application of CPE as a cosmetic material, we also performed primary skin irritation tests on normal skin of 30 volunteers and no adverse reactions were observed. The results of this study indicate that CPE may be considered as an anti-inflammatory candidate for inclusion in cosmetic materials.
Keywords
Citrus platymamma; anti-inflammatory; cytokine; iNOS; COX-2;
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1 Ham YM, Yoon WJ, Lee WJ, Kim SC, Baik JS, Kim JH, Lee GS, Lee NH, Hyun CG (2015) Anti-inflammatory effects of isoketocharbroic acid from brown alga, Sargassum micracanthum. EXCLI J, 14, 1116-1121
2 Koh JS (2001) Citrus Industry. Jeju Munhwasa, Jeju, Korea, p 163-172
3 Singleton VL, Orthofer R, Lamuela-Raventos RM (1999) Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods Enzymol, 299, 152-178   DOI
4 Blois MS (1958) Antioxidant determination by use of a stable free radical. Nature, 181, 1199-1200   DOI
5 Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C (1999) Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med, 26, 1231-1237   DOI
6 Benzie IFF, Strain JJ (1996) The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power": The FRAP assay. Anal Biochem, 239, 70-76   DOI
7 Harborne JB, Williams CA (2000) Advances in flavonoid research since 1992. Phytochemistry, 55, 481-504   DOI
8 Hertog MGL, Feskens EJM, Hollman PC, Katan MB, Kromhout D (1993) Dietary antioxidant flavonoids and risk of coronary heart disease: the Zutphen Eiderly Study. The Lancet, 342, 1007-1011   DOI
9 Chae WP, Xing ZC, Kim YJ, Sang HS, Huh MW, Kang IK (2011) Fabrication and biocompatibility of rutincontaining PHBV nanofibrous scaffolds. Polymer (Korea), 35, 210-215   DOI
10 Guo S, Qiu P, Xu G, Wu X, Dong P, Yang G, Zheng J, McClements DJ, Xiao H (2012) Synergistic anti-inflammatory effects of nobiletin and sulforaphane in lipopolysaccharide-stimulated RAW 264.7 cells. J Agric Food Chem, 60, 2157-2164   DOI
11 Horowitz RM, Gentili B (1977) Flavonoid constituents of citrus. In: Citrus Science and Technology. Nagy S, Shaw SPE, Veldhuis MK (Editor), AVI Publishing Co., Inc., Westport, CT, USA, p 397-426
12 Gattuso G, Barreca D, Gargiulli C, Leuzzi U, Caristi C (2007) Flavonoid composition of citrus juices. Molecules, 12, 1641-1673   DOI
13 Nogata Y, Sakamoto K, Shiratsuchi H, Ishii T, Yano M, Ohta H (2006) Flavonoid composition of fruit tissues of citrus species. Biosci Biotechnol Biochem, 70, 178-192   DOI
14 Lee SG, Ra JH, Song JY, Gwak CH, Kwon HJ, Yim SV, Hong SP, Kim JJ, Lee KH, Cho JJ, Park YS, Park CS, Ahn HJ (2011) Extracts from Citrus unshiu promote immune-mediated inhibition of tumor growth in a murine renal cell carcinoma model. J Ethnopharmacol, 133, 973-979   DOI
15 Li S, Pan MH, Lo CY, Tan D, Wang Y, Shahidi F, Ho CT (2009) Chemistry and health effects of polymethoxyflavones and hydroxylated polymethoxyflavones. J Funct Foods, 1, 2-12   DOI
16 Shih MF, Cheng YD, Shen CR, Cherng JY (2010) A molecular pharmacology study into the anti-inflammatory actions of Euphorbia hirta L. on the LPS-induced RAW 264.7 cells through selective iNOS protein inhibition. J Nat Med, 64, 330-335   DOI
17 Tsatsanis C, Androulidaki A, Venihaki M, Margioris AN (2006) Signalling networks regulating cyclooxygenase-2. Int J Biochem Cell Biol, 38, 1654-1661   DOI
18 Kim MJ, Kim SS, Park KJ, An HJ, Choi YH, Lee NH, Hyun CG (2016) Methyl jasmonate inhibits lipopolysaccharide-induced inflammatory cytokine production via mitogen-activated protein kinase and nuclear factor-${\kappa}B$ pathways in RAW264.7 cells. Pharmazie, 71, 540-543
19 Morwood CJ, Lappas M (2014) The citrus flavone nobiletin reduces pro-inflammatory and pro-labour mediators in fetal membranes and myometrium: implications for preterm birth. PLoS One, 9, e108390   DOI
20 Yoshimizu N, Otani Y, Saikawa Y, Kubota T, Yoshida M, Furukawa T, Kumai K, Kameyama K, Fujii M, Yano M, Sato T, Ito A, Kitajima M (2004) Anti-tumour effects of nobiletin, a citrus flavonoid, on gastric cancer include: antiproliferative effects, induction of apoptosis and cell cycle deregulation. Aliment Pharmacol Ther, 20, 95-101
21 Walle T (2007) Methoxylated flavones, a superior cancer chemopreventive flavonoid subclass?. Semin Cancer Biol, 17, 354-362   DOI
22 Suzuki M, Sasaki K, Yoshizaki F, Fujisawa M, Oguchi K, Cyong JC (2005) Anti-hepatitis C virus effect of Citrus unshiu peel and its active ingredient nobiletin. Am J Chin Med, 33, 87-94   DOI
23 Bracke ME, Vyncke BM, Van Larebeke NA, Bruyneel EA, De Bruyne GK, De Pestel GH, De Coster WJ, Espeel MF, Mareel MM (1989) The flavonoid tangeretin inhibits invasion of MO4 mouse cells into embryonic chick heart in vitro. Clin Exp Metastasis, 7, 283-300   DOI
24 Park YC, Yang YT, Kim JY, Lee CH, Kang SH, Kang JH (2015) Characteristics of flavonoids in juice and cluster analysis of satsuma mandarin germplasms. Korean J Plant Res, 28, 16-25   DOI